Structure of PDB 7ajt Chain DX Binding Site BS01

Receptor Information

>7ajt Chain DX (length=103) Species: 559292 (Saccharomyces cerevisiae S288C)

PFGGSSHAKGIVLEKLGIESKQPNSAIRKCVRVQLIKNGKKVTAFVPNDG
CLNFVDENDEVLLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKE
KPR

Ligand information

>7ajt Chain D3 (length=1327)

aagauaguuaucugguugauccugccagucauaugcuugucucaaagauu
aagccaugcaugucuaaguauaagcaauuuauacagugaaacugcgaaug
gcucauuaaaucaguuaucguuuauuugauagcaugguauaacuguggua
auucuagagcuaauacaugcuaucucgacccuuuggaagagauuauuuau
uagauucuucggacucuugaugauucauaauaacuuuucgaaucgcaugg
ccuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgau
gguaggauaguggccuaccaugguuucaacggguaacggggaauaagggu
ucgauuccggagagggagccugagaaacggcuaccacauccaaggaaggc
agcaggcgcgcaaauuacccaauccuaauucagggagguagugacaauaa
auaacgauacagggcccauucgggucuuguaauuggaaugaguacaaugu
aaauaccuuaacgaggaacaauuggagggcaagucuggugccagcagccg
cgguaauuccagcuccaauagcguauauugcucguaguugaacuuugggc
ccgguuggccggucggauuuccaacggggccuuuuuacuuugaaaaaauu
agaguguucaaagcaggcguauugcucgauauauuagcauggaauaauag
gacguuugguucuauuuuguugguuucuaggaccaucguaauuaauaggg
acggucgggggcaucaguauucaauugucagaggugaaauucuuggauuu
auugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaau
caagaacgaaacuaugccgacuagggaucgggugguguuuuuuuaaugac
ccacucggcaccuuacgaggaguauggucgcaaggcugaaacuuaaagga
auugacggaagggcaccaccaggaguggagccugcggaaacucaccaggu
ccagacacaauauuugugggugguggugcaugugaugcccuuguucuggc
gcgcgcuacacugacggagccagcgagucuaaccuuggccgagaggucuu
gguaaaaacuccgucggggaacgaggaauuccuaguaagcgcaagucauc
agcuugcguugauuacgucccugcccuuuguacacaccgcccgucgcuag
uaccgauugaauggcuuagugaggccucaggaucugcuuagagaaggggg
caacuccaucucagagcggaaauuuggacaaacuuggucauuuagaggaa
cuaaguuuccguaggugaaccugcgga

...........................<<.<<<<<<...<.<........
..<<<.<<<..<<....<<....<<..........>>...>>.>>.....
.<<..<.....<<<..<<..<<....<<<.......<<....<<.<<<..
...>>>.>>.....>>.....<<<<<..<<....>>..>>>>>...<<<<
<<...<<....>>....<<......>>>>>>>>.........<<<<..<<
<.....>>>.>>>>....>>>...>>>>..>>>.<<<.....<......<
<<<<<<.......>>>>>>>..>.......>>>...<<<.<<<......>
>>....>>>>>>.<<.<<<<........>>>>.>>.<.<......>.>..
.>>>>>>.........<<<....<<<.....>>>..>>>.......>.>.
....<<<<<<<.<<<<<....>>>>>.>>>.>>>>......<<..<....
.......>..>>.........<<<<<<.......<<<....>>>......
............>>>>>>..>>>>>>>>.<.<<...<<<.<<....<<<<
<<.<<<<<............>>>>>.>>>>>>..<<<<<.<<........
...<<.......>>..<<.....>>............>>.>>..>>>...
.<<<....<..<<...............>>..>...>>>..>>....<<<
<<<.<<...<<<<..<<..<<<<<<.<...<<<......>>>......>.
>>>>>>..>>.......<<....>>...>>>>...>>>>>.>>>...>>>
...>>.>..........<<...<<<..<<<<<<<<.<<<........>>>
>>>>>>>>..>>>..>>..................<<.........>>..
...<<<<<<<<<<<<..<<.<<<<<...<<<.<<<<........<<<<<.
.....<<<<<...>>>>>......<<...........>>.....>>>>>>
>>>.>>>.....<<<<<<<............<<<..<<<<....>>>>..
>>>.....>>>>>>>.............>>>>>....<<<<<<<<.....
..>>>>>>>>......>>...>>>>>>>............>>>>>..<<<
<.<<....<<<<<<<<.<<...<.<<......<<<<<<<....<.<.<..
....>.>.>....>>>>>>>.....>>.>...>>.>>>>>>>>...>>.>
>>>....<<<<<<<<....>>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ajt Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome.
• Resolution: 4.6 Å
• Binding residue (original residue number in PDB): S47 H48 Q63 P64 G106 K114 S131 K137
• Binding residue (residue number reindexed from 1): S6 H7 Q22 P23 G65 K73 S90 K96

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation
• GO:0006450 regulation of translational fidelity
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7ajt, PDBe:7ajt, PDBj:7ajt
• PDBsum: 7ajt
• PubMed: 33326748
• UniProt: P0CX29|RS23A_YEAST Small ribosomal subunit protein uS12A (Gene Name=RPS23A)