Structure of PDB 7z4d Chain E Binding Site BS01

Receptor Information
>7z4d Chain E (length=1166) Species: 1314 (Streptococcus pyogenes) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGAL
LFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRL
EESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADL
RLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPI
NASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPN
FKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAIL
LSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIF
FDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRK
QRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKN
LPNEKVLPKHSLLYEYFTVYNELTKQKKAIVDLLFLKEDYFKKIECFSLG
TYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHL
FDDKVMKQLKRRRYTGWGRLSRKLINIRDKQSGKTILDFLKSANRNFMQL
IHDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMAA
IVPQSFLKDDSIDNKSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKY
DENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVV
GTALIKKYPKLESEFVYGDYKVYDMIAKSEQEKYFFYSNIMNFFKTEITL
ANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQT
GGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKG
KSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYS
LFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDN
EQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPI
REQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSI
TGLYETRIDLSQLGGD
Ligand information
>7z4d Chain F (length=77) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
auaaagaugagacgcguuuuagagcuagaaauagcaaguuaaaauaaggc
uaguccguuaucaacuugaaaaaguga
...............<<<<<<..<<<<....>>>>....>>>>>>..<<.
....>>.......<<<<....>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
[Spin on] [Spin off] [Reset]
[High quality] [Low quality]
[White background] [Black background]
PDB7z4d R-loop formation and conformational activation mechanisms of Cas9.
Resolution3.1 Å
Binding residue
(original residue number in PDB)		K30 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 K76 N77 L101 S104 F105 V107 K111 R115 H116 I122 V126 H129 I135 H160 K163 F164 R165 G166 H167 Y325 H328 H329 L332 K336 R340 F351 F352 Y359 I363 K401 Q402 R403 T404 F405 N407 G408 F446 I448 Y450 G453 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 P475 H721 K735 Q739 K742 R753 K1097 E1099 V1100 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 W1126 K1130 Y1131 M1169 H1349 Q1350 S1351 Y1356 E1357
Binding residue
(residue number reindexed from 1)		K29 E56 T57 A58 T61 R62 K64 R65 R68 R69 R70 Y71 R73 K75 N76 L100 S103 F104 V106 K110 R114 H115 I121 V125 H128 I134 H159 K162 F163 R164 G165 H166 Y324 H327 H328 L331 K335 R339 F350 F351 Y358 I362 K400 Q401 R402 T403 F404 N406 G407 F445 I447 Y449 G452 L454 A455 R456 N458 S459 F461 W463 R466 T471 I472 P474 H656 K670 Q674 K677 R688 K895 E897 V898 G901 G902 F903 I908 P910 K911 R920 K921 K922 W924 K928 Y929 M967 H1147 Q1148 S1149 Y1154 E1155
Enzymatic activity
Enzyme Commision number 3.1.-.-
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:7z4d, PDBe:7z4d, PDBj:7z4d
PDBsum7z4d
PubMed36002571
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

[Back to BioLiP]