Structure of PDB 8b0x Chain D Binding Site BS01

Receptor Information

>8b0x Chain D (length=205) Species: 37762 (Escherichia coli B)

ARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGARKPRLSDY
GVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNV
VYRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKK
QSRVKAALELAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIV
ELYSK

Ligand information

>8b0x Chain A (length=1500)

aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacaggaauucgcugacgaguggcggacggg
ugaguaaugucugggaaacugccugauggagggggauaacuacuggaaac
gguagcuaauaccgcauaacgucgcaagaccaaagagggggccucuugcc
aucggaugugcccagaugggauuagcuaguaggugggguaacggcucacc
uaggcgacgaucccuagcuggucugagaggaugaccagccacacuggaac
ugagacacgguccagacuccuacgggaggcagcaguggggaauauugcac
aaugggcgcaagccugaugcagccaugccgcguguaugaagaaggccuuc
ggguuguaaaguacuuucagcggggaggaagggaguaaaguuaauaccuu
ugcucauugacguuacccgcagaagaagcaccggcuaacuccgugccagc
agccgcgguaauacggagggugcaagcguuaaucggaauuacugggcgua
aagcgcacgcaggcgguuuguuaagucagaugugaaauccccgggcucaa
ccugggaacugcaucugauacuggcaagcuugagucucguagaggggggu
agaauuccagguguagcggugaaaugcguagagaucuggaggaauaccgg
uggcgaaggcggcccccuggacgaagacugacgcucaggugcgaaagcgu
ggggagcaaacaggauuagauacccugguaguccacgccguaaacgaugu
cgacuuggagguugugccggcguggcuuccggagcuaacgcguuaagucg
accgccuggggaguacggccgcaagguuaaaacucaaaugaauugacggg
ggcccgcacaagcgguggagcaugugguuuaauucgaugcaacgcgaaga
accuuaccuggucuugacauccacggaaguuuucagagaugagaaugugc
gaaccgugagacaggugcugcauggcugucgucagcucguguugugaaau
guuggguuaagucccgcaacgagcgcaacccuuauccuuuguugccagcg
guccggccgggaacucaaaggagacugccagugauaaacuggaggaaggu
ggggaugacgucaagucaucauggcccuuacgaccagggcuacacacgug
cuacaauggcgcauacaaagagaagcgaccucgcgagagcaagcggaccu
cauaaagugcgucguaguccggauuggagucugcaacucgacuccaugaa
gucggaaucgcuaguaaucguggaucagaaugccacggugaauacguucc
cgggccuuguacacaccgcccgucacaccaugggaguggguugcaaaaga
aguagguagcuuaaccuucgggagggcgcuuaccacuuugugauucauga
cuggggugaagucguaacaagguaaccguaggggaaccugcgguuggauc

.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<<..<<<<<..>>.>>>>>..>>>>>......<
<.......<<<<<<<..<<...<<<<<<<.<.<<.....<<<<<......
>>>>>......>>.>.....<<<....>>>....<<<<<<.>>>>>>.>>
>>>>>..>>.>>>>>>><<<....<<<..<<<<<<<.........>>>>>
>>>>>......>>>..<<<<<<<<....>>>>...>>>>.>>.<<<<<.<
.........>>>>>>.<<<<....>>>>...>>>>>>.........<<<.
...<<<<....>>>>..>>>..>>.>>>>>>..<<<<......<<<<...
.>>>>.....>>>>...<.<<<<<......<.<<<<<<<<.......>>>
>>>>>.>........>>>>>....>..<<<<<(((...<<<<<.....<<
.)))>>.......>>>>>>>>>>..>>>>>>>>>..........<<<((.
....<<<<...<<<.<<<<<<<.<<<<<<<<<<......<<<<<<.....
>>>>>>....>>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<
....<<<<<<<<...<<<......>>>......>>>>>>>>.........
..<<....>>.>>>>>>>..>>>>.>>>>...>>>...>>>>....<<<<
<<...<<...<<<<.<.....>.>>>>...>>>>>>>>..........<<
<<<<.<<<<<<<<<<<<..>>>>>>>>>>>>..<<..))>>.....>>>>
>>.>>>.<<<......<<<<....>>>>....>>>..)))).]<<<<<.<
<<<<<<.<<.<<<<<<..<<<<<<<<<<......<<........>>....
......<<<<<<<......<<<<<<<..<<<<<<<....>>>>>>>....
...>>>>>.>>.<<<.<<<..<<<<<<.......<<<<<<<<<....>>>
..<<<<......>>>>..>>>>>>.....<<<<.<<<<<<<...<<..<<
<.....>>>>>....>>>>>>>.....<<<<<.....>>>>>........
>>>>.........>>>...>>>>>>>>>...>>>>>>>...>>.>>>>>>
>>.....<<<<<<<.....<<<..<<...<<<....>>>...>>....>>
>.....>>>>>>>......<....<<<<<<<........>>>>>>>....
>.....>>>>>>....<<<<<<<.........>>>>>>>......>>...
>>>>>>>>>>.>>....<..<<.<.<<<<.<<<..<<<<<<<<<<<<...
.<<<<<<.<<<<..<<....>>.>>>>>>>>>>...>>>>>>>>>>>>..
>>>.>>>>..>.>>...>.....<<<<<<<<<....>>>>>>>>>.....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8b0x The translating bacterial ribosome at 1.55 angstrom resolution generated by cryo-EM imaging services.
• Resolution: 1.55 Å
• Binding residue (original residue number in PDB): A2 R3 L5 P7 K8 L9 K10 R13 R14 K22 S23 R26 K31 C32 K33 G39 Q40 H41 R44 S49 Y51 L55 R56 Q59 R62 L68 E69 R70 Q71 R81 K83 E113 R115 Q116 S119 H120 K121 V130 N131 I132 S134 Y135 R146 Q152 E202 S205 K206
• Binding residue (residue number reindexed from 1): A1 R2 L4 P6 K7 L8 K9 R12 R13 K21 S22 R25 K30 C31 K32 G38 Q39 H40 R43 S48 Y50 L54 R55 Q58 R61 L67 E68 R69 Q70 R80 K82 E112 R114 Q115 S118 H119 K120 V129 N130 I131 S133 Y134 R145 Q151 E201 S204 K205

Gene Ontology

Molecular Function

• GO:0000900 mRNA regulatory element binding translation repressor activity
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0048027 mRNA 5'-UTR binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006353 DNA-templated transcription termination
• GO:0006412 translation
• GO:0006417 regulation of translation
• GO:0031564 transcription antitermination
• GO:0042254 ribosome biogenesis
• GO:0042274 ribosomal small subunit biogenesis
• GO:0045947 negative regulation of translational initiation
• GO:0046677 response to antibiotic
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8b0x, PDBe:8b0x, PDBj:8b0x
• PDBsum: 8b0x
• PubMed: 36841832
• UniProt: P0A7V8|RS4_ECOLI Small ribosomal subunit protein uS4 (Gene Name=rpsD)