Structure of PDB 2xx1 Chain F Binding Site BS01

Receptor Information

>2xx1 Chain F (length=335) Species: 85698 (Achromobacter xylosoxidans)

DADKLPHTKVTLVAPPQVHPHEQATKSGPKVVEFTMTIEEKKMVIDDKGT
TLQAMTFNGSMPGPTLVVHEGDYVQLTLVNPATNAMPHSVDFHGATGALG
GAKLTNVNPGEQATLRFKADRSGTFVYHCAPEGMVPWHVVSGMSGTLMVL
PRDGLKDPQGKPLHYDRAYTIGEFDLYIPKGPDGKYKDYATLAESYGDTV
QVMRTLTPSHIVFNGKVGALTGANALTAKVGETVLLIHSQANRDTRPHLI
GGHGDWVWETGKFANPPQRDLETWFIRGGSAGAALYTFKQPGVYAYLNHN
LIEAFELGAAGHIKVEGKWNDDLMKQIKAPAPIPR

Ligand information

• Ligand ID: NO2
• InChI: InChI=1S/HNO2/c2-1-3/h(H,2,3)/p-1
• InChIKey: IOVCWXUNBOPUCH-UHFFFAOYSA-M
• SMILES:
  ACDLabs 10.04
  CACTVS 3.341: [O-]N=O
  OpenEye OEToolkits 1.5.0: N(=O)[O-]
• Formula: N O2
• Name: NITRITE ION
• DrugBank: DB12529
• PDB chain: 2xx1 Chain F Residue 1337

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2xx1 Proton-Coupled Electron Transfer in the Catalytic Cycle of Alcaligenes Xylosoxidans Copper-Dependent Nitrite Reductase.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): H249 I251 H300
• Binding residue (residue number reindexed from 1): H248 I250 H299
• Annotation score: 5

Enzymatic activity

• Catalytic site (original residue number in PDB): H89 D92 H94 H129 C130 H139 M144 H249 E273 T274 H300
• Catalytic site (residue number reindexed from 1): H88 D91 H93 H128 C129 H138 M143 H248 E272 T273 H299
• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).

Gene Ontology

Molecular Function

• GO:0005507 copper ion binding
• GO:0005515 protein binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0050421 nitrite reductase (NO-forming) activity

Biological Process

• GO:0019333 denitrification pathway
• GO:0042128 nitrate assimilation

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:2xx1, PDBe:2xx1, PDBj:2xx1
• PDBsum: 2xx1
• PubMed: 21469743
• UniProt: O68601