Structure of PDB 7as8 Chain F Binding Site BS01

Receptor Information

>7as8 Chain F (length=206) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

TKGILGRKIGMTQVFAENGDLIPVTVIEAAPNVVLQKKTAENDGYEAIQL
GFDDKREKLSNKPEKGHVAKAETAPKRFVKELRGVEMDAYEVGQEVKVEI
FSAGEIVDVTGVSKGKGFQGAIKRHGQSRGPMSHGSRYHRRPGSMGPVDP
NRVFKGKLLPGRMGGEQITVQNLEIVKVDAERNLLLIKGNVPGAKKSLIT
VKSAVK

Ligand information

>7as8 Chain A (length=2812)

guuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaug
aaggacgggacgaacaccgauaugcuucggggagcuguaagcaagcuuug
auccggagauuuccgaauggggaaacccaccacucguaauggagugguau
ccauaucugaauucauaggauaugagaaggcagacccggggaacugaaac
aucuaaguacccggaggaagagaaagcaaaugcgauucccugaguagcgg
cgagcgaaacgggaucagcccaaaccaagaggcuugccucuugggguugu
aggacacucuguacggaguuacaaaggaacgagguagaugaagaggucug
gaaaggcccgccauaggagguaacagcccuguagucaaaacuucguucuc
uccugaguggauccugaguacggcggaacacgugaaauuccgucggaauc
cgggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaa
ccaguaccgugagggaaaggugaaaagcaccccggaaggggagugaaaga
gauccugaaaccgugugccuacaaguagucagagcccguuaacgggugau
ggcgugccuuuuguagaaugaaccggcgaguuacgaucucgugcaagguu
aagcagaagaugcggagccgcagcgaaagcgagucugaauagggcgcaug
aguacguggucguagacccgaaaccaggugaucuacccauguccagggug
aaguucagguaacacugaauggaggcccgaacccacgcacguugaaaagu
gcggggaugagguguggguaggggugaaaugccaaucgaaccuggagaua
gcugguucucuccgaaauagcuuuagggcuagccucaagguaagagucuu
ggagguagagcacugauuggacuaggggcccggguuaccgaauucaguca
aacuccgaaugccaaugacuuauccuugggagucagacugcgagugauaa
gauccguagucgaaagggaaacagcccagaccgccagcuaaggucccaaa
guauacguuaaguggaaaaggauguggaguugcuuagacaaccaggaugu
uggcuuagaagcagccaccauuuaaagagugcguaauagcucacuggucg
agugacucugcgccgaaaauguaccggggcuaaacguaucaccgaagcug
cggacuguucgaacagugguaggagagcguucuaagggcugugaagccag
accggaaggacugguggagcgcuuagaagugagaaugccgguaugaguag
cgaaagaggggugagaauccccuccaccgaaugccuaagguuuccugagg
aaggcucguccgcucaggguuagucgggaccuaagccgaggccgaaaggc
guaggcgauggacaacagguugauauuccuguaccaccuccucaccauuu
gagcaauggggggacgcaggaggauaggguaagcgcgguauuggauaucc
gcguccaagcaguuaggcugggaaauaggcaaauccguuucccaaaggcu
gagcugugauggcgagcgcgaaguuccugauuccacacugccaagaaaag
ccucuagcgaggugagaggugcccguaccgcaaaccgacacagguaggcg
aggagagaauccuaaggugaucgagagaacucucguuaaggaacucggca
aaaugaccccguaacuucgggagaaggggugcucuguuagggugcaagcc
cgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaacacag
gucucugcgaagccguaaggcgaaguauaggggcugacgccugcccggug
cuggaagguuaagaggagcgcuuagcguaagcgaaggugcgaauugaagc
cccaguaaacggcggccgucgguccuaagguagcgaaauuccuugucggg
uaaguuccgacccgcacgaaaggcgcaacgaucugggcacugucucaacg
agagacucggugaaauuauaguaccugugaagaugcagguuacccgcgac
aggacggaaagaccccguggagcuuuacugcagccugauauugaauguug
guuugaccuucuaacccgccgcccuuaucgggcggggagacagugucagg
ugggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgccc
aaagguucccucagaaugguuggaaaucauucgcagaguguaaaggcaca
agggagcuugacugcgagaccuacaagucgagcagggacgaaagucgggc
uuagugauccggugguuccgcauggaagggccaucgcucaacggauaaaa
gcuaccccggggauaacaggcuuaucucccccaagaguccacaucgacgg
ggagguuuggcaccucgaugucggcucaucgcauccuggggcuguagucg
gucccaaggguugggcuguucgcccauuaaagcgguacgcgagcuggguu
cagaacgucgugagacaguucggucccuauccgucgcgggcgcaggaaau
uugagaggagcuguccuuaguacgagaggaccgggauggacgcaccgcug
guguaccaguuguucugccaagggcaucgcuggguagcuaugugcggacg
ggauaagugcugaaagcaucuaagcaugaagccccccucaagaugagauu
ucccauuccgcaaggaaguaagaucccugaaagaugaucagguugauagg
ucugagguggaaguguggcgacacauggagcugacagauacuaaucgauc
gaggacuuaacc

<<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<....
..<<<<<<..[..((.>>>.....<<<<<<<..<<<))..]..>>>....
.>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<
<<<<<<<<.........>>>>>>>.>>.>>....<<<<<<.....<<...
.>>......>>>>>.>.........<<....>>...<<<<......<<..
...>>.....>>>>...(((....<<<<<<<<....>>>>>>>>......
<<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<.....<<<<..
....>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>>>
>>>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>.....
.<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].........
......<<....>>....]].........<<<<....>>>>.........
....}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>
>>>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<.
..<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....
.>>>>>.>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<..
..<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>
>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>.
.>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<
<<.....<<<...<<<<<<<<...<<<<<<..>>>>.>>...>>>>>>>>
..>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<.<<.....
.>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((...
..<<<<<<...........<<..<<<<<<<<<........<<<<<.<<..
.<<<<.......>>>>...>>.........<<......>>...>>>>>..
..>>>>>>>>>.>>...........))).....>>>>>>.>>>...>>>>
>>><<<<<<..>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<
.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<
<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<.
..<<.....>>.>>>>>>....((<<<<.<<.<.<<<...<<<....>>>
...>>>..>>>...<<<<<........>>>>>))<<<<..<<<<<<....
..<<...<<<<....<<<<..<<<..<<<.<<<<<<<<...(((....>>
>>>><<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>...>>>>
..>>>>>...>><..<..>...>>>>>>...>>>...>>>>.........
>>>>..>>..>>>>>>.>>>>>>>>..>>.....<<......>>>>>>>.
<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.........<
.....<<<<<....<....>.....>>>>><<<<<....<<<<....>>>
>...>>>>>............<<<<<<<<<..<<<.........>>>..<
<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..<<<
<<<<..<<<<..<<...<<<<<<.<<....>>..>>>>>>...>>..>>>
>>>>>>..>>>>><<<<<.>>>>>........<...........<<<<<<
.....>>>>>>.....>....>>>>...>>.>>>>>>>..>..>>.>>>>
>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<<<
.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<.<<<<
...>>>>.>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>
>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<
...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>...
.>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>)
))]......<<<<<<<.((..>>>>..))>>>>>>>.............>
>>>..]]>>>>.......<<<<...<<<<<<...<<.......>>...>>
>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>
>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>..
..<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<
<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<
<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<
<<....<<<<<...........>>>>>....>>>.>>>>.......>>>>
>>>..<<<<....>>>>....<<<<<<<<<.......>>>>>....<<<<
<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>
....>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7as8 Structural Basis for Bacterial Ribosome-Associated Quality Control by RqcH and RqcP.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): M12 T13 Q14 P24 Q37 D44 Y46 Q50 R57 K59 L60 N62 K63 P64 H68 R78 V80 K81 E82 R84 K115 G116 K117 G118 F119 Q120 G121 K124 R125 S129 R130 P132 S134 H135 G136 R138 Y139 H140 R141 P143 G144 S145 M146 G147 P148 V149 D150 P151 N152 R153 V154 F155 K158 L160 P161 R163 Q168 T170 Q172 N173 K189 N191 V192 P193 K207
• Binding residue (residue number reindexed from 1): M11 T12 Q13 P23 Q36 D43 Y45 Q49 R56 K58 L59 N61 K62 P63 H67 R77 V79 K80 E81 R83 K114 G115 K116 G117 F118 Q119 G120 K123 R124 S128 R129 P131 S133 H134 G135 R137 Y138 H139 R140 P142 G143 S144 M145 G146 P147 V148 D149 P150 N151 R152 V153 F154 K157 L159 P160 R162 Q167 T169 Q171 N172 K188 N190 V191 P192 K206

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:2000234 positive regulation of rRNA processing

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7as8, PDBe:7as8, PDBj:7as8
• PDBsum: 7as8
• PubMed: 33259810
• UniProt: P42920|RL3_BACSU Large ribosomal subunit protein uL3 (Gene Name=rplC)