Structure of PDB 5juo Chain DC Binding Site BS01

Receptor Information

>5juo Chain DC (length=841) Species: 4932 (Saccharomyces cerevisiae)

VAFTVDQMRSLMDKVTNVRNMSVIAHVDHGKSTLTDSLVQRAGIISAAKA
GEARFTDTRKDEQERGITIKSTAISLYSEMSDEDVKEIKQKTDGNSFLIN
LIDSPGHVDFSSEVTAALRVTDGALVVVDTIEGVCVQTETVLRQALGERI
KPVVVINKVDRALLELQVSKEDLYQTFARTVESVNVIVSTYADEVLGDVQ
VYPARGTVAFGSGLHGWAFTIRQFATRYAKKFGVDKAKMMDRLWGDSFFN
PKTKKWTNKDTDAEGKPLERAFNMFILDPIFRLFTAIMNFKKDEIPVLLE
KLEIVLKGDEKDLEGKALLKVVMRKFLPAADALLEMIVLHLPSPVTAQAY
RAEQLYEGPADDANCIAIKNCDPKADLMLYVSKMVPTSDKGRFYAFGRVF
AGTVKSGQKVRIQGPNYVPGKKDDLFIKAIQRVVLMMGRFVEPIDDCPAG
NIIGLVGIDQFLLKTGTLTTSETAHNMKVMKFSVSPVVQVAVEVKNANDL
PKLVEGLKRLSKSDPCVLTYMSESGEHIVAGTGELHLEICLQDLEHDHAG
VPLKISPPVVAYRETVESESSQTALSKSPNKHNRIYLKAEPIDEEVSLAI
ENGIINPRDDFKARARIMADDYGWDVTDARKIWCFGPDGNGPNLVIDQTK
AVQYLHEIKDSVVAAFQWATKEGPIFGEEMRSVRVNILDVTLHADAIHRG
GGQIIPTMRRATYAGFLLADPKIQEPVFLVEIQCPEQAVGGIYSVLNKKR
GQVVSEEQRPGTPLFTVKAYLPVNESFGFTGELRQATGGQAFPQMVFDHW
STLGSDPLDPTSKAGEIVLAARKRHGMKEEVPGWQEYYDKL

Ligand information

>5juo Chain A (length=1781)

uaucugguugauccugccaguagucauaugcuugucucaaagauuaagcc
augcaugucuaaguauaagcaauuuauacagugaaacugcgaauggcuca
uuaaaucaguuaucguuuauuugauaguuccuuuacuacaugguauaacu
gugguaauucuagagcuaauacaugcuuaaaaucucgacccuuuggaaga
gauguauuuauuagauaaaaaaucaaugucuucggacucuuugaugauuc
auaauaacuuuucgaaucgcauggccuugugcuggcgaugguucauucaa
auuucugcccuaucaacuuucgaugguaggauaguggccuaccaugguuu
caacggguaacggggaauaaggguucgauuccggagagggagccugagaa
acggcuaccacauccaaggaaggcagcaggcgcgcaaauuacccaauccu
aauucagggagguagugacaauaaauaacgauacagggcccauucggguc
uuguaauuggaaugaguacaauguaaauaccuuaacgaggaacaauugga
gggcaagucuggugccagcagccgcgguaauuccagcuccaauagcguau
auuaaaguuguugcaguuaaaaagcucguaguugaacuuugggcccgguu
ggccggucggauuuccaacggggccuuuccuucuggcuaaccuugagucc
uuguggcucuuggcgaaccaggacuuuuacuuugaaaaaauuagaguguu
caaagcaggcguauugcucgaauauauuagcauggaauaauagaauagga
cguuugguucuauuuuguugguuucuaggaccaucguaaugauuaauagg
gacggucgggggcaucaguauucaauugucagaggugaaauucuuggauu
uauugaagacuaacuacugcgaaagcauuugccaaggacguuuucauuaa
ucaagaacgaaaguuaggggaucgaagaugaucagauaccgucguagucu
uaaccauaaacuaugccgacuagggaucgggugguguuuuuuuaaugacc
cacucggcaccuuacgagaaaucaaagucuuuggguucuggggggaguau
ggucgcaaggcugaaacuuaaaggaauugacggaagggcaccaccaggag
uggagccugcggcuuaauuugacucaacacggggaaacucaccaggucca
gacacaauaaggauugacagauugagagcucuuucuugauuuugugggug
guggugcauggccguucuuaguugguggagugauuugucugcuuaauugc
gauaacgaacgagaccuuaaccuacuaaauaguggugcuagcauuugcug
guuauccacuucuuagagggacuaucgguuucaagccgauggaaguuuga
ggcaauaacaggucugugaugcccuuagacguucugggccgcacgcgcgc
uacacugacggagccagcgagucuaaccuuggccgagaggucuugguaau
cuugugaaacuccgucgugcuggggauagagcauuguaauuauugcucuu
caacgaggaauuccuaguaagcgcaagucaucagcuugcguugauuacgu
cccugcccuuuguacacaccgcccgucgcuaguaccgauugaauggcuua
gugaggccucaggaucugcuuagagaagggggcaacuccaucucagagcg
gagaauuuggacaaacuuggucauuuagaggaacuaaaagucguaacaag
guuuccguaggugaaccugcggaaggaucau

...<<<<..{.[[[[.>>>><<<<.<<<<<......<..........<<<
.<<<..<<....<<....<<..........>>...>>.>>......<<..
......<<<..<<..<<....<<<..................<.....<<
..<.......>..>>......>.........<<<<<............>>
>>><...<<<<<<......((((((..<<......>>.............
.>>>>>>.....>...<<<<..<<<.....>>>.>>>>....>>>...>>
>>..>>>.<<<....<<<....<<<<<<<<.......>>>>>>>>>>>..
....>>>...<<<.<<<<....>>>>....>>>.>>.<<.<<<.......
...>>>.>>.<.<<....>>.>...>>>>>>.........<<<....<<<
.....>>>..>>>.......>.......<<.<<<<<..............
>>>>>..>>......<<..<...........>..>>.........<<<<<
((....<.<<<<.....<<..))>>.......>>>>>>>>>>...>>>>>
>>>>.........<<<[[.....<.<<...<<<.<<....<<<<<<.<<<
<.....<..>.....>>>>.>>>>>>.....<<<<<..............
.................>>>>>....<<<<<.<<.......<<...<...
....>..<<<.....>>>....>>......>>.>>..>>>..........
....<.<<<<<.......))))))..>>>>>.>.........>>....<<
.<<<.<<...<<<<..<<<.<<<<<<.....<<<......>>>.......
.>>>>>.>>>>.......<<....>>...>>>>..>>.>>>..>>...>>
>...>>.>....<<.<<.....<...<<<<.<.....>.>>>>...>..>
>.>>..........<<<.<<.<<.<.<<<<<<<<.<<<........>>>>
>>>>>>>..>>>...<<..]]>>...>>.....>>>.>>>.<<<......
<<<......>>>....>>>..]]]].}.<<<<.<<<<<<<..<<.<<<<<
...<<<.<<<<<<......<<........>>..........<<<<<.<..
..<<<<<........<<..<..........>..>>......>>>>>...<
<.<<<....<<<<<<.....<<<.<<<<......>>...<<<......>>
>...>>.>>>....<<<<..<<..<.<<..<<<<<.<<<<<<....>>>>
>>...>>>>>..>>.>.>>....<<<<<<.....>>>>>>........>>
>>.....>>.>>>...>...>>>>>.....>.>>>>>...>>.>>>>.>>
>.....<<<<<<<......<<.....<<...<<<....>>>...>>....
>>.......>>>>>>>......<....<<<<<<..........>>>>>>.
...>......>>>>>....<<<<<<<<.......>>>>>>>>......>>
...>>>>>>>>>>.>........<<....<<<<.<<....<<<<<<<<.<
<<....<<......<<<<<<..<..<..<.......>...>..>..>>>>
>>......>>....>>>.>>>>>>>>...>>.>>>>.....>>.......
...<<<<<<<<....>>>>>>>>........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5juo Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome.
• Resolution: 4.0 Å
• Binding residue (original residue number in PDB): E53 T57 K391 Q432 R433 P444 X699
• Binding residue (residue number reindexed from 1): E52 T56 K390 Q431 R432 P443 X698

Enzymatic activity

• Enzyme Commision number: 3.6.5.-

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0019843 rRNA binding
• GO:0042802 identical protein binding
• GO:0043022 ribosome binding
• GO:0051087 protein-folding chaperone binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0045901 positive regulation of translational elongation
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5juo, PDBe:5juo, PDBj:5juo
• PDBsum: 5juo
• PubMed: 27159452
• UniProt: P32324|EF2_YEAST Elongation factor 2 (Gene Name=EFT1)