Structure of PDB 5h9f Chain E Binding Site BS01

Receptor Information
>5h9f Chain E (length=354) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSLKRAMRKS
GYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKSVD
EAEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIR
VNLQQGVDIALSGRMATSGMMTELGKVDGAMSIAHAITTHQVDSDIDWFT
AVDDLQEQGSAHLGTQEFSSGVFYRYANINLAQLQENLGGASREQALEIA
THVVHMLATEVPGAKQRTYAAFNPADMVMVNFSDMPLSMANAFEKAVKAK
DGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSLTAQVKQMPTLEQLKSWV
RNNG
Ligand information
>5h9f Chain L (length=61) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
auaaaccgacgguauuguucagauccuggcuugccaacaggaguuccccg
cgccagcgggg
..............................................<<<<
<....>>>>>.
Receptor-Ligand Complex Structure
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PDB5h9f Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli.
Resolution2.45 Å
Binding residue
(original residue number in PDB)		R20 D21 D22 K27 S40 Q42 S43 K45 R49 M166 W199 F200 T201 V203 A265 K266
Binding residue
(residue number reindexed from 1)		R19 D20 D21 K26 S39 Q41 S42 K44 R48 M165 W198 F199 T200 V202 A264 K265
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0071667 DNA/RNA hybrid binding
Biological Process
GO:0051607 defense response to virus
GO:0099048 CRISPR-cas system
Cellular Component
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5h9f, PDBe:5h9f, PDBj:5h9f
PDBsum5h9f
PubMed26863189
UniProtQ46899|CASC_ECOLI CRISPR system Cascade subunit CasC (Gene Name=casC)

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