Structure of PDB 5h1s Chain E Binding Site BS01

Receptor Information
>5h1s Chain E (length=247) Species: 3562 (Spinacia oleracea) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
NPRNNLISGQRRCGKGRNARGIITARHRGGGHKRLYRKIDFRRNEKDIYG
KIVTIEYDPNRNAYICLIHYGDGEKRYILHPRGAIIGDTIVSGTEVPIKM
GNALPLTDMPLGTAIHNIEITLGRGGQLARAAGAVAKLIAKEGKSATLKL
PSGEVRLISKNCSATVGQVGNVGVNQKRLGRAGSKRWLGKRPVVRGVVMN
PVDHPHGGGEGRAPIGRKSPTTPWGYPALGRRSRKRNKYSDNFIIRR
Ligand information
>5h1s Chain A (length=2799) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uucaaacgaggaaaggcuuacgguggauaccuaggcacccagagacgagg
aagggcguauuaaucgacgaaaugcuucggggaguugaaaauaagcagag
auccggagauucccgaauaggucaaccuuucgaacuucugcugaauccau
gggcaggcaagagacaaccuggcgaacugaaacaucuuaguagccagagg
aaaagaaagcaaaagcgauucccguaguagcggcgagcgaaaugggagca
gccuaaaccgugaaaacgggguugugggagagcaauacaagcgucgugcu
gcuaggcgaaucaguggagugcggaacccuagauggugaaaguccaguag
ccgaaagcaucacuagcuuaugcucugacccgaguagcauggggcacgug
gaaucccgugugaaucagcaaggaccaccuugcaaggcuaaauacuccug
ggugaccgauagcgaaguaguaccgugagggaagggugaaaagaaccccc
aucggggagugaaauagaacaugaaaccguaagcucucaagcagugggag
ggggaacccugaccgcgugccuguugaagaaugagccggcgacucauagg
caguggcuugguuaagggaacccaccggagccguagcgaaagcgagucuu
cauagggcaauugucacugcuuauggacccgaaccugggugaucuaucca
ugaccaggaugaagcuugggugaaacuaaguggagguccgaaccgacuga
uguugaagaaucagcggaugaguugugguuaggggugaaaugccacucga
acccagagcuagcugguucuccccgaaaugcguugaggcgcagcaguuga
cuggacaucuagggguaaagcacuguuucggugcgggccgcgagagcggu
accaaaucgaggcaaacucugaauacuagauaugaccuccaaauaacagg
ggucaaggucggccagugagacgaugggggauaagcuucaucgucgagag
ggaaacagcccggaucaccagcuaaggccccuaaaugaccgcucagugau
aaaggagguaggggugcagagacagccaggagguuugccuagaagcagcc
acccuugaaagagugcguaauagcucacugaucgagcgcucuugcgccga
agaugaacggggcuaagcggucugccgaagcugugggauguaaaaaaaca
ucgguaggggagcguuccguguuagggagaaacgcgugcgugagccgcgu
uggacgaagcggaagcgagaaugucggcuugaguaacgcaaacauuggug
agaauccaaugccccgaaaaccuaaggguuccuccgcaagguucguccac
ggagggugagucagggccuaagaucaggccgaaaggcguagucgauggac
aacaggugaauauuccuguacuaccccuuguuggucccgagggacggagg
aggcuagguuagccgaaagaugguuaucgguucaaggacgcaaggugacc
cugggguaagaagggguagagaaaaugccucgagccaauguucgaguacc
aggcgcuacggcgcugaaguaaccgaugccauacucccaggaaaagcucg
aacgaccuucaacaaaaggguaccuguacccgaaaccgacacagguaggu
agguagagaauaccuaggggcgcgagacaacucucucuaaggaacucggc
aaaauagccccguaacuucgggagaaggggugcccccucacaaagggggu
cgaagugaccaggcccgggcgacuguuuaccaaaaacacaggucuccgca
aagucguaagaccauguaugggggcugacgccugcccagugccggaaggu
caaggaaguuggugaccugaugacaggggagccggcgaccgaagccccgg
ugaacggcggccguaacuauaacgguccuaagguagcgaaauuccuuguc
ggguaaguuccgacccgcacgaaaggcguaacgaucugggcacugucucg
gagagaggcucggugaaauagacaugucugugaagaugcggacuaccugc
accuggacagaaagacccuaugaagcuuuacuguucccugggauuggcuu
ugggcuuuuccugcgcagcuuagguggaaggcgaagaaggcccccuuccg
ggggggcccgagccaucagugagauaccacucuggaagagcuagaauucu
aaccuugugucaggaccuacgggccaagggacauucucagguagacaguu
ucuauggggcguaggccucccaaaagguaacggaggcgugcaaagguuuc
cucgggccggacggagauuggcccucgagugcaaaggcagaagggagcuu
gacugcaagacccacccgucgagcagggacgaaagucggccuuagugauc
cgacggugccgaguggaagggccgucgcucaacggauaaaaguuacucua
gggauaacaggcugaucuuccccaagaguucacaucgacgggaagguuug
gcaccucgaugucggcucuucgccaccuggggcuguaguauguuccaagg
guugggcuguucgcccauuaaagcgguacgugagcuggguucagaacguc
gugagacaguucgguccauauccggugugggcguuagagcauugagagga
ccuuucccuaguacgagaggaccgggaaggacgcaccucugguguaccag
uuaucgugcccacgguaaacgcuggguagccaagugcggagcggauaacu
gcugaaagcaucuaaguaguaagcccaccccaagaugagugcucuccua
..............<<<<<<<<<<.....<<..<<<<<<<<<<<......
..<<<<<<.....((.>>>.....<<<<<<.<.<<.))......>>....
.>>>>>>>...>>>....<<<....>>>........<<<<..........
...>>>>..........<<<<<<.....<......>......>>>>>.>.
..<.....<<....>>..<<<<<<.....<<.....>>....>>>>>>..
<<<....<<<<....>>>>.......<<.<<<<......<<<...<<<.<
<<<.<<<...<<.<<.....>>.>>((......<<<.....))>>>...>
>>...>>>>.>>>..>>>...>>>>...>>......<<<<<<<<......
...>>>>>>>>......<<<<<..((((>>>>>..>>>....>.>>.>>>
>>>>.>>...............<<....>>....>>.........<<<<.
...>>>>..................>>>>>>>>>>........<<<<..<
<<<...>>>>..>>>>...<.<........>.>.<<<<<<....<<<<<<
<<<<<<...<<<...<<.......>>...>>>...<<....>>..<<<<.
....>>>>.....>>>>>>>>>>>>..<<.<...<<<<<<.....<.<<<
<<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<.<<<<
<........>>>>>>>>....>>>>>>>.>...<<<.....>>>......
>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><<.<<<<<
<<....<<<<<<.....<<<...<<<<<<<<<..<<..............
.>>..>>>>>>>>>..>>.>.....>>>>>>..<<<<<<.........>>
>>>>..>>>>>>>..>>..<<<<<<<<<<......>>>>>>>>>>....<
<<......>>>...<<<<.<<<...<<<(((.....<<<<<<........
...<<..<<<<<<<<<<.........<<<.<<....<<.........>>.
...>>.........<<......>>...>>>.....>>>>>>>>>>.>>..
........))).....>>>>>>.>>>...>>>>>>><<<<<......>>>
>>....>>>>>><.<<<<<<...........<<<<<.<<....>>>>>>>
........>>>>>>..>.....>>>>>><......<<.....<<<<<<..
.....>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>
>>>>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>..
.<<<<<........>>>>>))<<<<<..<<<.<....<<<<....<<<<.
.<<<..<<<<<<<<<<.....[[[..>>>>><...<<<<<...<......
............<<<............>>>.....>..>>>>>....><.
.<<<<<....>>>>>...>>>>>>...>>>...>>>>.........>>>>
.....>....>.>>...>>>>>>>>>..>>.....<<......>>>>>>>
.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.........
<.....<<<<<...............>>>>><<<<<<.......>>>>>>
............<<<<<<<<<..<<<.........>>>..<<<<<<<...
..<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<<<..<<<
...<<..<<<<<<..<<<......>>>...>>>>>>..>>...>>>>>>>
>..>>>>><<<<<<.......>>>>>>........<...........<<<
<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..>>.>
>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<
<<<.<<<..<......<<<<.<<<<<<..<<<<<..<<<<<<<..<<.<<
<.<<<.<<<<<...........<<<<<...<<......<<<<<<<<....
>>>>>>>>...>>.............>>>.>>.>>>>>.>>>.>>>.>>.
..<<<<<.<<<..........>>>>>>>>.....>>>>>>>...>>>>>.
.<<<<.....>>>><<<<<<...........>>>>>>..<<...<<<<<<
<..<<<<<<.........>>>>>>....<<...(((>>....>>>>>>>.
..<<<<..<<<......>>>..>>>>.<<<....>>>.>>))).......
<<<<<<<.<<..))))..>>>>>>>>>.............>>>>..>>>>
>>.......<<<<...<<<<<<...<<.......>>...>>>>>>....>
>>.>.......<<<<<.<.<<<<..<<<<<<<<........>>>>>..>>
>.<<<<<.....>>>>>.....>>>>...>...>>>.>>....<<<<<<<
....>>>.>>>>....>...>>>>>>>>>>>....<<<<<<<...<.<<.
.<<<<<<<...............>>>>>>>.<<<<<...<<<<...<<<<
<<<<<<<]]].>>>>>>...>>>>>...>>>>.>>>>>....<<...<<<
<<<...........>>>>>>...>>..>>.>.......>>>>>>>....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5h1s Cryo-EM structure of the large subunit of the spinach chloroplast ribosome.
Resolution3.5 Å
Binding residue
(original residue number in PDB)
R25 G31 C35 K37 G38 R39 A41 R42 G43 I44 I45 T46 H49 R50 G52 H54 K55 R56 L57 Y58 R59 R64 N66 Y79 P81 R83 N84 G95 I142 R146 G148 Q149 L150 A151 R152 L172 P173 S174 R178 N197 R200 L201 G202 R203 A204 G205 K207 R208 W209 L210 K212 R213 P214 V215 V216 R217 G218 V219 V220 M221 N222 P223 V224 D225 H226 G230 G231 E232 G233 R234 P236 I237 G238 R239 K240 P242 T244 P245 W246 Y248 G252 R253 R254 S255 R256 R258 Y261 R269
Binding residue
(residue number reindexed from 1)
R3 G9 C13 K15 G16 R17 A19 R20 G21 I22 I23 T24 H27 R28 G30 H32 K33 R34 L35 Y36 R37 R42 N44 Y57 P59 R61 N62 G73 I120 R124 G126 Q127 L128 A129 R130 L150 P151 S152 R156 N175 R178 L179 G180 R181 A182 G183 K185 R186 W187 L188 K190 R191 P192 V193 V194 R195 G196 V197 V198 M199 N200 P201 V202 D203 H204 G208 G209 E210 G211 R212 P214 I215 G216 R217 K218 P220 T222 P223 W224 Y226 G230 R231 R232 S233 R234 R236 Y239 R247
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome
GO:0016740 transferase activity
GO:0019843 rRNA binding
Biological Process
GO:0002181 cytoplasmic translation
GO:0006412 translation
Cellular Component
GO:0005840 ribosome
GO:0009507 chloroplast
GO:0015934 large ribosomal subunit
GO:0022625 cytosolic large ribosomal subunit
GO:1990904 ribonucleoprotein complex

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Molecular Function

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Biological Process

View graph for
Cellular Component
External links
PDB RCSB:5h1s, PDBe:5h1s, PDBj:5h1s
PDBsum5h1s
PubMed27762343
UniProtP06509|RK2_SPIOL Large ribosomal subunit protein uL2cz/uL2cy (Gene Name=rpl2-A)

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