Structure of PDB 3j5s Chain F Binding Site BS01

Receptor Information
>3j5s Chain F (length=234) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDVAVNLGI
DARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDL
ADQIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVA
EAVKNAKAGQVRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAK
PTQAKGVYIKKVSISTTMGAGVAVDQAGLSASVN
Ligand information
>3j5s Chain A (length=174) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ugcccggugccggaagguuaauugaugggguuagcgcaagcgaagcucuu
gaucgaagccccgguaaacggcggaacauugagccuugauguguaggaua
ggugggaggcuuugaaguguggacgccagucugcauggagccgaccuuga
aauaccacccuuuaauguuugaug
.<<<..<<<<<<<..<<<........<<<<<..<<....>>...>>>>>.
.......>>>>>>>>..>>>>>....<<<<<<<<.<<<<...........
.<<<<...<.......<<<<<<<<....>>>>>>>>....>.........
....>>>>...>>>>.>>>>>>>>
Receptor-Ligand Complex Structure
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PDB3j5s EttA regulates translation by binding the ribosomal E site and restricting ribosome-tRNA dynamics.
Resolution7.5 Å
Binding residue
(original residue number in PDB)		M1 K3 R7 M8 R9 V10 I11 R12 T18 T35 A36 K37 F38 S41 D43 R71 R74 K105 K106 M109 L127 V130 L131 G132 P133 R134 G135 L136 M137 R162 R164 D166 K167 N168 I170 H172 T174 K211 S213 S215 T217 M218 A220
Binding residue
(residue number reindexed from 1)		M1 K3 R7 M8 R9 V10 I11 R12 T18 T35 A36 K37 F38 S41 D43 R71 R74 K105 K106 M109 L127 V130 L131 G132 P133 R134 G135 L136 M137 R162 R164 D166 K167 N168 I170 H172 T174 K211 S213 S215 T217 M218 A220
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0005515 protein binding
GO:0019843 rRNA binding
Biological Process
GO:0000027 ribosomal large subunit assembly
GO:0002181 cytoplasmic translation
GO:0006412 translation
GO:0006417 regulation of translation
GO:0045947 negative regulation of translational initiation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005840 ribosome
GO:0015934 large ribosomal subunit
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3j5s, PDBe:3j5s, PDBj:3j5s
PDBsum3j5s
PubMed24389465
UniProtP0A7L0|RL1_ECOLI Large ribosomal subunit protein uL1 (Gene Name=rplA)

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