Structure of PDB 8uu9 Chain E Binding Site BS01

Receptor Information

>8uu9 Chain E (length=204) Species: 1642 (Listeria innocua)

KLSLLKQDGTNAGEITLNDTVFGIEPNEKVVVDVILSQRASLRQGTHKVK
NRSEVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYAYKLP
KKVRRLAIKSILSSKVNEEKLVVLEGLTFDAPKTKEFAAFLKNISVDTKA
LIVVAGESENVELSARNLQGITVIPAESISVLEVAKHDKLIITKAAVEKV
EEVL

Ligand information

>8uu9 Chain A (length=2899)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaaga
aggacgggacuaacaccgauaugcuuuggggagcuguacguaagcguuga
uccagagauuuccgaaugggggaacccacuaucuuuagucggauaguauc
cuuacgugaauacauagcgugaggaaggcagacccagggaacugaaacau
cuaaguaccuggaggaagagaaagaaaaaucgauuuccugaguagcggcg
agcgaaacggaaagagcccaaaccaagaagcuugcuucuugggguuguag
gacacucuauacggaguuacaaaagaaaguuauaaaugaagcggucugga
aaggcccgccaaagacgguaacagcccgguaguugaaauggcuuucccuc
cagaguggauccugaguacgacggaacacgugaaauuccgucggaauccg
ggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaacagu
uccugaaaccgugugccuacaaguaguuagagcccguuaaugggugauag
cgugccuuuuguagaaugaaccggcgaguuacgauuuguugcaagguuaa
gcggaaaaagcggagccguagcgaaagcgagucugaauagggcgcauaag
uaacaggucguagacccgaaaccaggugaucuacccauguccaggaugaa
gguaagguaauacuuacuggagguccgaacccacgcacguugaaaagugc
ggggaugagguguggguagcggagaaauuccaaucgaacuuggagauagc
ugguucucuccgaaauagcuuuagggcuagccucgagguaaagagucaug
gagguagagcacuguuuggacuaggggaccgaauucagauaaacuccgaa
ugccauguacuuauacucgggagucagacugcgagugauaagauccguag
ucgaaagggaaacagcccagaccaccaguuaagguccccaaauauauguu
aaguggaaaaggaugugggguugcuuagacaaccaggauguuggcuuaga
agcagccaccauugaaagagugcguaauagcucacuggucgagugacccc
gcgccgaaaauguaccggggcuaaacauauuaccgaaacuguggaugaac
cuagguucgugguaggagagcguucuaagggcggugaagucagaccggaa
ggacugguggagcgcuuagaagugagaaugccgguaugaguagcgaaaga
agggugagaaucccuuccaccgaauaucuaagguuuccugaggaaggcuc
guccgcucaggguuagucgggaccuaagccgaggccgauaggcguaggcg
auggacaacagguagagauuccuguaccagugcuaauuguuuaaccgaug
gggugacacagaaggauagggaaucgcacgaauggaaaugugcguccaag
cagugagugugagaaguaggcaaauccgcuucuugcgaagcaugagcugu
gauggggaagacggaaguuccugauuucacgcugucaagaaaagccucua
ggaagaguaguacugcccguaccgcaaaccgacacagguagaugaggaga
gaauccuaaggugagcgagagaacucucguuaaggaacucggcaaaauga
ccccguaacuucgggagaaggggugcucuauuagggugcaagcccgagag
agccgcagugaauaggcccaggcgacuguuuagcaaaaacacaggucucu
gcaaaaccguaaggugacguauaggggcugacgccugcccggugcuggaa
gguuaagaggagugcuuagcuucggcgaagguacgaauugaagccccagu
aaacggcggccguaacuauaacgguccuaagguagcgaaauuccuugucg
gguaaguuccgacccgcacgaaaggcgcaacgaucugggcacugucucaa
cgagagacucggugaaauuauaguaccugugaagaugcagguuacccgcg
acaggacggaaagaccccguggagcuuuacugcaaccugauauggaaugu
uuguaccgcuuguacaggauagguaggagccgaagagacgugugcgcuag
cauacgaggaggcaauggugggauacuacccuggcuguaugaccauucua
acccgccacgcuaagcgcguggggagacagugucaggugggcaguuugac
uggggcggucgccuccuaaagaguaacggaggcgcccaaagguucccuca
gaauggauggaaaucauucgcagaguguaaaggcacaagggagcuugacu
gcgagacugacaagucgagcagggacgaaagucgggcuuagugauccggu
gguuccgcauggaagggccaucgcucaacggauaaaagcuaccccgggga
uaacaggcuuaucucccccaagaguccacaucgacggggagguuuggcac
cucgaugucggcucgucgcauccuggggcuguagucggucccaaggguug
ggcuguucgcccauuaaagcggcacgcgagcuggguucagaacgucguga
gacaguucggucccuauccgucgcgggcgcaggaaauuugagaggagcug
uccuuaguacgagaggaccgggauggacacaccgcugguguaccaguugu
uccgccaggagcaucgcuggguagcuauguguggcagggauaaacgcuga
aagcaucuaagcgugaagccccccucaagaugagauuucccauuucuucg
gaaaguaagaucccugaaagaugaucagguagauagguuuggaguggaag
uguagcgauacauggagcggacaaauacuaaucgaucgaggacuuaacc

<<<<<<......<<<<<<<<<<<....{[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>....<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<
<<<<<<<<....<<<<........<<<<<<<<<.<<<.....<<<<....
..>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>.>>>
>>>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]...........
....<<....>>....]].........<<<<....>>>>...........
..}....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...
<<.......>>...>>>...<<....>>..<<<<.....>>>>......>
>>>>>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<....
<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>
>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>
.>>..<<<<<<....<<<<<....>>>>><<<<<<<<.....<<<<<<<<
<.....<<<...<<<<<<<<...<<...>>...>>>>>>>>..>>.>...
..>>>>>.>>>>...>>>>>>.>>..<<<<<<<.<<......>>.>>>>>
>>....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.
..........<<..<<<<<<<<<<.......<<<<<.<<...<<<<....
...>>>>...>>.........<<......>>...>>>>>...>>>>>>>>
>>.>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<
<..>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<<.<<....
>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......<<....<<
<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<...<<...
..>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>.
..>>...<<<<<........>>>>>))<<<<<<<<.<<......<<...<
<<<<...<<<<..<<<..<<<<<.<<<<<...(((....>>>>>.<<..<
<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>..>>>>>
...>>............>>>>>...>>>...>>>>........>>>>>..
>>..>>.>>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<..
...>>>>..>>>>..<<<<....<<<<<<<<.<<.........<.....<
<<<<....<<..>>.....>>>>><<<<<....<<<<....>>>>...>>
>>>............<<<<<<<<<..<<<.........>>>..<<<<<<<
.....<<<....>>>.......>>>>>>>..<<<<.<<<..<<<<<<<..
<<<<..<<...<<<<<<.<<<..>>>..>>>>>>...>>..>>>>>>>>>
..>>>>><<<<<<.......>>>>>>........<...........<<<<
<<.....>>>>>>.....>....>>>>...>>.>>>>>>>..>..>>.>>
>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<<
<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<<<.<<<<<.
<<<<<<.<<<.<.........<<<<<..<<<.......<<<<<<<....>
>>>>>>....>>>............>>>>>>.>>>.>>>>>>.>>>>>..
..<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<
<.....>>>><<<<<<...........>>>>>>..<<...<<<<<<<...
<<<<<.<......>>>>>>.....<<.[.(((>>....>>>>>>>...<<
<<..<<<<....>>>>..>>>>.<<<....>>>.>>)))]......<<<<
<<<.((..>>>>..))>>>>>>>.............>>>>..]]>>>>..
.....<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>>>.>
.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<
<<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....
>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<
<<<<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<
<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<.<..<<<<<..
.........>>>>>...>>>>.>>>>.......>>>>>>>..<<<<<..>
>>>>....<<<<<<<<<.......>>>>>....<<<<<<<<..<<....<
<<<<....>>>>>...>>...>>>>>.>>>...>>>>....>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8uu9 Mechanistic insights into the alternative ribosome recycling by HflXr.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): R41 R45 Q46 T48 K50 V51 K52 R54 S55 V57 R58 R62 K63 Q67 K68 G71 R72 R74 Q75 G76 R79 Q82 R84 V88 V89 F90 R95 Y99 K104 V105 R106 K135 T136 L165 R168 N169 L170 Q171 E179 S180 S182
• Binding residue (residue number reindexed from 1): R39 R43 Q44 T46 K48 V49 K50 R52 S53 V55 R56 R60 K61 Q65 K66 G69 R70 R72 Q73 G74 R77 Q80 R82 V86 V87 F88 R93 Y97 K102 V103 R104 K133 T134 L163 R166 N167 L168 Q169 E177 S178 S180

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8uu9, PDBe:8uu9, PDBj:8uu9
• PDBsum: 8uu9
• PubMed: 38407413
• UniProt: A0A0U4VSH7