Structure of PDB 8i7j Chain E Binding Site BS01

Receptor Information
>8i7j Chain E (length=260) Species: 284590 (Kluyveromyces lactis NRRL Y-1140) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
ARGPKKHLKRLAAPHHWMLDKLSGCYAPRPSAGPHKLRESLPLIVFLRNR
LKYALNGREVKAILMQRHVKVDGKVRTDTTFPAGFMDVITLEATNENFRL
VYDVKGRFAVHRITDEEASYKLAKVKKVQLGKKGIPYVVTHDGRTIRYPD
PNIKVNDTVKVDLATGTITDFIKFDTGKLVYVTGGRNLGRVGTIVHRERH
EGGFDLVHIKDSLENTFVTRLNNVFVIGEPGRPWISLPKGKGIKLTISEE
RDRRRAQHGL
Ligand information
>8i7j Chain 2 (length=1780) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacauggauaucug
ugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaagag
auguauuuauuagauaaaaaaucaaugucuucggacuccuugaugauuca
uaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaaa
uuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuuc
aacggguaacggggaauaaggguucgauuccggagagggagccugagaaa
cggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccua
auucagggagguagugacaauaaauaacgauacagggcccauucgggucu
uguaauuggaaugaguacaauguaaauaccuuaacgaggaacaacuggag
ggcaagucuggugccagcagccgcgguaauuccagcuccaguagcguaua
uuaaaguuguugcaguuaaaaagcucguaguugaacuuugggucugguug
uccggucgguuuuucaaccggaucuuuccuucuggcuaaccuguacuccu
ugugggugcaggcgaaccaggacuuuuacuuugaaaaaauuagaguguuc
aaagcaggcgaaagcucgaauauauuagcauggaauaauggaauaggacg
uuugguucuauuuuguugguuucuaggaccaucguaaugauuaauaggga
cggucgggggcaucaguauucaauugucagaggugaaauucuuggauuua
uugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaauc
aagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucuua
accauaaacuaugccgacuagggaucgggugguguuuuucuuaugaccca
cucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguaugg
ucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggagug
gagccugcggcuuaauuugacucaacacggggaaacucaccagguccaga
cacaauaaggauugacagauugagagcucuuucuugauuuuguggguggu
ggugcauggccguucuuaguugguggagugauuugucugcuuaauugcga
uaacgaacgagaccuuaaccuacuaaauaggguugcuggcacuugccggu
ugacucuucuuagagggacuaucgguuucaagccgauggaaguuugaggc
aauaacaggucugugaugcccuuagacguucugggccgcacgcgcgcuac
acugacggagccagcgaguacaaccuuggccgagaggucuggguaaucuu
gugaaacuccgucgugcuggggauagagcauuguaauuauugcucuucaa
cgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacguccc
ugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuuagug
aggccucaggauuugcuuagagaagggggcaacuccaucucagagcgaag
aaucuggucaaacuuggucauuuagaggaacuaaaagucguaacaagguu
uccguaggugaaccugcggaaggaucauua
...<<<<<...((((>>>>><<<..<<<<<<...<.<..........<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<................<<.....<<.
<<.......>>.>>......>>........<<<................>
>><...<<<<<<.............................<......>.
>>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>>
>..>>>.<<<....<<<.....<<<<<<<.......>>>>>>>.>>>...
...>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<........
..>>>.>>................>>>>>>.........<<<....<<<.
....>>>..>>>.......>.>.....<<<..<<<<............>>
>>...>>>......<<..<...........>..>>.........<<<<<(
(......<<<<.....<<..))>>.......>>>>.>>>>>..>>>>>>.
>>>.........<<<((.....<.<<...<<<.<<....<....<<<<<<
..............>>>>>>....>....<....................
.....................>...<<<<<.<<.......<<...<....
...>..<<<....>>>....>>......>>.>>..>>>.........<<<
......<.................>.......>>>.....>>....<<<<
<<.<<...<<<<..<<<.<<<<<<.....<<.......>>.........>
>>>>>.>>>.......<<....>>...>>>>...>>>>>.>>>...>>>.
..>>.>....<<<<<<..<.....<<<<.........>>>>....>>>>>
>>..........<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>
>>>>>..>>>...<<..))>>...>>.....>>>.>>>.<<<......<<
<......>>>....>>>..))))..<<<<<.<<<<<<<..<<..<<<<<.
.<<<.<<<<<<......<<........>>..........<<<<<......
<<<<<........<<.<<<........>>>.>>......>>>>>...<<.
<<<......<<<<<....<<<.<.<<......>>...<<<......>>>.
...>.>>>....<<<<<.<<..<<<<..<<<<<<<<<<<<....>>>>>>
.>>>>>>..>>>>.>>....<<<<<<.....>>>>>>.......>>>>>.
...>>>.>>........>>>>>.......>>>>>...>>.>>>>.>>>..
...<<<<<<<......<<.....<<<.<<<<....>>>>.>>>....>>.
......>>>>>>>......<.....<<<<<..........>>>>>.....
>.....>>>>>......<<<<<<<.......>>>>>>>.......>>...
>>>>>>>>>>.>>....<..<<......<<.<<....<<<<<<<<.<<<.
.<<<<..<.....<<<...<....................>...>>>...
..>..>>>>..>>>.>>>>>>>>...>>.>>.......>>...>.....<
<<<<<<<<....>>>>>>>>>.........
Receptor-Ligand Complex Structure
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PDB8i7j Yeast eukaryotic initiation factor 4B remodels the mRNA entry site on the small ribosomal subunit
Resolution4.6 Å
Binding residue
(original residue number in PDB)
A2 R3 K6 K7 H8 K10 L12 A13 H16 M19 D21 K22 L23 Y27 P29 A33 G34 P35 H36 K37 L38 R49 N57 G58 M66 K75 R77 T81 K106 Q130 L131 G132 K133 K134 G135 V140 H142 G144 R145 T146 K155 G185 G186 R187 R221 R255
Binding residue
(residue number reindexed from 1)
A1 R2 K5 K6 H7 K9 L11 A12 H15 M18 D20 K21 L22 Y26 P28 A32 G33 P34 H35 K36 L37 R48 N56 G57 M65 K74 R76 T80 K105 Q129 L130 G131 K132 K133 G134 V139 H141 G143 R144 T145 K154 G184 G185 R186 R220 R254
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0019843 rRNA binding
Biological Process
GO:0006412 translation
Cellular Component
GO:0005840 ribosome
GO:0022627 cytosolic small ribosomal subunit
GO:0032991 protein-containing complex
GO:0043232 intracellular non-membrane-bounded organelle
GO:1990904 ribonucleoprotein complex

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Molecular Function

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Biological Process

View graph for
Cellular Component
External links
PDB RCSB:8i7j, PDBe:8i7j, PDBj:8i7j
PDBsum8i7j
PubMed
UniProtQ6CWJ2

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