Structure of PDB 5an9 Chain E Binding Site BS01

Receptor Information

>5an9 Chain E (length=136) Species: 44689 (Dictyostelium discoideum)

MSKAQAVGSNYRVSLGLPVGAVMNSADNSGAKNLYVIAVKGIKGRLNRLP
SAGVGDMVMATVKKGKPELRKKVCTGLVVRQRKHWKRKDGVYIYFEDNAG
VMCNPKGEVKGNILGPVAKECSDLWPKVATNAGTIV

Ligand information

>5an9 Chain N (length=1162)

auuagcaguuucgggggcguaaugcucucagcugauucucaaacucugaa
cauuuuugguaagcagaacuggcgauguggguugaaccaaauauugggau
aagacgucuaacauucacuaauagauaccacaaaagguguuaguucauua
agacagcaggacgguggccauggaagucgguauccgcuaaggagugugua
acaacucaccugccaaauggacuagcccugaaaauggaugacgcuagcag
uggauggucgaugcccaaucguuaaaagaagugauaauacuuuuaacaau
ccaacuguuuaauuaaaacaaagcauugcgauaagcuuaaaagcuuuuga
cgcaaugugauuucugcccagugcucugaaugucaaagugaagagauuca
accuagcacggguaaacggcgggaguaacuaugacucucuuaagguagcc
aaaugccucgucaucuaauuagugacgcgcaugaauggaucaaugagauu
cccacugucccuaacuacuauacagcgaaaccacugcaaggggaacgggc
cuugcaaaaacagcggggaaagaagacccuguugagcuugacucuagucu
gauauuaacaguuucagguggggaguuuggcuggggcggcacauuuguua
aaagauaacgcaaguguccaaaggcaggcucagugagaacagaaaucuca
cguagaguaaaagggcaaaagccugcuugauucugauuuucaguacuaau
cggaacugggaaaccagggccuaucgauccuuuaugugcuuaaaucuuaa
cccuagaggugucagaaaaguuaccacagggauaacuggcuuguggcagc
caagcgcucauagcgacgcugcuuuuugauccuucgaugucggcucuucu
uaucauugugaagcagaauucacaaaguguuggauuguucacccacuaac
aaggaacgugagcuggguuuagaccgucgugagacagguuaguuuuaccc
uacuguugucaauuguuugcguaauaguagcaugauuuaguacgagagga
acugucaugccggaucacuggucuguagguuuauuugacaaaauagugac
cugccgcuaccauccguuggauaauggcugaacgccucuaagucagaauc
cauucuagaaac

.....<<<<...<<<<<<.....>>>>>>.>>>>................
<<.<<<<<.....>>>>>.>>......<<<......>>>...<<<<<<..
..<<<(((...<<<<<<<....<<..........<<<.<<<<<<<<<<..
.....<<<<<.<<<..<<<.........>>>..>>>.........<<...
...>>...>>>>>...>>>>>>>>>>>>>..........))).>>....>
>>>>>>>>>....>>>>>>.<<<<<<<<...........>>>>>>>><<<
<<<..<<<.........>>>..<<<<<<<....<<<<....>>>>.....
.>>>>>>>...<<..<<<..<<<<<.....<<<.....<<<<....>>>>
.....>>>..>>>..>>>>><<<<<<.......>>>>>>........<..
.........<<<<<.......>>>>>.....>.....>>....>>.>>>>
....................................<<<<<........>
>>>>....<<<<<..<<<.<<......<<<<.<<<<<<..<<<<<.<<<<
<<.<<.....>>.>>>>>>..>>>>>...<<<.....>>>..<<<<<<..
.........>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>
>.....<<...(((>>....>>>>>>>...<<<<<<<<.........>>>
>>>>>.<<<....>>>.>>)))......<<<<<<...<.<<.......>>
.>.>>>>>>.........>>>>..>>>>>>.......<<.<.<.<<<<<<
...<<.......>>...>>>>>>...>>.>.>.......<<<<<.<<<<<
<..<<<<<<<<<......>>>>>>..>>>.<<<.<.....>.>>>.....
>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>
>.>>>>>.............<<..<<<..<<<<<<<..............
...>>>>>>><<<<<...<<<<....<<<<<<<<<......>>>>..>>>
>>...>>>>..>>>>>.<<<<...<<<<<...........>>>>>...>>
>>..>>>...>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5an9 Mechanism of Eif6 Release from the Nascent 60S Ribosomal Subunit
• Resolution: 3.3 Å
• Binding residue (original residue number in PDB): A4 Q5 V7 G8 N10 Y11 R12 S14 I37 K40 G41 I42 K43 G44 R45 L46 N47 R48 L49 M59 T61 K71 K83 H84 K86 Y92
• Binding residue (residue number reindexed from 1): A4 Q5 V7 G8 N10 Y11 R12 S14 I37 K40 G41 I42 K43 G44 R45 L46 N47 R48 L49 M59 T61 K71 K83 H84 K86 Y92

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0031012 extracellular matrix
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5an9, PDBe:5an9, PDBj:5an9
• PDBsum: 5an9
• PubMed: 26479198
• UniProt: Q54G86|RL23_DICDI Large ribosomal subunit protein uL14 (Gene Name=rpl23)