Structure of PDB 6z6b Chain EEE Binding Site BS01

Receptor Information

>6z6b Chain EEE (length=2031) Species: 11577 (La Crosse virus)

GMDYQEYQQFLARINTARDACVAKDIDVDLLMARHDYFGRELCKSLNIEY
RNDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSN
ESSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELY
PTIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCP
EFWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKK
DYSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLH
DQKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGK
MMDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQF
MINNDLIDKSEKLKLFKNFCGIGKHSKPKILDFDDANMYLASLTMMEQSK
KILSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDFS
TLMKNILSVSQYNRHNTFRIAMCANNNVFAIVFPSAATVVYSIIVLHKEE
ENIFNPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLFLTTCLLF
KHDNPTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLAISSNVKD
YIAEKFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYLSDYDITQ
KGIKDNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHHVMVDLAK
TILEIECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADTSRHNHLR
NRIENRNNFRRSITTISTFTSSKSCLKIGDFRKEKELQSVKQKKILEVQS
RKMRLANPMFNYEMLRNAMPNYTDYISTKVFDRLYELLDKKVLTDKPVIE
QIMDMMIDHKKFYFTFFNKGQKTSKDREIFVGEYEAKMCMYAVERIAKER
CKLNPDEMISEPGDGKLKVLEQKSEQEIRFLVETTRQKNREIDEAIEALA
SNLGKIEKLSLGKAKGLKMEINADMSKWSAQDVFYKYFWLIALDPILYPQ
EKERILYFMCNYMDKELILPDELLFNLLDQKVAYQNDIIATMTNQLNSNT
VLIKRNWLQGNFNYTSSYVHSCAMSVYKEILKEAITLLDGSILVNSLVHS
DDNQTSITIVQDKMENDKIIDFAMKEFERACLTFGCQANMKKTYVTNCIK
EFVSLFNLYGEPFSIYGRFLLTSVGDCAYIGPYEDLASRISSAQTAIKHG
CPPSLAWVSIAISHWMTSLTYNMLPGQSNDPIDYFPAENRKDIPIELNGV
LDAPLSMISTVGLESGNLYFLIKLLSKYTPVMQKRESVVNQIAEVKNWKV
EDLTDNEIFRLKILRYLVLDAGRSILTPRKFTTAGSLRKLYSFSKYQDRL
SSPGGMVELFTYLLEKPELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQ
NPAQLFIEQILFSHKPVIDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLE
LTNDDIQVIYSYIILNDPMMITIANTHILSIYGSPQRRMGMSCSTMPEFR
SPALVLRAYSKNNPDTEMARDLVHLKEFVENTNLEEKMKVRIAMNEAEKG
QRDIVFELKEMTRFYQVCYEYVKSTEHKIKVFILPAKSYTTTDFCSLMQG
NLIKDKEWYTVHYLKQILSGGHKAINATSEQNIAFECFKLITHFADSFID
SLSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLFRTGDLRQ
ADLDKYDAMKRILIRSLDYLNNDIFSLSRIKVGLDEFATIKKAHFSKMVS
FEGPPIKTGLLDLTELMKSQDLLNLNYDNIRNSNLISFSKLICCEGSDNI
NDGLEFLSDDPMNFTEGEAIHSTPIFNIYYSKRGERHMTYRNAIKLLIER
ETKIFEEAFTFSENGFISPENLGCLEAVVSLIKLLKTNEWSTVIDKCIHI
CLIKNGMDHMYHSFDVPKCFMGNPITRDINWVMFREFINSLPGTDIPPWN
VMTENFKKKCIALINSKFETQRDFSEFTKLM

Ligand information

>6z6b Chain BBB (length=10)

aguagugugc

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Receptor-Ligand Complex Structure

Structure summary

• PDB: 6z6b Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
• Resolution: 3.961 Å
• Binding residue (original residue number in PDB): Q291 K302 H306 C419 G420 K423 H424 P440 R592 A593 R595 R600 T642 K643 Y677 K679 E758 H760 H761 V764 K768 K1117 V1118 Y1120 D1123
• Binding residue (residue number reindexed from 1): Q292 K303 H307 C420 G421 K424 H425 P428 R574 A575 R577 R582 T624 K625 Y659 K661 E740 H742 H743 V746 K750 K1081 V1082 Y1084 D1087

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:6z6b, PDBe:6z6b, PDBj:6z6b
• PDBsum: 6z6b
• PubMed: 32681014
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)