Structure of PDB 7wtw Chain E Binding Site BS01

Receptor Information

>7wtw Chain E (length=262) Species: 9606 (Homo sapiens)

ARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNR
LKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRL
IYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPD
PLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERH
PGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEE
RDKRLAAKQSSG

Ligand information

>7wtw Chain 2 (length=1586)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucguacuuggauaacugugguaa
uucuagagcuaauacaugccgacgggcgcugacccccuucgcggggggga
ugcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacg
ccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggua
gucgccgugccuaccauggugaccacgggugacggggaaucaggguucga
uuccggagagggagccugagaaacggcuaccacauccaaggaaggcagca
ggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaauaa
caauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaaa
uccuuuaacgaggauccauuggagggcaagucuggugccagcagccgcgg
uaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcuc
guaguuggaucucucucggccgaagcguuuacuuugaaaaaauuagagug
uucaaagcaggccgccuggauaccgcagcuaggaauaauggaauaggacc
gcgguucuauuuuguugguuuucggaacugaggccaugauuaagagggac
ggccgggggcauucguauugcgccgcuagaggugaaauucuuggaccggc
gcaagacggaccagagcgaaagcauuugccaagaauguuuucauuaauca
agaacgaaagucggagguucgaagacgauaccgucguaguuccgaccaua
aacgaugccgaccggcgaugcggcggcguuauucccaugacccgccgggc
agcuuccgggaaaccaaagucuuuggguuccggggggaguaugguugcaa
agcugaaacuuaaaggaauugacggaagggcaccaccaggaguggagccu
gcggcuuaauuugcacccggcccgacacggacaggauugacagauugaua
gcucuuucucgauuccgugggugguggugcauggccguuguuggcugguu
aauuccgaugaacgagacucuggcaugcuauucuuagagggacaaguggc
guucagccacccgagauugagcuaacaggucugugaugcccuguccgggg
cugcacgcgcgcuacacugacuggcucagcguguccuacgccggcaggcg
cggguaacccguugaaccccauucgugauggggaucggggauugcaauua
uuccccaugaacgaggaauucccaguaagugcgggucauaagcuugcguu
gauuaagucccugcccuuuguacacaccgcccgucgcuacuaccgauugg
augguuuagugaggcccucggaucggccccgccggggugcccuggcggag
cgcugagaagacggucgaacuugacuaucuagaggaaguaaaagucgagg
uuuccguaggugaaccugcggaaggaucauuaacgg

...<<<<<.[[((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<..<<....<<....<<..........>>...>>.>>......<<...
.....<<<.<....<<....<<<<<.........<<.....<<.<<<...
..>>>.>>......>>.........<<<<...<<<<<<....>>>>>>..
.>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<
<..>>>>>>>.>>.>>>...>>..>.>>>.<<<....<<<....<<<<<<
<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>..
..>>>.>>.<<.<<<..........>>>.>>.<.<<....>>.>...>>>
>>>.........<<<....<<<<...>>>>..>>>..>...>>.>.....
<<<<<<<<<.<<<....>>>..>>>>>>.>>>......<<..<.......
....>..>>.........<<<<<((......<<<<.....<<..))>>..
.....>>>>.>>>>>..>>>>>>.>>>.........<.<((.....<.<<
...<<<.<<...<<.<<<..>>>>>...<<<<<<.<.......<<...<.
......>.<<<<.>>>>...>>......>.>>>..>>>........<.<<
.<<<<<<<...............>>>>>>>.>>.>....>>....<<<<<
<..<...<<<<..<<<.<<<<<<<<...<<<......>>>......>>>>
>>>>.>>>.......<<....>>...>>>>..>..>>>.>>>...>>>..
.>>.>....<<<<<<<...<...<<<<....>>>>...>>>>>>>>....
......<<<.<<.<<<..<.<<<<<<.<<<........>>>>>>>>>.>.
.>>>...<<..))>>...>>.....>>>.>.>.<<<......<<<<....
>>>>....>>>..)))).]]....<<<<<<<<..<<..<<<<<.<<<<.<
<<<<<..........<<<<<.....<<<<<<.......<<.<<<......
..>>>.>>.....>>>>>>......<<<.<<.<<<<<<.<<<...<<<..
....>>>...>>>....<<<...<...<<<....>>>..>.....<<<<<
.....>>>>>........>>>...>>.>>>>..>>..>>>....>>>>>.
..>>.>>>>.>>>.>...<<.<<<........<.<<<.<<<<....>>>>
.>>>....>.........>>>.>>......<.....<<<<<.........
.>>>>>.....>.....>>>>>.....<<<<<<<<<.....>>>>>>>>>
......>>...>>>>>>>>......<<<...<<....<<<<.<<....<<
<<<<<<.<<<..<<<<.<<...<<<<<<<<<<<<<<<..>>>>>>>>>.>
.>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.>>>>.....>>.>>
><<<<<<<<<....>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7wtw The nucleoplasmic phase of pre-40S formation prior to nuclear export.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): A2 R3 G4 P5 K7 H8 K10 V12 M19 D21 K22 T24 F27 A28 P29 R30 T33 G34 K37 L38 R49 K62 M66 K106 R108 V131 G132 K134 V140 A144 T146 R148 K155 G186 A187 E199 H201 P202 G203 A219 R221
• Binding residue (residue number reindexed from 1): A1 R2 G3 P4 K6 H7 K9 V11 M18 D20 K21 T23 F26 A27 P28 R29 T32 G33 K36 L37 R48 K61 M65 K105 R107 V130 G131 K133 V139 A143 T145 R147 K154 G185 A186 E198 H200 P201 G202 A218 R220

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0008284 positive regulation of cell population proliferation
• GO:0042274 ribosomal small subunit biogenesis
• GO:0045727 positive regulation of translation

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0005925 focal adhesion
• GO:0015935 small ribosomal subunit
• GO:0016020 membrane
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0032040 small-subunit processome
• GO:0036464 cytoplasmic ribonucleoprotein granule
• GO:0045202 synapse
• GO:0070062 extracellular exosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7wtw, PDBe:7wtw, PDBj:7wtw
• PDBsum: 7wtw
• PubMed: 36321656
• UniProt: P62701|RS4X_HUMAN Small ribosomal subunit protein eS4, X isoform (Gene Name=RPS4X)