Structure of PDB 7uvz Chain E Binding Site BS01

Receptor Information

>7uvz Chain E (length=200) Species: 480119 (Acinetobacter baumannii AB0057)

MNLKTVSGSAVELSEVAFGREFNEALVHQVVTAYLAGGRQGTRAHKSRAD
VSGGGKKPFRQKGTGRARAGSIRSPIWVGGGKTFAARPQDWSQKVNRKMY
RGAMQCILAELVRQDRLVLVEEFAVAAPKTKELLAKLNDLNAARALIVTD
AVDENLYLAARNLPHVDVVDATAIDPVSLIAFDKVVMSVAAAKKIEVELG

Ligand information

>7uvz Chain A (length=2730)

gucaaguaauuaagugcaugugguggaugccuuggcagucagaggcgaug
aaagacgugauagccugcgaaaagcuccggggaggcggcaaauauccuuu
gauccggagaugucugaaugggggaacccaccuacuuuaagguagguauu
gcaacaugaauacauaguguugcaaggcgaacgaggggaagugaaacauc
ucaguacccuuaggaaaagaaaucaauugagauucccucaguagcggcga
gcgaacggggaucagcccauuaaguuauguguguuuuaguggaacgcucu
gggaagugcgaacguagagggugauauucccguacacgaaagggcacaca
uaaugaugacgaguagggcgaggcacgugaaaccuugucugaauaugggg
ggaccauccuccaaggcuaaauacuccugacugaccgauagugaaccagu
accgugagggaaaggcgaaaagaaccccugugaggggagugaaauagauc
cugaaaccgcaugcauacaagcagugggagcaccuucguggugugacugc
guaccuuuuguauaaugggucagcgacuuauauucaguagcgagguuaac
cguauaggggagccguagagaaaucgagucuuaauagggcguuuaguugc
uggguauagacccgaaaccaggcgaucuauccaugagcagguugaagguu
ggguaacacuaacuggaggaccgaacccacugucguugaaaagccagggg
augacuuguggauaggggugaaaggcuaaucaagccuggugauagcuggu
ucuccccgaaagcuauuuagguagcgccucggacgaauaccauagggggu
agagcacuguuucggcaccgaugcaaacuccgaauaccuaugaguacuau
ccgggagacagacugcgggugcuaacguccguagucaagaggaaaacaau
ccagaccgccagcuaaggccccaaaaucauaguuaagugggaaacgaugu
gggaaggcauagacagcuauagucgagucggccugcgcggaagauguaac
ggggcuaaaacuaugugccgaagcugcggauguauacuuuguauacgugg
uaggggagcguucuguaagccgaugaagguguguugagaagcaugcugga
gguaucagaagugcgaaugcugacgugaguaacgacaaaacgggugaaaa
acccguucgccgaaagaccaaggguuccaguccaacguuaaucggggcug
ggugagucgaccccuaaggcgaggccgaaaggcguagucgaugggaaaau
gguuaauauuccauuacuucuguguaaugcgaugagaggacggagaaggc
uaaaucagccuggcguugguuguccaggugaaaggauguaggcauguauc
uuaggcaaauccgggguacucuaugcugagaucugauagcaagcuguacu
ugugcagcgaagugguugaugccaugcuuccaggaaaagucucuaagcuu
caguuacacaggaaucguacccgaaaccgacacagguggucaggucgagu
agaccaaggcgcuugagagaacucugcugaaggaacuaggcaaaauggua
ccguaacuucgggagaagguacgcuguuguuggugauggaacucgcuucc
ugagcugacgacagccgcagaaaccaggccgcugcaacuguuuauuaaaa
acauagcacucugcaaacacgaaaguggacguauagggugugaugccugc
ccggugcuggaagguuaauugaugggguuagcguaagcgaagcucuugau
cgaagccccaguaaacggcggccguaacuauaacgguccuaagguagcga
aauuccuugucggguaaguuccgaccugcacgaauggcauaaugauggcg
gcgcugucuccagcagaggcucagugaaaucgaaaucgcugugaagaugc
aguguacccgcggcuagacggaaagaccccgugaaccuuuacugcagcuu
gacacugaacuuugauugagguucuaacucugucccgugauccgggacga
ggaccgugucugguggguaguuugacuggggcggucuccuccuaaagagu
aacggaggaguacgaaggugcgcucagcguggucggaaaucacgcguaga
guauaaaggcaaaagcgcgcuuaacugcgagacccacaagucgagcaggu
acgaaaguaggucuuagugauccggugguucuguauggaagggccaucgc
ucaacggauaaaagguacucuggggauaacaggcugauaccgcccaagag
uucauaucgacggcgguguuuggcaccucgaugucggcucaucucauccu
ggggcugaagcaggucccaaggguauggcuguucgccauuuaaagaggua
cgcgagcuggguuuagaacgucgugagacaguucggucccuaucuaccgu
gggcgcuggaaauuugagaggaucugcuccuaguacgagaggaccagagu
ggacgaaccucugguguaccgguugugacgccagucgcaucgccggguag
cuauguucggaagggauaaccgcugaaagcaucuaagcgggaagccuacc
ucaagauaagauuucccuaggacuuuauguccucuaaagagccguucgag
acuaggacguugauagguuggauguggaagcauagugauaugugaagcug
accaauacuaauugcucgugaggcuugacu

<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<....
..<<<<<<.[..(((.>>>.....<<<<<<<..<<.)))...]..>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>
......>>>>>.>.........<<....>>...<<<<<.....<<.....
>>....>>>>>...<<<......<<<<<<<<<<<<<<.<<<...<<<.<.
.....>.>>>((......<<<.....))>>>...>>>...>>>>>>>>>>
>>>>...........<<<<<<<<.........>>>>>>>>......<<<<
<..<<<<>>>>>..>>>......]].>>>>>>>.]]..............
.<<....>>....]].........<<<<.....>>>>.............
.....>>>>>>>>>>>.......<<<<..<<<<<.....>>>>>..>>>>
...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<
<.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>
>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<<...<<<<
<<......>>>>>>.>...>>>...<<<.<<<.<........>.>>>>>>
....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>.>>.
.<<<<<<....<<<<<....>>>>>.<<<<<<<....<<<<<<<<<....
.<<<...<<<.<<<<..>>>>.>>>..>>.>.....>>>>>>.>>>...>
>>>>>.>...<<<<<<<<<<......>>>>>>>>>>....<<<......>
>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<<
<<<..<<........<<<..>>>....>>..>>>>>.>>...........
)))......>>>>>>.>>>...>>>>>>><<<<<<<.....>>>>>>>..
..>>>>>>..<<<<<...<<<......<<<<<<<<....>>>>>>>>...
>>>..>>>>>........>>>>>><......<<.....<<<<<<......
.>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>
>....((<<<.<<<...<<<...<<<....>>>...>>>...>>>..<<<
<<........>>>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<
..<<<<<<<<<<<...((((...>>>>>>....<<<<...<<<<.<<<<<
<..<<.....>>.>>>>>>....>>>>...>>>>........<<<<<...
...>>>>>....>>>>>...>>>...>>>>.........>>>>...>>..
..>>>>>>>>>>>.>>>..>>.....<<......>>>>>>>.<<<<....
.>>>>..>>>>..<<<<....<<<<<<<<.<<.........<.....<<<
<<.....<..>......>>>>><<<<<<<<<<<<...<<<<.....>>>>
...>>>>>>>>>>>>............<<<<<<<<<..<<<.........
>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<
<..<<<<<<<..<<<<.......<<<<<..<<....>>...>>>>>....
...>>>>>>>>>..>>>>><<<<<<.......>>>>>>........<...
........<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>
>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>
>>>>..<<<<<<<.<<<<.[[......<<<<.[[<<<<..<<<<..<<<.
<<<<<.<<<<<<<<..>>>>>>>>...<<<.<<<<<<.....>>>>>>>>
>....>>>>>.>>>...>>>>..<<<<.....>>>><<<<<<........
...>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....
<<.[.(((>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<
<<....>>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>.
............>>>>..]]>>>>.......<<<<...<<<<<<...<<.
......>>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<
<<<<<<......>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>.
.>...>>>.>>....<<<<<<<....>>>.>>>>....]]..>>>>>>>>
>>>....<<<<<<<...<<<<..<<<<<<.................>>>>
>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>
>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>>
>>.......>>>>>>>..<<<<<.....>>>>><....<<<<<<<<<...
....>>>>>....<<<<<<<<..<<....<<<<<....>>>>>...>>..
.>>>>>.>>>...>>>>..>..>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7uvz Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.
• Resolution: 2.21 Å
• Binding residue (original residue number in PDB): N23 H28 Q29 Y34 L35 R39 Q40 T42 R43 A44 H45 K46 R48 A49 V51 S52 G53 K56 K57 Q61 K62 G63 G65 R66 A67 R68 G70 R73 P75 I76 W77 V78 G79 K82 T83 F84 W91 Q93 K94 N96 R97 K98 M99 Y100 P128 K129 T130 K131 L158 R161 N162 P164
• Binding residue (residue number reindexed from 1): N23 H28 Q29 Y34 L35 R39 Q40 T42 R43 A44 H45 K46 R48 A49 V51 S52 G53 K56 K57 Q61 K62 G63 G65 R66 A67 R68 G70 R73 P75 I76 W77 V78 G79 K82 T83 F84 W91 Q93 K94 N96 R97 K98 M99 Y100 P128 K129 T130 K131 L158 R161 N162 P164

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7uvz, PDBe:7uvz, PDBj:7uvz
• PDBsum: 7uvz
• PubMed: 37192172
• UniProt: B7IA38|RL4_ACIB5 Large ribosomal subunit protein uL4 (Gene Name=rplD)