Structure of PDB 4irc Chain F Binding Site BS01

Receptor Information

>4irc Chain F (length=342) Species: 83333 (Escherichia coli K-12)

GSRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTANYPAR
KFGVRSAMPTGMALALCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEP
LSLDEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLA
KIASDMNKPNGQFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRT
CGDVQACDLVMLLKRFGKFGRILWERSQGIDERDVNSERLRKSVGVERTM
AEDIHHWSECEAIIERLYPELERRLAKVKPDLLIARQGVKLKFDDFQQTT
QEHVWPRLNKADLIATARKTWDERRGGRGVRLVGLHVTLLDP

Ligand information

>4irc Chain G (length=18)

tctggggtcctaggaccc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4irc A strategically located serine residue is critical for the mutator activity of DNA polymerase IV from Escherichia coli.
• Resolution: 2.67 Å
• Binding residue (original residue number in PDB): R35 R38 V40 S42 A56 M61 K217 R238 R240 K241 S242 V243 G244 V245 E246 R247 T248 R273 F295 R330
• Binding residue (residue number reindexed from 1): R36 R39 V41 S43 A57 M62 K218 R239 R241 K242 S243 V244 G245 V246 E247 R248 T249 R274 F296 R331

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0005515 protein binding
• GO:0008296 3'-5'-DNA exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0000731 DNA synthesis involved in DNA repair
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair
• GO:0006974 DNA damage response
• GO:0009432 SOS response
• GO:0042276 error-prone translesion synthesis
• GO:0070987 error-free translesion synthesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4irc, PDBe:4irc, PDBj:4irc
• PDBsum: 4irc
• PubMed: 23525461
• UniProt: Q47155|DPO4_ECOLI DNA polymerase IV (Gene Name=dinB)