Structure of PDB 7lfu Chain D Binding Site BS01

Receptor Information

>7lfu Chain D (length=306) Species: 694009 (Severe acute respiratory syndrome-related coronavirus)

TIKVFTTVDNTNLHTQLVDMSMTYGQQFGPTYLDGADVTKIKPHVNHEGK
TFFVLPSDDTLRSEAFEYYHTLDESFLGRYMSALNHTKKWKFPQVGGLTS
IKWADNNCYLSSVLLALQQLEVKFNAPALQEAYYRARAGDAANFCALILA
YSNKTVGELGDVRETMTHLLQHANLESAKRVLNVVCKHCGQKTTTLTGVE
AVMYMGTLSYDNLKTGVSIPGRDATQYLVQQESSFVMMSAPPAEYKLQQG
TFLCANEYTGNYQCGHYTHITAKETLYRIDGAHLTKMSEYKGPVTDVFYK
ETSYTT

Ligand information

>7lfu Chain A (length=4) Species: 32630 (synthetic construct)

PAGE

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7lfu A molecular sensor determines the ubiquitin substrate specificity of SARS-CoV-2 papain-like protease.
• Resolution: 2.29 Å
• Binding residue (original residue number in PDB): N110 C112 L163 G164 D165 P249 Y265 Y269 G272 H273 Y274
• Binding residue (residue number reindexed from 1): N106 C108 L159 G160 D161 P242 Y258 Y262 G265 H266 Y267

Enzymatic activity

• Enzyme Commision number: 2.1.1.-
  2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Gene Ontology

Molecular Function

• GO:0008234 cysteine-type peptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:7lfu, PDBe:7lfu, PDBj:7lfu
• PDBsum: 7lfu
• PubMed: 34547223
• UniProt: P0C6X7|R1AB_SARS Replicase polyprotein 1ab (Gene Name=rep)