Structure of PDB 4wfb Chain E Binding Site BS01

Receptor Information

>4wfb Chain E (length=156) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

TFDGNHVTVKGPKGELSRTLNERMTFKQEENTIEVVRPSDSKEDRTNHGT
TRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKDLILNVGYSHPVEIKA
EENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGKGIRYQGE
YVRRKE

Ligand information

>4wfb Chain X (length=2707)

gauuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccaau
gaaggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuu
gauccagagauuuccgaauggggaaacccagcaugaguuaugucauguua
ucgauauguauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuaugaguuacaaaggacgacauuagacgaaucaucuggaaag
augaaucaaagaagguaauaauccuguagucgaaaauguugucucucuug
aguggauccugaguacgacggagcacgugaaauuccgucggaaucuggga
ggaccaucuccuaaggcuaaauacucucuagugaccgauagugaaccagu
accgugagggaaaggugaaaagcaccccggaaggggagugaaauagaacc
ugaaaccgugugcuuacaaguagucagagcccguuaaugggugauggcgu
gccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaagca
guaaauguggagccguagcgaaagcgagucugaauagggcguuuaguauu
uggucguagacccgaaaccaggugaucuacccuuggucagguugaaguuc
aggcugaauggaggaccgaaccgacuuacguugaaaagugagcggaugaa
cugaggguagcggagaaauuccaaucgaaccuggagauagcugguucucu
ccgaaauagcuuuagggcuagccucaagugaugauuauuggagguagagc
acuguuuggacgaggggguuaccgaauucagacaaacuccgaaugccaau
aauuuaacuugggagucagaacaugggugauaagguccguguucgaaagg
gaaacagcccagaccaccagcuaaggucccaaaauauauguuaaguggaa
aaggauguggcguugcccagacaacuagcucacuagucggugacacugcg
ccgaaaauguaccggggcuaaacauauuaccgaagcuguggauuguccug
gacaaugguaggagagcguucuaagggcguugaagcaugaucguaaggac
auguggagcgcuuagaagugagaaugccggugugaguagcgaaagacggg
ugagaaucccguccaccgauugacuaagguuuccagaggaaggcucgucc
gcucuggguuagucggguccuaagcugaggccgacaggcguaggcgaugg
auaacagguugauauuccuguaccaccuauaaucguaucgauggggggac
gcaguaggauaggcgagcgugcgauuggaugcacgucuaagcaguaaggc
ugaguaaaauccgguacucgggcugagcugugaugggagaagaagucguu
gauuucacacugccgagaaaagccucuagauagaauaggugcccguaccg
caaaccgacacagguagucaagaugagaauucuaaggugagcgagcgaac
ucucguuaaggaacucggcaaaaugaccccguaacuucgggagaaggggu
gcucuuuaggguuaacgcccagaagagccgcagugaauaggcccaagcga
cuguuuaucaaaaacacaggucucugcuaaaccguaaggugauguauagg
ggcugacgccugcccgugcuggaagguuaagaggagugguuaggcgaagc
uacgaaucgaagccccaguaaacggcggccccuaagguagcgaaauuccu
ugucggguaaguuccgacccgcacgaaaggcguaacgauuugggcacugu
cucaacgagagacucggugaaaucauaguaccugugaagaugcagguuac
ccgcgacaggacggaaagaccccguggagcuuuacuguagccugauauug
aaauucggcuuucuaacccgcaccacuuaucguggugggagacaguguca
agcgggcaguuugacuggggcggucgccuccuaaaagguaacggaggcgc
ucaaagguucccucagaaugguugaaaucauucauagaguguaaaggcau
aagggagcuugacugcgagaccuacaagucgagcagggucgaaagacgga
cuuagugauccggugguuccgcauggaagggccaucgcucaacggauaaa
agcuaccccggggauaacaggcuuaucucccccaagaguucacaucgacg
gggagguuuggcaccucgaugucggcucaucgcauccuggggcuguaguc
ggucccaaggguugggcuguucgcccauuaaagcgguacgcgagcugggu
ucagaacgucgugagacaguucggucccuauccgucgugggcguaggaaa
uuugagaggagcuguccuuaguacgagaggaccgggauggacauaccucu
gguguaccaguugucgugccaacggcauagcuggguagcuauguguggac
gggauaagugcugaaagcaucuaagcaugaagccccccucaagaugagau
uucccaacuuguuauaagaucccucaaagaugaugagguuaauagguucg
agguggaagcauggugacauguggagcugacgaauacuaaucgaucgaag
acuuaau

..<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>...
..>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.
<<<<<<<<<.......>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>.......
<<<<<<<<...<..........<<<<<<<<<.<<<...<<<<<......>
>>>>((......<<<.....))>>>...>>>...>>>>>>>>.>...>.>
>>>>..>>>......<<<<<<<<.........>>>>>>>>......<<<<
<..<<<<>>>>>..)))......]].>>>>>>>.]]..............
.<<....>>....]].........<<<<....>>>>..............
....>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>..
.<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...<<<
.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>
.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<<<<
<<.>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>....>
>>>>>>>>...<<<.....>>>......>>>>>>......>.>>..<<<<
<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.....<<<.
..<<<<<<<<...<<......>>...>>>>>>>>..>>.>.....>>>>>
>>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>>>>>>....<<
<......>>>...<<<<.<<<...<<<(((.....<<<<<<.........
..<<..<<<<.<<<<<.......<<<<......>>>>..>>>>>.>>>>.
>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<<.>
>>>>>>....>>>>>>..<<<<<<<.<<......<<<<<.<<....>>.>
>>>>...>>.>>>>>>>........>>>>>><......<<....<<<<<<
.......>>>>>><<<<....<<<<<.<<<..<<<<<<...<<.....>>
.>>>>>>....((<<<<.<<.<.<<<...<<<....>>>...>>>..>>>
...<<<<<........>>>>>))<<<<<<<......<<<...<<<<....
<<<<...<<...<...<.<<....{.{...>>.>.<....<<<<<..<<<
<.<<<..........>>>.>.>>>>..>>>>....>.............>
....>>...>>>>.........>>>.>.>>>...>>>>>>>>>>>.>>>.
....<<......>>>>>>>.<<<<....>.>>>..>>>>..<<<<....<
<<<<<<<.<<.........<.....<<<<<.....<..>......>>>>>
<<<<<<<.<<<<....>>>>.>>>>>>>............<<<<<<<<<.
.<<<.........>>>..<<<<<<<.....<<<....>>>.......>>>
>>>>.<<<<<..<<.<<<<<<<..<<<<..<<...<<<<<<......>>>
>>>...>>..>>>>>>>>>..>>>>..............<..........
.<<<<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>..>..
>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<
<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<.<<<<<<.<
<<<.<..>.>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>
.>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>>>..
<<...<<<<<<<...<<<<<<......>>>>>>.....<<.[.(((>>..
..>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>>>.>>
)))]......<<<<<<<.((..>>>>..))>>>>>>>.............
>>>>..]]>>>>.......<<<<.<.<<<<<<...<<.......>>...>
>>>>>...>>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......
>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>.
...<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<
<<...<<<<..<<<<<<<...............>>>>>>>.<<<<<...<
<<<...<<<<<<<<<<<.}.}>>>>>>..>>>>>...>>>>.>>>>>...
<<<....<<<<<...........>>>>>....>>>.>>>>.......>>>
>>>>.<<<..>>>....<<<<<<<<<.......>>>>>....<<<<<<<<
.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>....
>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4wfb Structural insights into species-specific features of the ribosome from the pathogen Staphylococcus aureus.
• Resolution: 3.43 Å
• Binding residue (original residue number in PDB): T63 G66 T67 A70 N74 Y109 S110 H111 E139 G142 A143 Y157
• Binding residue (residue number reindexed from 1): T46 G49 T50 A53 N57 Y92 S93 H94 E122 G125 A126 Y140

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4wfb, PDBe:4wfb, PDBj:4wfb
• PDBsum: 4wfb
• PubMed: 26464510
• UniProt: Q2FW21|RL6_STAA8 Large ribosomal subunit protein uL6 (Gene Name=rplF)