Structure of PDB 5it8 Chain DP Binding Site BS01

Receptor Information

>5it8 Chain DP (length=117) Species: 83333 (Escherichia coli K-12)

MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNGSEVLVA
ASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYH
GRVQALADAAREAGLQF

Ligand information

>5it8 Chain DB (length=120)

ugccuggcggccguagcgcgguggucccaccugaccccaugccgaacuca
gaagugaaacgccguagcgccgaugguaguguggggucuccccaugcgag
aguagggaacugccaggcau

<<<<<<<<<<.....<<<<<<<<....<<<<<<<.............>>>
>..>>>...>>>>>>.>>.<<.......<<<<<<<<...>>>>>>>>...
....>>...>>>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5it8 Resistance mutations generate divergent antibiotic susceptibility profiles against translation inhibitors.
• Resolution: 3.12 Å
• Binding residue (original residue number in PDB): K3 K4 R15 R25 R30 T31 P32 R33 H34 Y36 Q38 I40 G44 S45 E46 V47 V54 K63 Y64 N67 K68 Q98 H100 G101 R102
• Binding residue (residue number reindexed from 1): K3 K4 R15 R25 R30 T31 P32 R33 H34 Y36 Q38 I40 G44 S45 E46 V47 V54 K63 Y64 N67 K68 Q98 H100 G101 R102

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5it8, PDBe:5it8, PDBj:5it8
• PDBsum: 5it8
• PubMed: 27382179
• UniProt: P0C018|RL18_ECOLI Large ribosomal subunit protein uL18 (Gene Name=rplR)