Structure of PDB 6m7j Chain F Binding Site BS01

Receptor Information

>6m7j Chain F (length=319) Species: 1773 (Mycobacterium tuberculosis)

SEALRQARKDAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLY
ATQLMTELSERGEKLPAAQRRDMMWICRDGDRAKNHLLEANLRLVVSLAK
RYTGRGMAFLDLIQEGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRA
MADQARTIRIPVHMVEVINKLGRIQRELLQDLGREPTPEELAKEMDITPE
KVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVDAVSFTLLQD
QLQSVLDTLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIES
KTMSKLRHPSRSQVLRDYL

Ligand information

>6m7j Chain O (length=31)

gcttgacaaaagtgttaaattgtgctatact

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6m7j Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
• Resolution: 4.4 Å
• Binding residue (original residue number in PDB): L240 A298 N299 R301 L302 S305 R330 K334 F335 D336 K339 Y341 K342 S344 T345 Y346 W349 W350 Q353 R357 R367 P369 H371 T499 E501 R502 Q505
• Binding residue (residue number reindexed from 1): L32 A90 N91 R93 L94 S97 R122 K126 F127 D128 K131 Y133 K134 S136 T137 Y138 W141 W142 Q145 R149 R159 P161 H163 T291 E293 R294 Q297

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003700 DNA-binding transcription factor activity
• GO:0005515 protein binding
• GO:0016987 sigma factor activity

Biological Process

• GO:0006352 DNA-templated transcription initiation
• GO:0006355 regulation of DNA-templated transcription
• GO:0009415 response to water
• GO:0010468 regulation of gene expression
• GO:2000142 regulation of DNA-templated transcription initiation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0009274 peptidoglycan-based cell wall

External links

• PDB: RCSB:6m7j, PDBe:6m7j, PDBj:6m7j
• PDBsum: 6m7j
• PubMed: 30626968
• UniProt: P9WGI1|SIGA_MYCTU RNA polymerase sigma factor SigA (Gene Name=sigA)