Structure of PDB 4v7l Chain DE Binding Site BS01

Receptor Information

>4v7l Chain DE (length=204) Species: 300852 (Thermus thermophilus HB8)

MKGILGVKVGMTRIFRDDRAVPVTVILAGPCPVVQRRTPEKDGYTAVQLG
FLPQNPKRVNRPLKGHFAKAGVEPVRILREIRDFNPEGDTVTVEIFKPGE
RVDVTGTSKGRGFAGVMKRWNFAGGPDSHGAHKIHRHPGSIGNRKTPGRV
YKGKKMAGHYGAERVTVMNLEVVDVIPEENLLLVKGAVPGPNGGLVIVRE
TKKA

Ligand information

>4v7l Chain DA (length=2800)

agaugguaagggcccacgguggaugccucggcacccgagccgaugaagga
cguggcuaccugcgauaagccagggggagccgguagcgggcguggauccc
uggauguccgaaugggggaacccggccggcgggaacgccggucaccgcgc
uugcgcggggggaaccuggggaacugaaacaucucaguacccagaggaga
ggaaagagaaaucgacucccugaguagcggcgagcgaaaggggaccagcc
uaaaccguccggcuuguccgggcggggucgggggcccucggccgaauccc
cagccuagccgaagcuguugggaagcagcgccagagagggugaaagcccc
guaggcgaaagguggggggauaggugaggguacccgaguaccccgugguu
cguggagccaugggggaaucugggcggaccaccgccuaaggcuaaguacu
ccgggugaccgauagcgcaccaguaccgugagggaaaggugaaaagaacc
ccgggaggggagugaaauagagccugaaaccgugggcuuacaagcaguca
cggccccaagggguuguggcgugccuauugaagcaugagccggcgacuca
cggucgugggcgagcuuaagccguugaggcggaggcguagggaaaccgag
uccgaacagggcgguccgcggccguggacccgaaaccgggcgagcuagcc
cuggccagggugaagcuggggugagacccaguggaggcccgaaccggugg
gggaugcaaaccccucggaugagcuggggcuaggagugaaaagcuaaccg
agcccggagauagcugguucuccccgaaaugacuuuagggucagccucag
gcgcugacuggggccuguagagcacugauagggcuagggggcccaccagc
cuaccaaacccugucaaacuccgaagggucccagguggagccugggagug
agggcgcgagcgauaacguccgcguccgagcgcgggaacaaccgagaccg
ccagcuaaggcccccaagucugggcuaagugguaaaggauguggcgccgc
gaagacagccagucgaguggcgccgcgccgaaaaugaucggggcuuaagc
ccagcgccgaagcugcgggucugggggaugaccccaggcgguaggggagc
guucccgaugccgaugaaggccgacccgcgagggcggcuggagguaaggg
aagugcgaaugccggcaugaguaacgauaaagagggugagaaucccucuc
gccguaagcccaaggguuccuacgcaauggucgucagcguaggguuaggc
gggaccuaaggugaagccgaaaggcguagccgaagggcagccgguuaaua
uuccggcccuucccgcaggugcgauggggggacgcucuaggcuaggggga
ccggagccauggacgagcccggccagaagcgcagggugggagguaggcaa
auccgccucccaacaagcucugcgugguggggaaaaccccccgaagccag
ggagccaagaaaagccucuaagcacaaccugcgggaacccguaccgcaaa
ccgacacaggugggcgggugcaagagcacucaggcgcgcgggagaacccu
cgccaaggaacucugcaaguuggccccguaacuucgggagaaggggugcu
cccuggggugaugagccccggggagccgcagugaacaggcucuggcgacu
guuuaccaaaaacacagcucucugcgaacucguaagaggagguauaggga
gcgacgcuugcccggugccggaaggucaaggggaggggugcaagccccga
accgaagccccggugaacggcggccguaacuauaacgguccuaagguagc
gaaauuccuugucggguaaguuccgaccugcacgaaaagcguaacgaccg
gagcgcugucucggcgagggacccggugaaauugaacuggccgugaagau
gcggccuacccguggcaggacgaaaagaccccguggagcuuuacugcagc
cugguguuggcucuuggucgcgccugcguaggauaggugggagccuguga
acccccgccuccgggugggggggaggcgccggugaaauaccacccuggcg
cggcugggggccuaacccucggauggggggacagcgcuuggcgggcaguu
ugacuggggcggucgccuccuaaaagguaacggaggcgcccaaagguccc
cucaggcgggacggaaauccgccggagagcgcaaggguagaagggggccu
gacugcgaggccugcaagccgagcaggggcgaaagccgggccuagugaac
cgguggucccguguggaagggccaucgaucaacggauaaaaguuaccccg
gggauaacaggcugaucucccccgagcguccacagcggcggggagguuug
gcaccucgaugucggcucgucgcauccuggggcugaagaaggucccaagg
guugggcuguucgcccauuaaagcggcacgcgagcuggguucagaacguc
gugagacaguucggucucuauccgccacgggcgcaggaggcuugaggggg
gcucuuccuaguacgagaggaccggaagggacgcaccucugguuucccag
cugucccuccaggggcauaagcuggguagccaugugcggaagggauaacc
gcugaaagcaucuaagcgggaagcccgccccaagaugaggccucccacgg
cgucaagccgguaaggacccgggaagaccacccgguggaugggccggggg
uguaagcgccgcgaggcguugagccgaccggucccaaucguccgaggucu

<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<......<<<
<<<.[...((.>>>.....<<<<<<<..<<<))...].>>>.....>>>>
>>>...>>>....<<<....>>><<<<<<<<....>>>>>>>>.<<<<<<
..>>>>>>.>>....<<<<<.....<......>......>>>>>......
.....<<....>>...<<<<<.....<<.....>>....>>>>>...<<<
....<<<<<<<<.....>>>>>>>>.....<<<<<<<<...<<...<<<<
..<<<<.<<<...<<<<<......>>>>>((......<<<.....))>>>
...>>>...>>>>...>>>>..>>..>>>>>.>>>.....<<<<<<<<<.
......>>>>>>>>>......<<<<<..((((>>>>>..>>>......>>
.>>>>>>.>>...............<<....>>....>>.........<<
<<....>>>>..................>>>>>>>>>>>.......<<<<
<<<<<<<..>>>>>>>>>>>...<............>.<<<<<<....<<
<<<<<<<<<<...<<<...<<<.....>>>...>>>...<<....>>..<
<<<.....>>>>..>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<
<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<<<<.<<
<<........>>>>>>>>....>>>>>>>>>...<<<.....>>>.....
.>>>>>>.......>>>..<<<<<<....<<<<<....>>>>><<<<<<<
<<.....<<<<<<<<<...<<<...<<<.<<<<...<<<<<........>
>>.>>...>>>>.>>>..>>.>...>>>>>>>>>.....>>>>>>>.>>.
.<<<<<<<.<<......>>.>>>>>>>.....<<<......>>>...<<<
<.<<<...<<<(((.....<<<<<<...........<<..<<<<<<<<<.
.................>>>>>>>>>.>>..........)))......>>
>>>>.>>>...>>>>>>>.<<<<<<.......>>>>>>.....>>>>>><
.<<<<<...<<<......<<<<<.<<<....>>>>>>>>...>>>..>>>
>>..>.....>>>>>><......<<.....<<<<<<.......>>>>>><
<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....((<
<<<.<<...<<<...<<<....>>>...>>>...>>...<<<<<......
..>>>>>))<<<<<<<<<<<<<...<<<<....<<<<<.<<<..<<<<<.
<<<<.<<..[[[....>>>>>><<<...<<<<<<<<<<<<<<<<.<<...
..>>>>>>>>>.....>>>>>>>>>..>>><.....>>>>>>...>>>..
>>>>>.........>>>.>..>>...>>>>>>>>>>>>>>>..>>.....
<<......>>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<<<<
<<<<.<<.........<.....<<<<<.....<..>......>>>>><<<
<<<<<<<<<.....>>>>>>>>>>>>............<<<<<<<<<..<
<<.........>>>..<<<<<<<.....<<<....>>>.......>>>>>
>>.<<<<<.<<<..<<<<<<<..<<<...<<...<<<<<....>>>>>..
.>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>........<.
..........<<<<<.......>>>>>.....>....>>>>>..>>.>>>
>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<.......
.>>>>>..<<<<<<<<.<<<.........<<<<.<<<<<<..<<<<<.<<
<.<<<<<.<<<<<<<<<<<<<<<<...........<<<<<..<<<.....
.<<<<<<<<....>>>>>>>>...>>>............>>>>>..>>>>
>>>>>>>>>>>>...<<<<<....>>>>>....>>>>>.>>>..>>>>>.
.<<<<.....>>>><<<<<<...........>>>>>>..<<...<<<<<<
<...<<<<<<.......>>>>>>.....<....(((.>....>>>>>>>.
..<<<<..<<<......>>>..>>>>.<<<....>>>.>>))).......
<<<<<<<.<<..))))..>>>>>>>>>.............>>>>..>>>>
>>.......<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>
>>.>.......<<<<<.<.<<<<..<<<<<<<<<......>>>>>>>..>
>.<<<<<.....>>>>>.....>>>>...>...>>>.>>....<<<<<<<
....>>>.>>>>........>>>>>>>>>>>....<<<<<<<...<<<<.
.<<<<<<<...............>>>>>>>.<<<<<...<<<<...<<<<
<<<<<<<.]]]>>>>>>...>>>>>...>>>>.>>>>>...<<<....<<
<<.............>>>>....>>>.>>>>.......>>>>>>>...<<
.......>>.....<<<<<<<<<.......>>>>>....<<<<<<<<.<<
<....<<<<<....>>>>>...>>>..>>>>>.>>>...>>>>....>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v7l The structures of the anti-tuberculosis antibiotics viomycin and capreomycin bound to the 70S ribosome.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): M11 T12 R13 Q35 R37 K41 D42 Y44 R58 N60 R61 P62 K64 H66 I77 L78 R79 E80 R82 S108 K109 G110 R111 G112 F113 A114 G115 K118 F122 A123 D127 S128 H129 G130 A131 H132 K133 I134 H135 R136 H137 P138 G139 S140 I141 G142 N143 R144 K145 T146 P147 G148 R149 V150 Y151 K152 K154 M156 A157 H159 R164 T166 M168 L170 K185 A187 V188 P191 N192 K202
• Binding residue (residue number reindexed from 1): M11 T12 R13 Q35 R37 K41 D42 Y44 R58 N60 R61 P62 K64 H66 I77 L78 R79 E80 R82 S108 K109 G110 R111 G112 F113 A114 G115 K118 F122 A123 D127 S128 H129 G130 A131 H132 K133 I134 H135 R136 H137 P138 G139 S140 I141 G142 N143 R144 K145 T146 P147 G148 R149 V150 Y151 K152 K154 M156 A157 H159 R164 T166 M168 L170 K185 A187 V188 P191 N192 K202

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v7l, PDBe:4v7l, PDBj:4v7l
• PDBsum: 4v7l
• PubMed: 20154709
• UniProt: Q5SHN8|RL3_THET8 Large ribosomal subunit protein uL3 (Gene Name=rplC)