Structure of PDB 4x4t Chain F Binding Site BS01

Receptor Information

>4x4t Chain F (length=437) Species: 224325 (Archaeoglobus fulgidus DSM 4304)

MKVEEILEKALELVIPDEEEVRKGREAEEELRRRLDELGVEYVFVGSYAR
NTWLKGSLEIDVFLLFPEEFSKEELRERGLEIGKAVLDSYEIRYAEHPYV
HGVVKGVEVDVVPCYKLKEPKNIKSAVDRTPFHHKWLEGRIKGKENEVRL
LKGFLKANGIYGAEYKVRGFSGYLCELLIVFYGSFLETVKNARRWTRRTV
IDVAKGEVRKGEEFFVVDPVDEKRNVAANLSLDNLARFVHLCREFMEAPS
LGFFKPKHPLEIEPERLRKIVEERGTAVFAVKFRKPDIVDDNLYPQLERA
SRKIFEFLERENFMPLRSAFKASEEFCYLLFECQIKEISRVFRRMGPQFE
DERNVKKFLSRNRAFRPFIENGRWWAFEMRKFTTPEEGVRSYASTHWHTL
GKNVGESIREYFEIISGEKLFKEPVTAELCEMMGVKD

Ligand information

>4x4t Chain G (length=18)

ggccgcggcgccgcgauc

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Receptor-Ligand Complex Structure

Structure summary

• PDB: 4x4t On-Enzyme Refolding Permits Small RNA and tRNA Surveillance by the CCA-Adding Enzyme.
• Resolution: 2.5 Å
• Binding residue (original residue number in PDB): A95 A163 Y165 R224 A228 N229 D291 N292 P295 Q296 R299 R302 G401
• Binding residue (residue number reindexed from 1): A95 A163 Y165 R224 A228 N229 D291 N292 P295 Q296 R299 R302 G401

Enzymatic activity

• Enzyme Commision number: 2.7.7.72: CCA tRNA nucleotidyltransferase.

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0000287 magnesium ion binding
• GO:0003723 RNA binding
• GO:0004810 CCA tRNA nucleotidyltransferase activity
• GO:0005524 ATP binding
• GO:0016779 nucleotidyltransferase activity
• GO:0046872 metal ion binding
• GO:0160016 CCACCA tRNA nucleotidyltransferase activity

Biological Process

• GO:0001680 tRNA 3'-terminal CCA addition
• GO:0008033 tRNA processing
• GO:0031123 RNA 3'-end processing
• GO:0042245 RNA repair
• GO:0106354 tRNA surveillance

External links

• PDB: RCSB:4x4t, PDBe:4x4t, PDBj:4x4t
• PDBsum: 4x4t
• PubMed: 25640237
• UniProt: O28126|CCA_ARCFU CCA-adding enzyme (Gene Name=cca)