Structure of PDB 7zpl Chain E Binding Site BS01

Receptor Information

>7zpl Chain E (length=618) Species: 1318616 (Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9)))

MDVNPTLLFLKVPVQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHKYS
EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP
GIFENSCLETMEIVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF
RSNGLTANESGRLIDFLKDVMDSMDKEEMEITQRLNKRSYLIRALTLNTM
TKDAERGKLKRRAIATPGMQIRGFVYFVEALARSICEKLEQSGLPVGGNE
KKAKLANVVRKMMTNSQDTELSFTITGDNTKWNENQNPRMFLAMITYITR
NQPEWFRNVLSIAPIMFSNKMARLGKGYMFESKSMKLRTQVPAEMLANID
LKYFNKSTREKIEKIRPLLIDGTASLSPGMMMGMFNMLSTVLGVSILNLG
QKKYTKTTYWWDGLQSSDDFALIVNAPNHEGIQAGVDRFYRTCKLVGINM
SKKKSYINRTGTFEFTSFFYRYGFVANFSMELPSFGVSGINESADMSVGV
TVIKNNMINNDLGPATAQMALQLFIKDYRYTYRCHRGDTQIQTRRAFELK
KLWEQTRSKAGLLVSDGGPNLYNIRNLHIPEVCLKWELMDEDYQGRLCNP
MNPFVSAVATTHSWIPKR

Ligand information

>7zpl Chain U (length=12)

aguaguaacaag

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Receptor-Ligand Complex Structure

Structure summary

• PDB: 7zpl Direct observation of backtracking by influenza A and B polymerases upon consecutive incorporation of the nucleoside analog T1106.
• Resolution: 3.12 Å
• Binding residue (original residue number in PDB): H32 G33 T34 M356 R365
• Binding residue (residue number reindexed from 1): H32 G33 T34 M329 R338

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019083 viral transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0005737 cytoplasm
• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:7zpl, PDBe:7zpl, PDBj:7zpl
• PDBsum: 7zpl
• PubMed: 36596301
• UniProt: M9TLW3