Structure of PDB 7mi9 Chain D Binding Site BS01

Receptor Information
>7mi9 Chain D (length=339) Species: 243231 (Geobacter sulfurreducens PCA) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
RGLPLYVQSPKAYVRKDGDCLVIEEERVRVAEARLGETSQVALFGNATLT
TAALHECLRREIPVTWLSYGGWFMGHTVSTGHRNVETRTYQYQRSFDPET
CLNLARRWIVAKIANCRTLLRRNWRGEGDEAKAPPGLLMSLQDDMRHAMR
APSLEVLLGIEGASAGRYFQHFSRMLRGGDGEGMGFDFTTRNRRPPKDPV
NALLSFAYAMLTREWTVALAAVGLDPYRGFYHQPRFGRPALALDMMEPFR
PLIADSTVLMAINNGEIRTGDFVRSAGGCNLTDSARKRFIAGFERRMEQE
VTHPIFKYTISYRRLLEVQARLLTRYLSGEIPAYPNFVT
Ligand information
>7mi9 Chain G (length=80) [Search DNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
aggacaacgttacggacggcacagcctttttgcttcaatgaggccggggc
atcatggccccggaatacggctcttttccg
Receptor-Ligand Complex Structure
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PDB7mi9 Mechanism for Cas4-assisted directional spacer acquisition in CRISPR-Cas.
Resolution3.89 Å
Binding residue
(original residue number in PDB)		S287 Y288 W291 N411 R412 R413 P415 S424 F425 A428 H451 Q452 R454 R457 L462 N499 K506
Binding residue
(residue number reindexed from 1)		S68 Y69 W72 N192 R193 R194 P196 S205 F206 A209 H232 Q233 R235 R238 L243 N280 K287
Enzymatic activity
Enzyme Commision number 3.1.-.-
3.1.12.1: 5' to 3' exodeoxyribonuclease (nucleoside 3'-phosphate-forming).
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0004527 exonuclease activity
GO:0046872 metal ion binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:7mi9, PDBe:7mi9, PDBj:7mi9
PDBsum7mi9
PubMed34588691
UniProtQ74H36|CS4F1_GEOSL CRISPR-associated exonuclease Cas4/endonuclease Cas1 fusion (Gene Name=cas4-cas1)

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