Structure of PDB 7a5g Chain E3 Binding Site BS01

Receptor Information

>7a5g Chain E3 (length=300) Species: 9606 (Homo sapiens)

SGTWWDEHLSEENVPFIKQLVSDEDKAQLASKLCPLKDEPWPIHPWEPGS
FRVGLIALKLGMMPLWTKDGQKHVVTLLQVQDCHVLKYTSKGKMATLSVG
GKTVSRFRKATSILEFYRELGLPPKQTVKIFNITDNAAIKPGTPLYAAHF
RPGQYVDVTAKTIGKGFQGVMKRWGFKGQPATHGQTKTHRRPGAVATGDI
GRVWPGTKMPGKMGNIYRTEYGLKVWRINTKHNIIYVNGSVPGHKNCLVK
VKDSKLPAYKDLGKNLPFPTYFPDGDEEELPEDLYDENVCQPGAPSITFA

Ligand information

>7a5g Chain A3 (length=1503)

gcuaaaccuagccccaaacccacuccaccuuacuaccagcaaccuuagcc
aaaccauuuacauaaaguauaggcgauagaaauuguggcgcaauagauau
aguaccgcaagggaaagaugaaaaauuauaaccaagcauaauauagcaag
gacuaaccccuauaccuucugcauaaugaauuaacuagaaauaacuuugc
aaggagagccaaagcuaagacccccgaaaccagacgagcuaccuaagaac
agcuaagagcacacccgucuauguagcaaaauagugggaagauuuauagg
uagaggcgacaaaccuaccgagccuggugauagcugguuguccaagauag
aaucuuaguucaacuuuaaauuugcccacagaaccaaauccccuuguaaa
uuuaacuguuaguccaaagaggaacagcucuuuggacacuaggaaaaaac
cuuguagagagaguaaaaaauuuaacacccauaguaggccuaaaagcagc
caccaauuaagaaagcguucaagcucaacaccaaaaaucccaaacauaua
acugaacuccucacacccaauuggaccaaucuaucacccuauagaagaac
uaauguuaguauaaguaacaugaaaacauucuccuccgcauaagccugcg
ucagaucacacugacaauuaacagcccaauaucuacaaucaaccaacaag
ucauuauuacccucacugucaacccaacacaggcaugcucauaaggaaag
guuaaaaaaaguaaaaggaacucggcaaaucuuaccccgccuguuuacca
aaaacaucaccucuagcaucaccaguauuagaggcaccgccugcccagug
acacauguuuaacggccgcgguuaaccgugcaaagguagcauaaucacuu
guuccuuaaauagggaccuguaugaauggcuccacgaggguucagcuguc
ucuuacuuuuaaccagugaaauugaccugcccgugaagaggcgggcauaa
cacagcaagacgagaagacccuauggagcuuuaauuuauuaaugcaaaca
guaccuaacaaacccacagguccuaaacuaccaaaccugcauuaaaaauu
ucgguuggggcgaccucggagcagaacccaaccuccgagcaguacaugcu
aagacuucaccagucaaagcgaaccucaauugauccaauaacuugaccaa
cggaacaaguuacccuagggauaacagcgcaauccuauucuagaguccau
aucaacaauaggguuuacgaccucgauguuggaucaggacaucccgaugg
ugcagccgcuauuaaagguucguuuguucaacgauuaaaguccuacguga
ucugaguucagaccggaguaauccaggucgguuucuaucuacuuuauucc
ucccuguacgaaaggacaagagaaauaaggccuacuucacaaagcgccuu
cccccguaaaugauaucaucucaacuuagauacccacacccaagaacagg
guu

<<<.......<<<<....................................
<......>...........<<.....>>........>>.>>.........
....<<....>>......................>>>...........<<
<.......>>>...<.<........>.>.<<<<<<.........<<<.<<
..<<....>>...>>.>>>...<<.<..<<<<<.<.....<<<<<.<<<.
.<<<...>>>...<<<<.<<<<........>>>>>>>>....>>>.>>>>
>...<<.......>>......>.>>>>...>..>.>>..<<<<<<....<
<<<....>>>><<<.<<<<<<<<<<.....<<......>>.....>>>>>
>>>>>..>>>.<<<<<<<<<<......>>>>>>>>>>...<<<......>
>>....<<<.<<<...<..............<....<<<.........>>
>....>.......<<<......>>>.........................
..>...>>>>>>........>>>>>>...<<<<<......>>>>>.....
....>>>>>><......<<..<<<....>>><<<<..<<<<........<
<<<<......>>>>>......<<<...<<<<...................
....>>>>.......>>>.....<<......>>.>>>>.....>>>>..<
<<<...<<<<<<<<.<<.........<......<<<<<<..<<<......
...>>>...<<<<<<.............>>>>>>....<<<.<<<..<<<
<<....>>>..>>>>><<<<<....>>>>>........<...........
<<<<<<.....>>>>>>.....>....>>>....>>.>>>>.....>..>
>.>>>>>>>>>>>>.>>.....>.....<<<<<........>>>>>....
...<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<<<....
.....................................>>>>>>>>>>>>>
..<<<<.....>>>><<<<<<...........>>>>>>..<<.....<<<
..<<<<.....>>>>..>>>....>>.........<<<....>>>.....
.......>>>>..>>>>>>.......<<<..<.<<<<<<...<<......
.>>...>>>>>>...>.>>.>.......<<<<<.<<<<<<..<<.<<<<<
<......>>>>>>...>>.<<<<<.....>>>>>.....>>>>>..>...
>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>.>>..<<<.<
<<.................>>>.>>><<<<<...<<<....>>>.>>>>>
......<<<................>>>.........<<<<.......>>
>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7a5g Elongational stalling activates mitoribosome-associated quality control.
• Resolution: 4.33 Å
• Binding residue (original residue number in PDB): W48 V65 K103 M106 K116 V118 V152 S153 R154 F155 R156 I161 V176 K177 I178 I211 G212 K213 G214 F215 G217 K220 R221 F224 K225 P228 A229 T230 H231 G232 Q233 T234 K235 T236 H237 R238 R239 G241 A242 V243 A244 T245 G246 D247 I248 R250 V251 W252 P253 G254 M257 K260 G262 Y265 Y269 S288 V289 P290 G291 H292 K293 N294 K298 K300 K303 L304
• Binding residue (residue number reindexed from 1): W4 V21 K59 M62 K72 V74 V104 S105 R106 F107 R108 I113 V128 K129 I130 I163 G164 K165 G166 F167 G169 K172 R173 F176 K177 P180 A181 T182 H183 G184 Q185 T186 K187 T188 H189 R190 R191 G193 A194 V195 A196 T197 G198 D199 I200 R202 V203 W204 P205 G206 M209 K212 G214 Y217 Y221 S240 V241 P242 G243 H244 K245 N246 K250 K252 K255 L256

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation
• GO:0032543 mitochondrial translation

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005762 mitochondrial large ribosomal subunit
• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7a5g, PDBe:7a5g, PDBj:7a5g
• PDBsum: 7a5g
• PubMed: 33243891
• UniProt: P09001|RM03_HUMAN Large ribosomal subunit protein uL3m (Gene Name=MRPL3)