Structure of PDB 4v6c Chain DL Binding Site BS01

Receptor Information

>4v6c Chain DL (length=143) Species: 83333 (Escherichia coli K-12)

RLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGGGVRRGFE
GGQMPLYRRLPKFGFTSRKAAITAEIRLSDLAKVEGGVVDLNTLKAANII
GIQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE

Ligand information

>4v6c Chain DA (length=2841)

gguuaagcgacuaagcguacacgguggaugcccuggcagucagaggcgau
gaaggacgugcuaaucugcgauaagcgucgguaaggugauaugaaccguu
auaaccggcgauuuccgaauggggaaacccaguguguuucgacacacuau
cauuaacugaauccauagguuaaugaggcgaaccgggggaacugaaacau
cuaaguaccccgaggaaaagaaaucaaccgagauucccccaguagcggcg
agcgaacggggagcagcccagagccugaaucaguguguguguuaguggaa
gcgucuggaaaggcgcgcgauacagggugacagccccguacacaaaaaug
cacaugcugugagcucgaugaguagggcgggacacgugguauccugucug
aauauggggggaccauccuccaaggcuaaauacuccugacugaccgauag
ugaaccaguaccgugagggaaaggcgaaaagaaccccggcgaggggagug
aaaaagaaccugaaaccguguacguacaagcagugggagcacgcuuaggc
gugugacugcguaccuuuuguauaaugggucagcgacuuauauucuguag
caagguuaaccgaauaggggagccgaagggaaaccgagucuuaacugggc
guuaaguugcaggguauagacccgaaacccggugaucuagccaugggcag
guugaagguuggguaacacuaacuggaggaccgaaccgacuaauguugaa
aaauuagcggaugacuuguggcugggggugaaaggccaaucaaaccggga
gauagcugguucuccccgaaagcuauuuagguagcgccucgugaauucau
cuccggggguagagcacuguuucggcaacaacccgaugcaaacugcgaau
accggagaauguuaucacgggagacacacggcgggugcuaacguccgucg
ugaagagggaaacaacccagaccgccagcuaaggucccaaagucaugguu
aagugggaaacgaugugggaaggcccagacagccaggauguuggcuuaga
agcagccaucauuuaaagaaagcguaauagcucacuggucgagucggccu
gcgcggaagauguaacggggcuaaaccaugcaccgaagcugcggcagcga
cgcuuaugcguuguuggguaggggagcguucuguaagccugcgaaggugu
gcugugaggcaugcuggagguaucagaagugcgaaugcugacauaaguaa
cgauaaagcgggugaaaagcccgcucgccggaagaccaaggguuccuguc
caacguuaaucggggcagggugagucgaccccuaaggcgaggccgaaagg
cguagucgaugggaaacagguuaauauuccuguacuugguguuacugcga
aggggggacggagaaggcuauguuggccgggcgacgguugucccgguuua
agcguguaggcugguuuuccaggcaaauccggaaaaucaaggcugaggcg
ugaugacgaggcacuacggugcugaagcaacaaaugcccugcuuccagga
aaagccucuaagcaucagguaacaucaaaucguaccccaaaccgacacag
guggucagguagagaauaccaaggcgcuugagagaacucgggugaaggaa
cuaggcaaaauggugccguaacuucgggagaaggcacgcugauauguagg
ugaggucccucgcggauggagcugaaaucagucgaagauaccagcuggcu
gcaacuguuuauuaaaaacacagcacugugcaaacacgaaaguggacgua
uacggugugacgccugcccggugccggaagguuaauugaugggguuagcg
caagcgaagcucuugaucgaagccccgguaaacggcggccguaacuauaa
cgguccuaagguagcgaaauuccuugucggguaaguuccgaccugcacga
auggcguaaugauggccaggcugucuccacccgagacucagugaaauuga
acucgcugugaagaugcaguguacccgcggcaagacggaaagaccccgug
aaccuuuacuauagcuugacacugaacauugagccuugauggaagugugg
acgccagucugcauggcuuuaauguuugauguucuaacguugacccguaa
uccggguugcggacagugucugguggguaguuugacuggggcggucuccu
ccuaaagaguaacggaggagcacgaagguuggcuaauccuggucggacau
caggagguuagugcaauggcauaagccagcuugacugcgagcgugacggc
gcgagcaggugcgaaagcaggucauagugauccggugguucugaauggaa
gggccaucgcucaacggauaaaagguacuccggggauaacaggcugauac
cgcccaagaguucauaucgacggcgguguuuggcaccucgaugucggcuc
aucacauccuggggcugaaguaggucccaaggguauggcuguucgccauu
uaaagugguacgcgagcuggguuuagaacgucgugagacaguucgguccc
uaucugccgugggcgcuggagaacugaggggggcugcuccuaguacgaga
ggaccggaguggacgcaucacugguguucggguugucaugccaauggcac
ugcccgguagcuaaaugcggaagagauaagugcugaaagcaucuaagcac
gaaacuugccccgagaugaguucucccugacccuuuaaggguccugaagg
aacguugaagacgacgacguugauaggccggguguguaagcgcagcgaug
cguugagcuaaccgguacuaaugaaccgugaggcuuaaccu

<<<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<...
...<<<<<<.[..(((.>>>.....<<<<<<<..<<<)))..].>>>...
...>>>>>>>...>>>....<<<....>>><<<<<<<....>>>>>>>.<
<<<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<......
>......>>>>>.>.........<<....>>..<<<<<<.....<<....
.>>....>>>>>>..(((..<<<<......<<<<<<<<<<<<<.<<<...
<<<<........>>>>((......<<<.....))>>>...>>>...>>>>
>>>>>>>>>...>>>>........<<<<<<<<.........>>>>>>>>.
.....<<<<<..<<<<>>>>>..)))......]].>>>>>>>.]].....
..........<<....>>....]].........<<<<.....>>>>....
..............>>>>>>>>>>>.......<<<<..<<<<<......>
>>>>..>>>>...<............>.<<<<<<....<<<<<<<<<<<<
<..<<<...<<.....>>...>>>...<<....>>..<<<<.....>>>>
.....>>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<
<<<...<<<<<<......>>>>>>.>...>>>...<<<.<<<<<......
..>>>>>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>.
.....>.>>..<<<<<<....<<<<<....>>>>>.<<<<<<<<...<<<
<<<<<<.....<<<...<<<.<<<<.......>>>>.>>>..>>.>....
.>>>>>>.>>>...>>>>>>>.>...<<<<<<<<<<......>>>>>>>>
>>....<<<......>>>...<<<<.<<<...<<<(((.....<<<<<<.
..........<<..<<<<<..<<........<<<<<.<<<..<<<.....
....>>>..>>>........<<<......>>>..>>>>>....>>..>>>
>>.>>...........))).....>>>>>>.>>>...>>>>>>><<<<<<
<<<....>>>>>>>>>....>>>>>><.<<<<<...<<<......<<<<<
<<<....>>>>>>>>...>>>..>>>>>..>.....>>>>>><......<
<.....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<
...<<.....>>.>>>>>>....((<<<.<<<...<<<...<<<....>>
>...>>>...>>>..<<<<<........>>>>>))<<<<<<<<<<<<<..
.<<<<....<<<<..<<<..<<<<<<<<<<<....(((...>>>>>><<.
.<<<<...<<<<<<<<<<<<.<<.....>>>>>>>>>>..>>>>...>>>
>...>><..<<<<<....>>>>>...>>>>>>...>>>...>>>>.....
....>>>>...>>....>>>>>>>>>>>.>>>..>>.....<<......>
>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<...
......<.....<<<<<...............>>>>><<<<<<.....<<
<...<<<<.....>>>>...>>>...>>>>>>............<<<<<<
<<<..<<<.........>>>..<<<<<<<.....<<<....>>>......
.>>>>>>>.<<<<<.<<<..<<<<<<<..<<<<.......<<<<<..<<.
...>>...>>>>>.......>>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<<.....>>>>>>.....>..
..>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>..
...<<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[
[<<<<..<<<<..<<<.<<<<<.<<<<<<<<<<<.<<<<.....<<<<<<
<......>>>>>>>....>>>>.>>>>>>>>>>>...<<<.<<<<<<...
..>>>>>>>>>....>>>>>.>>>...>>>>..<<<<.....>>>><<<<
<<...........>>>>>>..<<...<<<<<<<...<<<<<<.......>
>>>>>.....<<...(((>>....>>>>>>>...<<<<..<<<<....>>
>>..>>>>.<<<....>>>.>>))).......<<<<<<<.((..>>>>..
))>>>>>>>.............>>>>..]]>>>>.......<<<<...<<
<<<<...<<.......>>...>>>>>>....>>>.>.......<<<<<.<
<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.
....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>....]]
..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<..........
.....>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<))).>>>>>>.
.>>>>>...>>>>.>>>>>...<<<....<<<<<...........>>>>>
....>>>.>>>>.......>>>>>>>..<<<<<<...>>>>>><....<<
..<<<<<.......>>>>>....<<<<<<<<..<<....<<<<<....>>
>>>...>>...>>>>>.>>>.....>>..>..>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v6c Structures of the ribosome in intermediate States of ratcheting.
• Resolution: 3.19 Å
• Binding residue (original residue number in PDB): S7 P8 S12 K13 K14 A15 K17 R18 G20 R21 G22 G24 S25 G28 K29 T30 G32 R33 G34 H35 K36 G37 Q38 K39 R41 S42 G43 R47 R48 F50 E51 G52 Q54 M55 Y58 R59 R60 L61 P62 K63 F64 G65 F66 T67 S68 R69 K70 E76 R78 K109 I111 L112 T128 K129
• Binding residue (residue number reindexed from 1): S6 P7 S11 K12 K13 A14 K16 R17 G19 R20 G21 G23 S24 G27 K28 T29 G31 R32 G33 H34 K35 G36 Q37 K38 R40 S41 G42 R46 R47 F49 E50 G51 Q53 M54 Y57 R58 R59 L60 P61 K62 F63 G64 F65 T66 S67 R68 K69 E75 R77 K108 I110 L111 T127 K128

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v6c, PDBe:4v6c, PDBj:4v6c
• PDBsum: 4v6c
• PubMed: 19696352
• UniProt: P02413|RL15_ECOLI Large ribosomal subunit protein uL15 (Gene Name=rplO)