Structure of PDB 8c94 Chain F Binding Site BS01

Receptor Information
>8c94 Chain F (length=177) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR
Ligand information
>8c94 Chain A (length=1668) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucaccgggagauagc
ugguucuccccgaaagcuauuuagguagcgccucgugaauucaucuccgg
ggguagagcacuguuucggcccgaugcaaacugcgaauaccggagaaugu
uaucacgggagacacacggcgggugcuaacguccgucgugaagagggaaa
caacccagaccgccagcuaaggucccaaagucaugguuaagugggaaacg
augugggagccugcgcggauaacggggcuaaaccaugcaccgaagcugcg
gcagcgacgcuuaugcguuguuggguaggggagcguucuguaagccugcg
aaggugugcugugaggcaugcuggagguaucagaagugcgaaugcugaca
uaaguaacaaagcgggugaaaagcccgcucgccggaagaccaagugguca
gguagagaauaccaaggcgcugagagaacucgggugaaggaacagacuca
gugaaauugaacucgcugugaagaugcaguguacccgcggcaagacggaa
aguccuccuaaagaguaacggaggagcacgaagguuggcuaauccugguc
ggacaucaggagguuagugcaauggcauaagccagcuugacugcgagcgu
gacggcgcgagcaggugcgaaagcaggucauagugauccggugguucuga
auggaagggccaucgcucaacggucccuaucugccgugggcgcuggagaa
cugaggggggcugcuccuaguacgagaggaccggaguggacgcaucacug
guguucggguugucaugccaauggcacugcccgguagcuaaaugcggaag
agauaagugcugaaagcaucuaagcacgaaacuugccccgagaugaguuc
ucccugacccuuuaaggguccugaaggaacguugaagacgacgacguuga
uaggccggguguguaagcgcagcgaugcguugagcuaaccgguacuaaug
aaccgugaggcuuaaccu
<<<<<<<<......<<<<<<<<<<<....<<<..<<<<<<<<<<<<<...
...<<<<<<....(((.>>>.....<<<<<<<..<<<)))....>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<......
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..<<<..<<<<......<<<<<<<<<<<<<.<<<...
<<<<<......>>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<..<<<<.........>>>>..>>.
.....<<<<<..{.[[>>>>>..>>>......>>.>>>>>>>.>>.....
..........<<....>>....>>.........<<<<.....>>>>....
.........>....>>>>>>>>>>>.......<<<<..<<<<<......>
>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<..<<<<<<<....>>>>>>...>..>
.>>..<<<<<<....<<<<<....>>>>>..<<<<<<<...<<<<<<<.<
<....<<<...<<<.<<<<.>>>>.>>>..>>.>.....>>>>>>.>>>.
..>>>>>>>.....<<<<<<<<<<......>>>>>>>>>>....<<<...
...>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<
..<<<<<..>>>>>.>>......))).....>>>>>>.>>>...>>>>>>
><<<<<<<<<....>>>>>>>>>....>>>>>><<<<<<<...<<<....
..<<<<<<<<....>>>>>>>>...>>>..>>>>>.>>.....>>>>>><
......<<..<<<<<<.......>>>>>><<<<....<<<<<..>>>>>.
..<<.....>>....>>>>.<<<<....<<.............>>.>>>>
>>.....>.....<<<<<........>>>>>..<<<<<<<<.<<<.<<..
..<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<..
.....>>>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<<
....>>>>..>>>>.<<<....>>>.>>))).......<<<<<<<.<<..
]]}...>>>>>>>>>..........>>..>>>>>>>>>>>....<<<<<<
<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<
<<...<<<<<<<<<<<....>>>>>>..>>>>>...>>>>.>>>>>...<
<<....<<<<<...........>>>>>....>>>.>>>>.......>>>>
>>>..<<<<<.....>>>>><....<<..<<<<<.......>>>>>....
<<<<<<<<..<<....<<<<<....>>>>>...>>...>>>>>.>>>...
..>>..>..>>>>>>>>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8c94 Cryo-EM captures early ribosome assembly in action.
Resolution3.8 Å
Binding residue
(original residue number in PDB)		T34 N36 G38 V39 G40 E41 T67 R70 K71 F76 I78 I84 K87 K119 D122 R124 M129 R132 G150 T154
Binding residue
(residue number reindexed from 1)		T34 N36 G38 V39 G40 E41 T67 R70 K71 F76 I78 I84 K87 K119 D122 R124 M129 R132 G150 T154
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0008097 5S rRNA binding
GO:0019843 rRNA binding
Biological Process
GO:0000027 ribosomal large subunit assembly
GO:0002181 cytoplasmic translation
GO:0006412 translation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:8c94, PDBe:8c94, PDBj:8c94
PDBsum8c94
PubMed36797249
UniProtP62399|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)

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