Structure of PDB 7uvw Chain E Binding Site BS01

Receptor Information

>7uvw Chain E (length=200) Species: 480119 (Acinetobacter baumannii AB0057)

MNLKTVSGSAVELSEVAFGREFNEALVHQVVTAYLAGGRQGTRAHKSRAD
VSGGGKKPFRQKGTGRARAGSIRSPIWVGGGKTFAARPQDWSQKVNRKMY
RGAMQCILAELVRQDRLVLVEEFAVAAPKTKELLAKLNDLNAARALIVTD
AVDENLYLAARNLPHVDVVDATAIDPVSLIAFDKVVMSVAAAKKIEVELG

Ligand information

>7uvw Chain A (length=2719)

gucaaguaauuaagugcaugugguggaugccuuggcagucagaggcgaug
aaagacgugauagccugcgaaaagcuccggggaggcggcaaauauccuuu
gauccggagaugucugaaugggggaacccaccuacuuuaagguagguauu
gcaacaugaauacauaguguugcaaggcgaacgaggggaagugaaacauc
ucaguacccuuaggaaaagaaaucaauugagauucccucaguagcggcga
gcgaacggggaucagcccauuaaguuauguguguuuuaguggaacgcucu
gggaagugcgaacguagagggugauauucccguacacgaaagggcacaca
uaaugaugacgaguagggcgaggcacgugaaaccuugucugaauaugggg
ggaccauccuccaaggcuaaauacuccugacugaccgauagugaaccagu
accgugagggaaaggcgaaaagaaccccugugaggggagugaaauagauc
cugaaaccgcaugcauacaagcagugggagcaccuucguggugugacugc
guaccuuuuguauaaugggucagcgacuuauauucaguagcgagguuaac
cguauaggggagccguagagaaaucgagucuuaauagggcguuuaguugc
uggguauagacccgaaaccaggcgaucuauccaugagcagguugaagguu
ggguaacacuaacuggaggaccgaacccacugucguugaaaagccagggg
augacuuguggauaggggugaaaggcuaaucaagccuggugauagcuggu
ucuccccgaaagcuauuuagguagcgccucggacgaauaccauagggggu
agagcacuguuucggcaccgaugcaaacuccgaauaccuaugaguacuau
ccgggagacagacugcgggugcuaacguccguagucaagaggaaaacaau
ccagaccgccagcuaaggccccaaaaucauaguuaagugggaaacgaugu
gggaaggcauagacagcuauagucgagucggccugcgcggaagauguaac
ggggcuaaaacuaugugccgaagcugcggauguauacuuuguauacgugg
uaggggagcguucuguaagccgaugaagguguguugagaagcaugcugga
gguaucagaagugcgaaugcugacgugaguaacgacaaaacgggugaaaa
acccguucgccgaaagaccaaggguuccaguccaacguuaaucggggcug
ggugagucgaccccuaaggcgaggccgaaaggcguagucgaugggaaaau
gguuaauauuccauuacuucuguguaaugcgaugagaggacggagaaggc
uaaaucagccuggcguugguuguccaggugaaaggauguaggcauguauc
uuaggcaaauccgggguacucuaugcugagaucugauagcaagcuguacu
ugugcagcgaagugguugaugccaugcuuccaggaaaagucucuaagcuu
caguuacacaggaaucguacccgaaaccgacacagguggucaggucgagu
agaccaaggcgcuugagagaacucugcugaaggaacuaggcaaaauggua
ccguaacuucgggagaagguacgcuguuguuggugauggaacucgcuucc
ugagcugacgacagccgcagaaaccaggccgcugcaacuguuuauuaaaa
acauagcacucugcaaacacgaaaguggacguauagggugugaugccugc
ccggugcuggaagguuaauugaugggguuagcguaagcgaagcucuugau
cgaagccccaguaaacggcggccgguccuaagguagcgaaauuccuuguc
ggguaaguuccgaccugcacgaauggcauaaugauggcggcgcugucucc
agcagaggcucagugaaaucgaaaucgcugugaagaugcaguguacccgc
ggcuagacggaaagaccccgugaaccuuuacugcagcuugacacugaacu
uugauugagguucuaacucugucccgugauccgggacgaggaccgugucu
gguggguaguuugacuggggcggucuccuccuaaagaguaacggaggagu
acgaaggugcgcucagcguggucggaaaucacgcguagaguauaaaggca
aaagcgcgcuuaacugcgagacccacaagucgagcagguacgaaaguagg
ucuuagugauccggugguucuguauggaagggccaucgcucaacggauaa
aagguacucuggggauaacaggcugauaccgcccaagaguucauaucgac
ggcgguguuuggcaccucgaugucggcucaucucauccuggggcugaagc
aggucccaaggguauggcuguucgccauuuaaagagguacgcgagcuggg
uuuagaacgucgugagacaguucggucccuaucuaccgugggcgcuggaa
auuugagaggaucugcuccuaguacgagaggaccagaguggacgaaccuc
ugguguaccgguugugacgccagucgcaucgccggguagcuauguucgga
agggauaaccgcugaaagcaucuaagcgggaagccuaccucaagauaaga
uuucccuaggacuuuauguccucuaaagagccguucgagacuaggacguu
gauagguuggauguggaagcauagugauaugugaagcugaccaauacuaa
uugcucgugaggcuugacu

<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<....
..<<<<<<....(((.>>>.....<<<<<<<..<<.)))......>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>
......>>>>>.>.........<<<..>>>...<<<<<.....<<.....
>>....>>>>>...<<<......<<<<<<<<<<<<<..<<<...<<<.<.
.....>.>>>((......<<<.....))>>>...>>>....>>>>>>>>>
>>>>...........<<<<<<<<.........>>>>>>>>......<<<<
<..<<<<>>>>>..>>>......]].>>>>>>>.]]..............
.<<....>>....]].........<<<<.....>>>>.............
.....>>>>>>>>>>>.......<<<<..<<<<<.....>>>>>..>>>>
...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<
<.....>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>
>>>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<<...<<<<
<<......>>>>>>.>...>>>...<<<.<<<.<........>.>>>>>>
....>>>>>>>>>...<<<.....>>>......>>>>>>...>..>.>>.
.<<<<<<....<<<<<....>>>>>.<<<<<<<....<<<<<<<<<....
.<<<...<<<.<<<<..>>>>.>>>..>>.>.....>>>>>>.>>>...>
>>>>>.>...<<<<<<<<<<......>>>>>>>>>>....<<<......>
>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<<
<<<..<<........<<<..>>>....>>..>>>>>.>>...........
)))......>>>>>>.>>>...>>>>>>><<<<<<<.....>>>>>>>..
..>>>>>><.<<<<<...<<<......<<<<<<<<....>>>>>>>>...
>>>..>>>>>..>.....>>>>>><......<<.....<<<<<<......
.>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>
>....((<<<.<<<...<<<...<<<....>>>...>>>...>>>..<<<
<<........>>>>>))<<<<<<<<<<<<<...<<<<....<<<<..<<<
..<<<<<<<<<<<...((((...>>>>>>....<<<<...<<<<.<<<<<
<..<<.....>>.>>>>>>....>>>>...>>>>........<<<<<...
...>>>>>....>>>>>...>>>...>>>>.........>>>>...>>..
..>>>>>>>>>>>.>>>..>>.....<<......>>>>>>>.<<<<....
.>>>>..>>>>..<<<<....<<<<<<<<.<<.........<.....<<<
<<.....<..>......>>>>><<<<<<<<<<<<...<<<<.....>>>>
...>>>>>>>>>>>>............<<<<<<<<<..<<<.........
>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<
<..<<<<<<<..<<<<.......<<<<<..<<....>>...>>>>>....
...>>>>>>>>>..>>>>><<<..>>>........<...........<<<
<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>
>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<
<<.<<<<.[[......<<<<.[[<<<<..<<<<..<<<.<<<<<.<<<<<
<<<..>>>>>>>>...<<<.<<<<<<.....>>>>>>>>>....>>>>>.
>>>...>>>>..<<<<.....>>>><<<<<<...........>>>>>>..
<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>.
...>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>
>)))]......<<<<<<<.((..>>>>..))>>>>>>>............
.>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>...
>>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.....
.>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>
....<<<<<<<....>>>.>>>>....]]..>>>>>>>>>>>....<<<<
<<<...<<<<..<<<<<<.................>>>>>>.<<<<<...
<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...>>>>.>>>>>..
.<<<....<<<<<...........>>>>>....>>>.>>>>.......>>
>>>>>..<<<<<.....>>>>><....<<<<<<<<<.......>>>>>..
..<<<<<<<<..<<....<<<<<....>>>>>...>>...>>>>>.>>>.
..>>>>..>..>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7uvw Streptothricin F is a bactericidal antibiotic effective against highly drug-resistant gram-negative bacteria that interacts with the 30S subunit of the 70S ribosome.
• Resolution: 2.37 Å
• Binding residue (original residue number in PDB): N23 H28 Q29 Y34 L35 G38 R39 Q40 T42 R43 H45 K46 R48 A49 V51 G53 K56 Q61 K62 G63 G65 R66 A67 R68 G70 P75 I76 V78 G79 K82 T83 F84 K94 N96 R97 K98 M99 Y100 R101 P128 K131 L158 R161 N162 H165
• Binding residue (residue number reindexed from 1): N23 H28 Q29 Y34 L35 G38 R39 Q40 T42 R43 H45 K46 R48 A49 V51 G53 K56 Q61 K62 G63 G65 R66 A67 R68 G70 P75 I76 V78 G79 K82 T83 F84 K94 N96 R97 K98 M99 Y100 R101 P128 K131 L158 R161 N162 H165

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7uvw, PDBe:7uvw, PDBj:7uvw
• PDBsum: 7uvw
• PubMed: 37192172
• UniProt: B7IA38|RL4_ACIB5 Large ribosomal subunit protein uL4 (Gene Name=rplD)