Structure of PDB 8jke Chain F Binding Site BS01

Receptor Information

>8jke Chain F (length=301) Species: 100226 (Streptomyces coelicolor A3(2))

ATADPVKDYLKQIGKVPLLNAEQEVELAKRIEAGLFAEDKLANSDKLAPK
LKRELEIIAEDGRRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQEGNL
GLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVI
NKLARVQRQMLQDLGREPTPEELAKELDMTPEKVIEVQKYGREPISLHTP
LGEDGDSEFGDLIEDSEAVVPADAVSFTLLQEQLHSVLDTLSEREAGVVS
MRFGLTDGQPKTLDEIGKVYGVTRERIRQIESKTMSKLRHPSRSQVLRDY
L

Ligand information

>8jke Chain O (length=61)

ccggagcgttcagcgttcgtttatctccccctggcactgtcatctccgtc
agaccgtcgca

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8jke Structural and functional characterization of AfsR, a SARP family transcriptional activator of antibiotic biosynthesis in Streptomyces
• Resolution: 3.67 Å
• Binding residue (original residue number in PDB): V215 K216 R284 L285 K317 F318 D319 K322 Y324 K325 S327 T328 Y329 W332 W333 Q336 R340 R350 H354
• Binding residue (residue number reindexed from 1): V6 K7 R75 L76 K108 F109 D110 K113 Y115 K116 S118 T119 Y120 W123 W124 Q127 R131 R141 H145

Gene Ontology

Molecular Function

• GO:0001108 bacterial-type RNA polymerase holo enzyme binding
• GO:0003677 DNA binding
• GO:0003700 DNA-binding transcription factor activity
• GO:0005515 protein binding
• GO:0016987 sigma factor activity

Biological Process

• GO:0006352 DNA-templated transcription initiation
• GO:0006355 regulation of DNA-templated transcription
• GO:0010468 regulation of gene expression
• GO:2000142 regulation of DNA-templated transcription initiation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8jke, PDBe:8jke, PDBj:8jke
• PDBsum: 8jke
• PubMed: 38427710
• UniProt: P18183|SIGA_STRCO RNA polymerase principal sigma factor HrdB (Gene Name=hrdB)