Structure of PDB 7syk Chain E Binding Site BS01

Receptor Information

>7syk Chain E (length=228) Species: 9986 (Oryctolagus cuniculus)

MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIII
LATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIA
QAESLRYKLLGGLAVRRACYGVLRFIMESGAKGCEVVVSGKLRGQRAKSM
KFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPSGKIGP
KKPLPDHVSIVEPKDEILPTTPISEQKG

Ligand information

>7syk Chain 2 (length=1697)

uaccugguugauccugccaguagcauaugcuugucucaaagauuaagcca
ugcaugucuaaguacgcacggccgguacagugaaacugcgaauggcucau
uaaaucaguuaugguuccuuuggucgcucgacuuggauaacugugguaau
ucuagagcuaauacaugccgacgggcgcugacccccuucgcgggggggau
gcgugcauuuaucaguggugacucuagauaaccucgggccgaucgcacgc
ccggcggcgacgacccauucgaacgucugcccuaucaacuuucgauggua
gucgccgugccuaccauggugaccacgggugacggggaaucaggguucga
uuccggagagggagccugagaaacggcuaccacauccaaggaaggcagca
ggcgcgcaaauuacccacucccgacccggggagguagugacgaaaaauaa
caauacaggacucuuucgaggcccuguaauuggaaugaguccacuuuaaa
uccuuuaacgaggauccauuggagggcaagucuggugccagcagccgcgg
uaauuccagcuccaauagcguauauuaaaguugcugcaguuaaaaagcuc
guaguuggaucuugggagcggccguccccugcucggcgccggcccgaagc
guuuacuuugaaaaaauuagaguguucaaagcaggccgccuggauaccgc
agcuaggaauaauggaauaggaccgcgguucuauuuuguugguuuucgga
acugaggccaugauuaagagggacggccgggggcauucguauugcgccgc
uagaggugaaauucuuggaccggcgcaagacggaccagagcgaaagcauu
ugccaagaauguuuucauuaaucaagaacgaaagucggagguucgaagac
gaucagauaccgucguaguuccgaccauaaacgaugccgaccggcgaugc
ggcggcguuauucccaugacccgccgggcagcuuccgggaaaccaaaguc
uuuggguuccggggggaguaugguugcaaagcugaaacuuaaaggaauuu
ggcgaagggcaccaccaggaguggagccugcggcuuaauuugacucaaca
cgggaaaccucacccggcccggacacggacaggauugacagauugauagc
ucuuucucgauuccgugggugguggugcauggccguucuuaguuggugga
gcgauuugucugguuaauuccgauaacgaacgagacucuggcaugcuaac
uaguuacgcgaccccggucggcguaacuucuuagagggacaaguggcguu
cagccacccgagauugagcaauaacaggucugugaugcccuuagaugucc
ggggcugcacgcgcgcuacacugacuggcucagcgugugccuacccuacg
ccggcaggcgcggguaacccguugaaccccauucgugauggggaucgggg
auugcaauuauuccccaugaacgaggaauucccaguaagugcgggucaua
agcuugcguugauuaagucccugcccuuuguacacaccgcccgucgcuac
uaccgauuggaugguuuagugaggcccucggaucggccccgccggggugc
ccuggcggagcgcugagaagacggucgaacuugacuaucuagaggaagua
aaagucguaacaagguuuccguaggugaaccugcggaaggaucauua

...<<<<<.[[((((>>>>><<<.<<<<<<...<.<<..<......<<<.
<<<...<....<<....<<..........>>...>>.>.......<<...
.....<<<.<....<<....<<<<.........<<.....<<.<<.....
..>>.>>......>>.........<<<<...<<<<<<....>>>>>>...
>>>><<..<<<<<...<......>..>>>>>......>>...<<<<.<<<
...>>>>>>>..>.>>>...>>..>.>>>.<<<....<<<....<<<<<<
<.........>>>>>>>>>>......>>>...<<<.<<<<....>>>>..
..>>>.>>.<<.<<<..........>>>.>>.<.<<<..>>>.>...>>>
>>>.........<<<....<<<.....>>>..>>>..>...>>.>.....
<<<<<<<<<.<........>..>>>>>>.>>>......<<..<.......
....>..>>.........<<<<<((....<.<<<<.....<<..))>>..
.....>>>>>>>>>>..>>>>>>.>>>.........<.<((.....<.<<
...<<<.<<.....<<.<<<..>>>.>>..<<<<<..<<..>>.>>>.>>
..<<<<<<.<.......<<...<.......>.<<<<.>>>>...>>....
..>.>>>..>>>........<.<<.<<<<<<<...............>>>
>>>>.>>.>....>>....<<<<<<......<<<<..<<..<<<<<<<<.
..<<<......>>>......>>>>>>>>..>>.......<<....>>...
>>>>.....>>>.>>>...>>>...>>.>....<<<<<<<...<...<<<
<.<.....>.>>>>...>>>>>>>>..........<<<.<<.<<<..<.<
<<<<<.<<<........>>>>>>>>>.>..>>>...<<..))>>...>>.
....>>>.>.>.<<<......<<<......>>>....>>>..)))).]].
<...<<<<<<<..<<..<<<<<..<<<.<<<<<<......<<........
>>..........<<<<<......<<<<<<.......<<.<<<........
>>>.>>.....>>>>>>.<.<<.<<<.....<<<.......<<<.<<<<<
....>>>...<<<......>>>...>>.>>>....<<<...<...<<<<.
.<<<<<<<<<<<<..>>>>.>>>>>>>>..>>>>..>.....<<<<<...
..>>>>>........>>>........>>>.......>>>>>>......>>
>>>...>>.>>>>.>>>.....<<.<<<........<.......<<<.<<
<<....>>>>.>>>....>.........>>>.>>......<...<.<<<<
<..........>>>>>>....>.....>>>>>.....<<<<<<<<.....
..>>>>>>>>......>>...>>>>>>>..>.......<..<<.<..<<<
<.<<....<<<<<<<<.<<<..<<<<.<<...<<<<<..<<<<<<<<..>
>>>>>>>....>>>>>...>>.>>>>..>>>.>>>>>>>>...>>.>>>>
...>.>>...>.....<<<<<<<<<....>>>>>>>>>.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7syk Molecular architecture of 40S translation initiation complexes on the hepatitis C virus IRES.
• Resolution: 4.2 Å
• Binding residue (original residue number in PDB): Q4 S6 K8 R9 R27 S139 K141 R143 R146 K151 L156 M157 I158 H159 S160 G161 D162 P163 H174 L176 Q179 V181 K185 P205 D206
• Binding residue (residue number reindexed from 1): Q4 S6 K8 R9 R27 S139 K141 R143 R146 K151 L156 M157 I158 H159 S160 G161 D162 P163 H174 L176 Q179 V181 K185 P205 D206

Enzymatic activity

• Enzyme Commision number: 4.2.99.18: DNA-(apurinic or apyrimidinic site) lyase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity
• GO:0016829 lyase activity
• GO:0140078 class I DNA-(apurinic or apyrimidinic site) endonuclease activity

Biological Process

• GO:0006281 DNA repair
• GO:0006412 translation
• GO:0006417 regulation of translation
• GO:0006915 apoptotic process
• GO:0031334 positive regulation of protein-containing complex assembly
• GO:0051092 positive regulation of NF-kappaB transcription factor activity
• GO:0051301 cell division
• GO:2001235 positive regulation of apoptotic signaling pathway

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0005819 spindle
• GO:0005840 ribosome
• GO:0005856 cytoskeleton
• GO:0015935 small ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0045202 synapse
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7syk, PDBe:7syk, PDBj:7syk
• PDBsum: 7syk
• PubMed: 35822879
• UniProt: G1TNM3|RS3_RABIT Small ribosomal subunit protein uS3 (Gene Name=RPS3)