Structure of PDB 8fn2 Chain E Binding Site BS01

Receptor Information

>8fn2 Chain E (length=206) Species: 224326 (Borreliella burgdorferi B31)

MLGLIGKKVGMTQIFQKNGIVVPVTVIEFQPNYIIGKKTVDRDGYSALIA
GSVDLKSSKVSKPIKGQYKSLKDIEPKRYVIELKGLDGYDAGDEIKVDVF
KSVKYVDVTGTTKGKGFQGAMKRHNFSGGPSSHGSKFHRHLGGTGQATTP
ARTFKGTKMAGRMAGNQQTIQNLEVVLIDEEKRALLVKGAVPGAKGSFVV
VKKSKK

Ligand information

>8fn2 Chain A (length=2762)

uggucaaaguaauaagagucuauggugaaugccuaggagcuuuaaggcga
agaaggucgugguaagcugcgaaaagcuugggggagaagcaaacauuuau
ugaucccaagauuaccgaauggaguaauccagcuagcaagaugcuagcua
ucuauuauuuaaauaauagaggcgauaccaggggaagugaaccaucuaag
uacccugaggaaaagaaaucaaagagauucccuuaguaguggcgagcgaa
aagggaguagcccaaacuuuaaaugugucaagcugcagagcguugcauuu
augggguuguaggacguuuaggcuuagucuguaauaagcaaaaaaguuac
aaaauauuuauauagaagaauaaucuggaaaguuuaaccaaagaagguga
uaguccuguaauuuaaauguaaauaucuuuuuaaaauguuccugaguagg
acgaggcacgagaaaccuuguuugaagcuggggagaccacucuccaaggc
uaaauacuagaaagcuaccgauagagaagaguaccgugagggaaagguga
aaagaaccccgggaggggagugaaauagaacugaaaccguagacuuacaa
gcagucaaagccguaauuuauugcggugauggcgugccuuuugcauaaug
aaccugcgaguuaucaugucuagcaagauuaaagcauagaagugcuggag
ucgaagcgaaagcgagucuuaaaagggcgauuuaguuagaugugguagac
ccgaagccgagugaucuauuuauggccaggcugaagcuuggguaaaacca
aguggagggccgaacucuagucuguuuaaaaaggcagggaugagcuguga
auaggagugaaaggcuaaacaaacucggagauagcugguucuccccgaaa
uggauuuaaguucagccuuauuuuaguuuaauagagguagagcacuaauu
gagcuagggccugucaaaggguaccaaacucaguuaaacuccgaaugcua
uuaaaugaugaauaggagugagacuaugggcgauaagguucauagucgag
agggaaacaacccagaccaacagcuaaggucucaaaaauguguuaagugg
aaaaggagguuuagguacguaaacagccaggagguuggcuuagaagcagc
cauaccuuuaaagagugcguaauagcucacuggucgaguacuuaagcgcc
gauaauguaacggggcuaaacacauuaccgaagcuuuggaucuuaacgaa
aguuaagaugguaggggagcguucuguaagccagagaaguuaagcuggaa
aguuugauggagguaucagaagugagaaugcagguaugaguaacgaaaaa
augggugagauucccauucgccgaaaaccuaagguuuccuggguaaaggu
cgucuucccaggguuagucggccccuaaggcaaagcugaaaaguguaguc
gaugggaaacggguuaauauucccguaccucuuauaguuucgauggagug
acgcaugagguuaacuacugcuaggcgaugguuguccuaguuuaagcauu
aaggcgaugaucuuaauaggaaaauccguuaagagagcuaagaugugaug
augagugcuauuuagguagcaugaaauguagguagucaaggugccaagaa
auagcuucuaagguuaggcuauaagggaccguaccgcaaaccgacacagg
uaggugggaugaaaauucuaaggcgcgcgagagaauccacguuaaggaac
ucugcaaaauacguacguaacuucgggauaaguacgaccuaagcaauuag
guagcauaaaaaugguccaaacgacuguuuaccaaaaacacaggucucug
caaaucuguaaagagaaguauagggacugacaccugcccggugcuaguaa
acggcuaagguagccggguaaguuccgacgaaugguguaacgauuuggac
gcugucucaacguggagcucggugaaauugaaguaucggugaagaugccg
auuacuugugguuagacggaaagaccccgugaaccuuuacuauagcuugg
uauugagauuugauuaauuaauuagguuucuaacuuauagaaauaugagg
agagugccagguggguaguuugacuggggcggucgccuccuaaagaguaa
cggaggugcgcaaagguuaccuuagagugguuggaaaucacucuguaagu
guaaaggcauaagguagcuuaacuguaagacugacaagucgaacagauac
gaaaguaggucuuagugaucuggcgguggcaaguggaagcgccgucacuu
aacgaauaaaagguacuccggggauaacaggcuuauccuucccaagaguu
cacaucgacggaaggguuuggcaccucgaugucggcucaucgcauccuag
ggcuggagcagguccuaaggguauggcuguucgccauuuaaagcgguacg
cgagcuggguucagaacgucgugagacaguuuggucccuaucugccacaa
gcguuggauauuugagaggagcuaucuuuaguacgagaggaccgagaugg
acgaaccucuagugugccaguuauccugccaaggguaagugcuggguagc
uacguucggaaaggauaaccgcugaaagcaucuaagugggaagccuuccu
caagaugagauauccuuuaaggguccuggaagaauaccagguugauaggu
uagaaguguaaguauagcaauauauuaagcugacuaauacuaauuacccg
uaucuuuggcca

.<<<<<<<<......<<<<<<<<<<<.....<<..<<<<<<<<.<<<<..
....<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>..
...>>>>>>>...>>>....<<<....>>><<<<<<<.....>>>>>>>.
<<<<<<<.<..>.>>>>>>>.>>....<<<<<<.....<<....>>....
..>>>>>.>.........<<...>>..<<<<<<.....<<.....>>...
.>>>>>>..<<<....<<.<<<<<<<.......<<.<<<.....>>>>>>
>.>>......[<<<<.[....<<<<<.>>>.>>.>>>>>.<<<<......
..<.<<<<.<<<<.<<....<<<.<......>.>>>((......<<<...
..))>>>....>>...>>>>>>.>>>.>>>>...]..>>>>.].....<<
<<<<<<.........>>>>>>>>......<<<<<..((((>>>>>..>>>
......>>..>>>>>>.>>..............<<....>>....>>...
......<<<<....>>>>.................>>>>>>>>>>>....
...<<<<..<<<<<<<....>>>>>>>..>>>>...<.<........>.>
.<<<<<<....<<<<<<<<<<<<<..<<<...<<<<<....>>>>>...>
>>...<<....>>..<<<<.....>>>>......>>>>>>>>>>>>>..<
<.<..<<<<<<<.....<<<<<<<<<..<<<....<<<<<<......>>>
>>>.....>>>...<<<<.<<<<........>>>>>>>>....>>>>>>>
>>...<<<.....>>>......>>>>>>...>..>.>>..<<<<<<....
<<<<<....>>>>><<<<<<<<<..<<<<<<<<<.....<<<...<<<<<
<<<...<<<<<<......>>>>.>>...>>>>>>>>..>>.>.....>>>
>>>>>>...>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>...
.<<<......>>>...<<<<.<<<...<<<((....<<<<<<<.......
....<<..<<<<<<<<<........<<<<<.<<...<<<.........>>
>....>>.........<<......>>...>>>>>....>>>>>>>>>.>>
............)).....>>>>>>>>>>...>>>>>>><<<<<<<<...
.>>>>>>>>....>>>>>>..<<<<<...<<<......<<<<<<<<....
>>>>>>>>...>>>..>>>>>........>>>>>><......<<.....<
<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<<<<...<<..
...>>.>>>>>>....((<<<.<<<...<<<...<<<....>>>...>>>
...>>>..<<<<<........>>>>>)).<<<<<<<<<.<<...<<<<<.
..<<<<..<<<...<<<<.<<<<<<...[[[[...>>>>>><<..<<<<<
..<<<........<<<............>>>.....>>>..>>>>>...>
>...<<<<<<<....>>>>>>>.....>>>>...>>>...>>>>......
...>>>>>...>>....>>>>>>>>>..>>>.>>>.....<<......>>
>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<<.<<....
.....<.....<<<<<...............>>>>><<<<<<....>>>>
>>............<<<<<<<<<..<<<.........>>>..<<<<<<<.
....<<<....>>>.......>>>>>>>.<<<<<..<<..<<<<..>>..
>>>>........<.<<<.......>>>.>....>>>>>..>>.>>>>>>>
..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>
>>..<<<<<<<<.<<<.<<......<<<<.<<<<<<..<<<<<.<<<<<<
<<<.<<<<<<<<<<<<..>>>>>>>>>>>>...<<<<<<....>>>>>>.
...>>>>>>>>>..>>>>>..<<<<.....>>>><<<<<<..........
.>>>>>>..<<...<<<<<<<.<<<<<<<<.......>>>>>>>>...<<
.[.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.<<<
....>>>.>>)))]......<<<<<<<.<<..))))..>>>>>>>>>...
..........>>>>..>>>>>>.......<<<<.<.<<<<<<...<<...
....>>...>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<
<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>
...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>>>>
>....<<<<<<<...<<<<..<<<<<<<...............>>>>>>>
.<<<<<...<<<<...<<<<<<<<<<<]]]]>>>>>>...>>>>>...>>
>>.>>>>>..<<<<....<<<<<...........>>>>>....>>>>>>>
>.......>>>>>>>.....<<<<<<<<<.......>>>>>....<<<<<
<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>.
...>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fn2 The structure of a hibernating ribosome in a Lyme disease pathogen.
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): M11 T12 Q13 P23 R42 D43 Y45 K56 K59 S61 K62 P63 G66 Q67 K69 V80 I81 E82 K113 G114 G116 F117 G119 K122 R123 S131 S132 H133 G134 K136 F137 H138 R139 L141 G142 G143 Q146 A147 T148 T149 P150 A151 R152 T153 K155 G156 T157 M159 A160 R162 Q167 Q168 Q171 N172 K188 A190 P192 A194 G196 K206
• Binding residue (residue number reindexed from 1): M11 T12 Q13 P23 R42 D43 Y45 K56 K59 S61 K62 P63 G66 Q67 K69 V80 I81 E82 K113 G114 G116 F117 G119 K122 R123 S131 S132 H133 G134 K136 F137 H138 R139 L141 G142 G143 Q146 A147 T148 T149 P150 A151 R152 T153 K155 G156 T157 M159 A160 R162 Q167 Q168 Q171 N172 K188 A190 P192 A194 G196 K206

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fn2, PDBe:8fn2, PDBj:8fn2
• PDBsum: 8fn2
• PubMed: 37907464
• UniProt: P94267|RL3_BORBU Large ribosomal subunit protein uL3 (Gene Name=rplC)