Structure of PDB 7ohx Chain F Binding Site BS01

Receptor Information

>7ohx Chain F (length=241) Species: 559292 (Saccharomyces cerevisiae S288C)

EKILTPESQLKKSKAQQKTAEQVAAERAARKAANKEKRAIILERNAAYQK
EYETAERNIIQAKRDAKAAGSYYVEAQHKLVFVVRIKGINKIPPKPRKVL
QLLRLTRINSGTFVKVTKATLELLKLIEPYVAYGYPSYSTIRQLVYKRGF
GKINKQRVPLSDNAIIEANLGKYGILSIDDLIHEIITVGPHFKQANNFLW
PFKLSNPSGGWGVPRKFKHFIQGGSFGNREEFINKLVKSMN

Ligand information

>7ohx Chain 1 (length=1561)

guuugaccucaaaucagguaggaguacccgcugaauaucaauaagcggag
gaaaagaaaccaaccgggauugccuuaguaacgcgagugaagcggcaaaa
gcucaaauuugaaaucugguaccuucggugcccgaguuguaauuuggaga
gggcacuuuggggccguuccuugucuauguuccuuggaacaggacgucau
agagggugagaaucccguguggcgaggagugcgguucuuuguaaagugcc
uucgaagagucgaguuguuuggaugcagcucuaagugggugguuccaucu
aaagcuaaauauuggcgagagaccgauagcgaacaguacagugauggaaa
gaugaaaagaacuuugaaaagagagugaaaaaguacgugaaauuguugaa
aggaagggcauuugaucagacaugguguuuugugcccucugcuccuugug
gguaggggaucucgcauuucacugggccagcaucaguuuugguggcagga
uaaauccauaggaauguagcuugccgguaaguauuauagccugugggaau
acugccagcugggacugaggacugcgacguaagucaaggaugcuggcaua
augguuauaugccgcccgucuugaaacacggaccaaggagucuaacgucu
augcgaguguuuggguguaaaacccauacgcguaauugaacguagguugg
ggccucgcaagaggugcacaaucgaccgauccuggauggauuugguaaga
gcauagcuguugggacccgaaagauggugaaaaccaucuaguacugguuc
cugccgaaguuucccucaggauagcagaagcucguaucaguuuuauguaa
gaaugaagagcuuuuagugggccauuuuugguaagcagaacuggcgaugc
gggaugaaccgaacguagaguuaaggugccggaauacacgcucaagaaca
aaauaguucaucuagaccggguggccauggaagucggaauccgcuaagga
guguguaacaaccggaugaacuagcccugggcgcucaagcguguuaccua
uacucuaccgucaggguugauaugaugcccugacgaguaggcaggcgugg
aggucagugacgaagccuagaccguaaggucgggucgaacggccucuagu
gcagaucuuggugguaguagcagcgaaaccacaccaaggggcuuggcuac
cgaagcagaaguaauguuaccgcaauaguaauugaacuuaguacgagagg
aacaguucauucggauaauugguuuuugcggcugucucaggcagccgcga
agcuaccauccgcuggauuauggcugaacccucuaagucagauccaugcu
agaagcggugaugcuccacacagauggauacgaauaaggcguccuugugg
cgucgcugaaccauagcaggcuagcaacggugcacuuggcggaaaggccu
ugggugcuugcuggcgaauugcaaugcguggggauaaaucauuuguauac
gacuuagauguacaacgggguauuguaagcgcugagauuaagccuuuguu
gucugauuugu

..........................<<<<<<...........>>>>>.>
.........<<....>>..<<<<<......<<....>>.....>>>>>..
<<<...........<<.<<<<<<...>>>>>>.>>.............<<
<<<<<<<<<<<<<<<<<<<<<<<<<<....<<<<......>>>>.((...
...<<<.....))>>>..>.>>>>>>>>>..>>>>>>>....>>>>>>>>
>>>........<<<<<<<......>>>>>>>.....<<<<<<..>>>>>>
..>>>.................................<<....>>....
...........<<<......>>>...........................
......<<<<<.<<<<<<.....<<<<...<<<..<<<<<<<<<.....>
>>>>>>>>.....>>>...>>>>..<<<<<<<.<<<<<<<<<<<<<<<..
...<<<<<<<<<......<<<<<....>>>>>.......>>>>>>>.>.>
.>>>>>.>>>>>>>>>>........<<<....>>>.....>>>>>>>...
.>>>>>>.>>>>>..<<<<........>>>><<<<<<....<<<<<<.<<
<<<<..<<<...<<<<.....>>>>...>>>.............<<<<<.
.<<<<<....>>>>>...>>>>>.<<<...<<.....>>....>>>....
>>>>>>.>>>>>>..<<.<...<<<<<<....>>>>>>.....>.>>..<
<<<<<......<<......>>..<<<<<<<<<....<<<.<<<<<..>>>
.>>>>>..>>>>>>>.>>..<<<<.<.<<<.....>>>>..>>>>....<
<<......>>>...<<<<<<<..<<<<(((.....<<<<<<....<....
...<<<<<<<<......<<.....<<.........>>.............
..<<......>>>>.>>>>>>>>......))).>....>>>>>>.>>>>.
.>>>>>>><<<<<<<<<.........>>>>>>>>>....>>>>>><.<<<
<<<<<.<<......<<<<.<<<<....>>>>>>>>...>>>>>>>>>>..
>.....>>>>>><<.....<<.>>....>>...<<<<<...>>>>>.<<<
..........>>>..<<<<<<<<..<<<<.<.<<<<<<............
...>>>>>>.><<<<<...<<<<....<<<<<...........>>>>>..
.>>>>..>>>>>.<<<<...<<<<<..........>>>>>..>>>>.>>>
>...>>>>>>>>.<<<<<<......<<..........<<<<<<.....>>
>>>>......>>.......<<<<<<...<<<<<.<..<<<......>>>.
.>.>>>>>.>>>>>>............>>>>>>.<<<<<<<<........
.....>.....<<<<<<<<<.<<<....<..>...>>>....>>>.>>>>
>>.>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ohx Analysis of subunit folding contribution of three yeast large ribosomal subunit proteins required for stabilisation and processing of intermediate nuclear rRNA precursors.
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): I6 K14 K15 R30 R33 K34 N37 R41 R60 R67 K70 K90 G91 N93 P97 K98 K101 L105 R107 R110 I111 N112 L126 L129 S142 R145 Q159 R160 D165 K196 N200 K206 L207 S208 N209 P210 S211 G213 W214 P217 F220 K241
• Binding residue (residue number reindexed from 1): I3 K11 K12 R27 R30 K31 N34 R38 R57 R64 K67 K87 G88 N90 P94 K95 K98 L102 R104 R107 I108 N109 L123 L126 S139 R142 Q156 R157 D162 K193 N197 K203 L204 S205 N206 P207 S208 G210 W211 P214 F217 K238

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000463 maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000470 maturation of LSU-rRNA
• GO:0002181 cytoplasmic translation
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ohx, PDBe:7ohx, PDBj:7ohx
• PDBsum: 7ohx
• PubMed: 34813592
• UniProt: P05737|RL7A_YEAST Large ribosomal subunit protein uL30A (Gene Name=RPL7A)