Structure of PDB 7aju Chain DG Binding Site BS01

Receptor Information

>7aju Chain DG (length=218) Species: 559292 (Saccharomyces cerevisiae S288C)

MKLNISYPVNGSQKTFEIDDEHRIRVFFDKRIGQEVDGEAVGDEFKGYVF
KISGGNDKQGFPMKQGVLLPTRIKLLLTKNVSCYRPRRDGERKRKSVRGA
IVGPDLAVLALVIVKKGEQELEGLTDTTVPKRLGPKRANNIRKFFGLSKE
DDVRDFVIRREVTKGEKTYTKAPKIQRLVTPQRLQRKRHQRALKVRNAQA
QREAAAEYAQLLAKRLSE

Ligand information

>7aju Chain D3 (length=1409)

ucugguugauccugccaguagucauaugcuugucucaaagauuaagccau
gcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcucauu
aaaucaguuaucguuuauuugauaguuccuuacaugguauaacuguggua
auucuagagcuaauacaugcuaucucgacccuuuggaagagauguauuua
uuagauaucuucggacugaugauucauaauaacuuuucgaaucgcauggc
cuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgaug
guaggauaguggccuaccaugguuucaacggguaacggggaauaaggguu
cgauuccggagagggagccugagaaacggcuaccacauccaaggaaggca
gcaggcgcgcaaauuacccaauccuaauucagggagguagugacaauaaa
uaacgauacagggcccauucgggucuuguaauuggaaugaguacaaugua
aauaccuuaacgaggaacaauuggagggcaagucuggugccagcagccgc
gguaauuccagcuccaauagcguauauuguuguugcaguuaaaaagcucg
uaguugaacuggcccgguuggccggucggauuuccaacggggccuuuccu
ucuggcuaaccuugaguccuuguggcucuuggcgaaccaguuacuuugaa
aaaauuagaguguucaaagcaggcguauugcucgauauauuagcauggaa
uaauaguaggagguucuauucuaggaccaucguauuaauagggacggucg
ggggcaucaguauucaauugucagaggugaaauucuuggauuuauugaag
acuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaagaac
gaaaguuaggggaucgaagaugaucagauaccgucguagucuuaaccaua
aacuaugccgacuagggaucgggugguguuuuuuuaaugacccacucggc
accuuacgagaaaucaaagucuuuggguucugggggaguauggucgcaag
gcugaaaaaggaauugacggaagggcaccaccaggaguggagccugcggc
gcgcuacacugacggagccagcgagaccuuggccgagaggucuugggaaa
cuccgucgugcuggggauagagcauuguaauuauugcucuucaacgagga
auuccuaguaagcgcaagucaucagcuugcguugauuacgucccugcccu
uuguacacaccgcccgucgcuaguaccgauugaauggcuuagugaggccu
caggaucugcuuagagaagggggcaacuccaucucagagcggagauuugg
acaaacuuggucauuugaggaacuaauuccguaggugaaccugcggaagg
aucauuaaa

.<<<<<.......>>>>>.....<<<<<<...<.<..........<<<.<
<<..<<....<<....<<..........>>...>>.>>......<<....
....<<<..<<..<<....<<<..............<.....<<.<<...
....>>.>>......>.....<<<<<..<<....>>..>>>>><...<<<
<<<....<<....>>.<<......>>>>>>>>.....>...<<<<..<<<
.....>>>.>>>>....>>>...>>>>..>>>.<<<....<<<....<<<
<<<<<.......>>>>>>>>>>>......>>>...<<<.<<<<....>>>
>....>>>.>>.<<.<<<..........>>>.>>.<.<<<..>>>.>...
>>>>>>.........<<<....<<<.....>>>..>>>.......>.>..
...<<<<<<<.<<<<<....>>>>>.>>>.>>>>......<<..<.....
......>..>>.........<<<<<<.......<<<....>>>.......
...........>>>>>>..>>>>>>..........<<<((.....<.<<.
..<<<.<<..<<<<<.<<<<<.....<>.....>>>>>.>>>>>...<<<
.<<<<.<..<<..<<<<<.....>>>>>..>>..>.>>>><<<<<.<<..
.....<<..<<.......>>.<<<.....>>>...>>......>>.>>..
>>>....>>>..<<.<<<...>>>.>>.......>>....<<<<<<.<<.
..<<<<..<<..<<<<<<.<...<<<..(...>>>......>.>>>>>>.
.>>.......<<....>>...>>>>...>>>>>.>>>...>>>...>>.>
....<<<<<<<...<...<<<<..)......>>>>...>>>>>>>>....
......<<<.<<.<<<..<<<<<<<<.<<<........>>>>>>>>>>>.
.>>>...<<..))>>...>>.....>>>.>>>..................
...............<<<<<<<<<<<<..<<.<<<<<<..<<<.<<<.>>
>.>>>.....<<<<<<<..........<..<<<<....>>>>..>.....
>>>>>>>...........<<<<<<.<<....>>.>>>>>>..........
>>>>>>....<<<<<<<.........>>>>>>>......>>...>>>>>>
>............>>>>>..<<<<.<<....<<<<<<<<.<<<..<.<<.
.....<<<<<<<..<.<.<<<......>>>.>.>..>>>>>>>.....>>
.>..>>>.>>>>>>>>..>>.>>>>.<<<<<<<<<....>>>>>>>>>..
.........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7aju Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): N4 Q13 T15 S53 N56 K58 Q59 G60 R72 C83 Y84 R87 D89 G90 R92 K93 R94 K95 V108 V112 K131 R132 L133 G134 K136 R137 N139 K149 R154 R159 R160 Y169 K171 A172 P173 K174 I175 Q176 R177 V179 R183 L184 Q185 R186 K187 R188 Q190 K194 V195 N197 A198 Q201 R202
• Binding residue (residue number reindexed from 1): N4 Q13 T15 S53 N56 K58 Q59 G60 R72 C83 Y84 R87 D89 G90 R92 K93 R94 K95 V108 V112 K131 R132 L133 G134 K136 R137 N139 K149 R154 R159 R160 Y169 K171 A172 P173 K174 I175 Q176 R177 V179 R183 L184 Q185 R186 K187 R188 Q190 K194 V195 N197 A198 Q201 R202

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7aju, PDBe:7aju, PDBj:7aju
• PDBsum: 7aju
• PubMed: 33326748
• UniProt: P0CX37|RS6A_YEAST Small ribosomal subunit protein eS6A (Gene Name=RPS6A)