Structure of PDB 8i3o Chain D Binding Site BS01

Receptor Information

>8i3o Chain D (length=152) Species: 559292 (Saccharomyces cerevisiae S288C)

ASKWDQKGMDIAYEEAALGYKEGGVPIGGCLINNKDGSVLGRGHNMRFQK
GSATLHGEISTLENCGRLEGKVYKDTTLYTTLSPCDMCTGAIIMYGIPRC
VVGENVNFKSKGEKYLQTRGHEVVVVDDERCKKIMKQFIDERPQDWFEDI
GE

Ligand information

• Ligand ID: MN
• InChI: InChI=1S/Mn/q+2
• InChIKey: WAEMQWOKJMHJLA-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mn+2]
  CACTVS 3.341: [Mn++]
• Formula: Mn
• Name: MANGANESE (II) ION
• DrugBank: DB06757
• PDB chain: 8i3o Chain D Residue 201

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8i3o crystal structure of yeast cytosine deaminase mutant yCD-RQ-1/8SAH
• Resolution: 2.0 Å
• Binding residue (original residue number in PDB): H62 C91 C94
• Binding residue (residue number reindexed from 1): H56 C85 C88
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 3.5.4.1: cytosine deaminase.

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004131 cytosine deaminase activity
• GO:0008270 zinc ion binding
• GO:0008835 diaminohydroxyphosphoribosylaminopyrimidine deaminase activity
• GO:0016787 hydrolase activity
• GO:0016814 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amidines
• GO:0019239 deaminase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0008655 pyrimidine-containing compound salvage
• GO:0019858 cytosine metabolic process
• GO:0034654 nucleobase-containing compound biosynthetic process
• GO:0044206 UMP salvage
• GO:0046087 cytidine metabolic process

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm

External links

• PDB: RCSB:8i3o, PDBe:8i3o, PDBj:8i3o
• PDBsum: 8i3o
• UniProt: Q12178|FCY1_YEAST Cytosine deaminase (Gene Name=FCY1)