Structure of PDB 4k4s Chain E Binding Site BS01

Receptor Information

>4k4s Chain E (length=462) Species: 12081 (Human poliovirus 1 Mahoney)

GEIQWMRPSKEVGYPIINAPSKTKLEPSAFHYVFEGVKEPAVLTKNDPRL
KTDFEEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDA
MYGTDGLEALDLSTSAGYPYVAMGKKKRDILNKQTRDTKEMQKLLDTYGI
NLPLVTYVKDELRSKTKVEQGKSRLIEASSLNDSVAMRMAFGNLYAAFHK
NPGVITGSAVGCDPDLFWSKIPVLMEEKLFAFDYTGYDASLSPAWFEALK
MVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTSIFNSMI
NNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDY
GLTMTPADKSATFETVTWENVTFLKRFFRADEKYPFLIHPVMPMKEIHES
IRWTKDPRNTQDHVRSLCLLAWHNGEEEYNKFLAKIRSVPIGRALDLPEY
STLYRRWLDSFG

Ligand information

>4k4s Chain F (length=17)

uccaggucucucgcgaa

.................

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4k4s Structures of coxsackievirus, rhinovirus, and poliovirus polymerase elongation complexes solved by engineering RNA mediated crystal contacts.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): N18 P20 K24 E108 T114 S115 K127 Y157 K159 I176 E177 A178 R188 H199 G211 C212 D213 S288 G289 C290 S291 L419
• Binding residue (residue number reindexed from 1): N18 P20 K24 E108 T114 S115 K127 Y157 K159 I176 E177 A178 R188 H199 G211 C212 D213 S288 G289 C290 S291 L419

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.4.22.28: picornain 3C.
  3.4.22.29: picornain 2A.
  3.6.1.15: nucleoside-triphosphate phosphatase.

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:4k4s, PDBe:4k4s, PDBj:4k4s
• PDBsum: 4k4s
• PubMed: 23667424
• UniProt: P03300|POLG_POL1M Genome polyprotein