Structure of PDB 8pzy Chain E Binding Site BS01

Receptor Information

>8pzy Chain E (length=499) Species: 652611 (Pseudomonas aeruginosa PA14)

FQGGMEFLVKSVRPETLKTATLVLAVGEGRKLGASAKAVDDATGGAISAV
LKRGDLAGKVGQTLLLQSLPNLKAERVLLVGAGKERELGDRQYRKLASAV
LSTLKGLAGADAALALGDLAVKGRGAHAKARLLVETLADGLYVFDRYKSQ
KAEPLKLKKLTLLADKADSAAVEQGSKEAQAIANGMALTRDLGNLPPNVC
HPTFLGEQAKGLAKEFKSLKVEVLDEKKLRELGMGSFLAVAQGSDQPPRL
IILQYNGAKKDQAPHVLVGKGITFDTGGISLKPGLGMDEMKFDMCGAASV
FGTFRAVLELQLPINLVGLLACAENMPSGGATRPGDIVTTMSGQTVEILN
TDAEGRLVLCDALTYAERFKPQSVIDIATLTGACIVALGSNTSGLMGNNE
ALVRQLLKAGEFADDRAWQLPLFDEYQEQLDSPFADIANIGGPKAGTITA
GCFLSRFAKKYHWAHLDIAGTAWISGGKDKGATGRPVPLLTQYLLERAK

Ligand information

• Ligand ID: BCT
• InChI: InChI=1S/CH2O3/c2-1(3)4/h(H2,2,3,4)/p-1
• InChIKey: BVKZGUZCCUSVTD-UHFFFAOYSA-M
• SMILES:
  OpenEye OEToolkits 1.5.0: C(=O)(O)[O-]
  CACTVS 3.341: OC([O-])=O
  ACDLabs 10.04: [O-]C(=O)O
• Formula: C H O3
• Name: BICARBONATE ION
• PDB chain: 8pzy Chain E Residue 602

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8pzy Unveiling the Catalytic Mechanism of a Processive Metalloaminopeptidase.
• Resolution: 1.97 Å
• Binding residue (original residue number in PDB): A370 G372 R373 L397
• Binding residue (residue number reindexed from 1): A353 G355 R356 L380
• Annotation score: 4

Enzymatic activity

• Enzyme Commision number: 3.4.11.1: leucyl aminopeptidase.
  3.4.11.10: bacterial leucyl aminopeptidase.

Gene Ontology

Molecular Function

• GO:0004177 aminopeptidase activity
• GO:0008235 metalloexopeptidase activity
• GO:0030145 manganese ion binding
• GO:0046872 metal ion binding
• GO:0070006 metalloaminopeptidase activity

Biological Process

• GO:0006508 proteolysis
• GO:0019538 protein metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8pzy, PDBe:8pzy, PDBj:8pzy
• PDBsum: 8pzy
• PubMed: 37924287
• UniProt: Q02RY8|AMPA_PSEAB Probable cytosol aminopeptidase (Gene Name=pepA)