Structure of PDB 5exf Chain E Binding Site BS01

Receptor Information

>5exf Chain E (length=483) Species: 1104324 (Pyrobaculum ferrireducens)

KVANYINGEFKEPSTGAFQVKTSPVDGSKIAEVPRSGREDAREAIDSAFE
ALKAWANIPAIRRAEYLYKMLEVFRQMKEDFMKILTVEGGGTYRKVWGEV
VFTERLIQNAAELARHYQGRVLQSDSESTISVVFKRSKGVVGVITPWNYP
LSISMKKIAHTLAVGNTVVYKPASDTPVTGWLIAQMVAKAGLPKGVFNLV
IGPGPVVGEEIVTHKRVAHVTFTGESSTGREIAAKAAGTLKTVTLELGGS
DPLIILDDVDVDYAARLAVFASLFHQGQICTSAKRIIVHKAVADKFIERY
VHYVKMLRIDDPRKDEKVDLGPLINERQVALMKEFVDDAVSRGGRLLIGG
RSWGNFFEPAIFVDVDRNFRIMREEVFGPVRPIVVVENDDQAVEVANDTD
YGLSGAVLTNNVNRAFRIAEAVESGMFHINDVTFLEESHVPFGGIKASGV
GREGGEWSFHETTYDRWVTVTLRTRRFPIPSAL

Ligand information

• Ligand ID: NAP
• InChI: InChI=1S/C21H28N7O17P3/c22-17-12-19(25-7-24-17)28(8-26-12)21-16(44-46(33,34)35)14(30)11(43-21)6-41-48(38,39)45-47(36,37)40-5-10-13(29)15(31)20(42-10)27-3-1-2-9(4-27)18(23)32/h1-4,7-8,10-11,13-16,20-21,29-31H,5-6H2,(H7-,22,23,24,25,32,33,34,35,36,37,38,39)/t10-,11-,13-,14-,15-,16-,20-,21-/m1/s1
• InChIKey: XJLXINKUBYWONI-NNYOXOHSSA-N
• SMILES:
  OpenEye OEToolkits 1.5.0: c1cc(c[n+](c1)C2C(C(C(O2)COP(=O)([O-])OP(=O)(O)OCC3C(C(C(O3)n4cnc5c4ncnc5N)OP(=O)(O)O)O)O)O)C(=O)N
  CACTVS 3.341: NC(=O)c1ccc[n+](c1)[CH]2O[CH](CO[P]([O-])(=O)O[P](O)(=O)OC[CH]3O[CH]([CH](O[P](O)(O)=O)[CH]3O)n4cnc5c(N)ncnc45)[CH](O)[CH]2O
  CACTVS 3.341: NC(=O)c1ccc[n+](c1)[C@@H]2O[C@H](CO[P]([O-])(=O)O[P@@](O)(=O)OC[C@H]3O[C@H]([C@H](O[P](O)(O)=O)[C@@H]3O)n4cnc5c(N)ncnc45)[C@@H](O)[C@H]2O
  OpenEye OEToolkits 1.5.0: c1cc(c[n+](c1)[C@H]2[C@@H]([C@@H]([C@H](O2)CO[P@@](=O)([O-])O[P@](=O)(O)OC[C@@H]3[C@H]([C@H]([C@@H](O3)n4cnc5c4ncnc5N)OP(=O)(O)O)O)O)O)C(=O)N
• Formula: C21 H28 N7 O17 P3
• Name: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE;
  2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE
• ChEMBL: CHEMBL295069
• DrugBank: DB03461
• PDB chain: 5exf Chain E Residue 500

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5exf NADP-Dependent Aldehyde Dehydrogenase from ArchaeonPyrobaculum sp.1860: Structural and Functional Features.
• Resolution: 2.19 Å
• Binding residue (original residue number in PDB): I151 T152 P153 W154 N155 I160 K178 P179 A180 S181 P210 G211 G215 F229 T230 G231 E232 T235 I239 E253 L254 C287 E382 F384
• Binding residue (residue number reindexed from 1): I144 T145 P146 W147 N148 I153 K171 P172 A173 S174 P203 G204 G208 F222 T223 G224 E225 T228 I232 E246 L247 C280 E375 F377
• Annotation score: 4

Enzymatic activity

• Catalytic site (original residue number in PDB): N155 K178 E253 C287 E382 E460
• Catalytic site (residue number reindexed from 1): N148 K171 E246 C280 E375 E453

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity
• GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor

External links

• PDB: RCSB:5exf, PDBe:5exf, PDBj:5exf
• PDBsum: 5exf
• PubMed: 27956891
• UniProt: G7VCG0