Structure of PDB 6w6p Chain D Binding Site BS01

Receptor Information

>6w6p Chain D (length=207) Species: 474186 (Enterococcus faecalis OG1RF)

TKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTVETDGYEAIQV
GYQDKREVLSNKPAKGHVAKANTAPKRFIKEFKNVELGEYEVGKEIKVDV
FQAGDVVDVTGTTKGKGFQGAIKRHGQSRGPMSHGSRYHRRPGSMGPVAP
NRVFKNKRLAGRMGGDRVTIQNLEVVKVDVERNVILIKGNIPGAKKSLIT
IKSAVKA

Ligand information

>6w6p Chain A (length=2739)

uuaagugaauaagggcgcacgguggaugccuuggcacuaggagccgauga
aggacgggacuaacaccgauaugcuuuggggagcuguaaguaagcuauga
uccagagauuuccgaaugggggaacccaauaucuuuuauaggauauuacu
uuucagugaauacauagcugauuagagguagacgcagagaacugaaacau
cuuaguaccugcaggaagagaaagaaaauucgauucccugaguagcggcg
agcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguuguag
gacuccgauaugguagucuguuaguauaguugaaggauuuggaaaauucc
gcuaaagagggugaaagccccguagacgaaaugcugacaacaccuaggag
gauccugaguacggcggaacacgagaaauuccgucggaauccgcggggac
caucccgcaaggcuaaauacucccuagugaccgauagugaaccaguaccg
ugagggaaaggugaaaagcaccccggaaggggagugaaauagauccugaa
accgugugccuacaacaagucaaagcucguuaaugagugauggcgugccu
uuuguagaaugaaccggcgaguuacgauugcaugcgagguuaagucgaag
agacggagccgcagcgaaagcgagucugaauagggcgaaugaguauguag
ucguagacccgaaaccaugugaucuacccauguccagguugaaggugcgg
uaaaacgcacuggaggaccgaacccacguacguugaaaagugcggggaug
agguguggguagcggagaaauuccaaacgaacuuggagauagcugguucu
cuccgaaauagcuuuagggcuagccucggaauugagaaugauggagguag
agcacuguuuggacuaguucagauaaacuccgaaugccauucauuuauau
ccgggagucagacugcgagugauaagauccguagucgaaagggaaacagc
ccagaccaccagcuaaggucccaaaauauauguuaaguggaaaaggaugu
gggguugcacagacaacuaucgagugacccugcgccgaaaauguaccggg
gcuaaacauauuaccgaagcuguggacuacaccauuagguguagugguag
gagagcguucuaagggcguugaaggucgaucgugaggacggcuggagcgc
uuagaagugagaaugccgguaugaguagcgaaagacaggugagaauccug
uccaccguaugacuaagguuuccuggggaaggcucguccgcccaggguua
gucgggaccuaagccgaggccgauaggcguaggcgauggacaacagguug
auauuccuguaccaguuguuuguuugagcaauggagggacgcaguaggcu
aaggaaugcaugcgauuggaagugcauguccaagcaaugagucuugagua
gaguuaaaugcuuuacucuuuaaggacaaguugugacggggagcgaaaua
guagcgaaguuccugacgucacacugccaagaaaagcuucuagugagaaa
acaacugcccguaccguaaaccgacacagguagucgaggagaguauccua
aggugagcgagcgaacucucguuaaggaacucggcaaaaugaccccguaa
cuucgggagaaggggugcugacuucggucagccgcagugaauaggcccaa
gcgacuguuuaucaaaaacacaggucucugcaaaaucguaagaugaagua
uaggggcugacgccugcccggugcuggaagguuaagaggauggguuagcu
ucggcgaagcucagaauugaagccccaguaaacggcggccguaacuauaa
cgguccuaagguagcgaaauuccuugucggguaaguuccgacccgcacga
aaggcguaacgauuugggcacugucucaacgagagacucggugaaauuuu
aguaccugugaagaugcagguuacccgcgacaggacggaaagaccccaug
gagcuuuacuguaguuugauauugaguguuuaacccucuaacccgcacca
cuaaucguggugggagacagugucagaugggcaguuugacuggggcgguc
gccuccuaaaagguaacggaggcgcccaaagguucccucagaaugguugg
aaaucauucgaagaguguaaaggcagaagggagcuugacugcgagaccua
caagucgagcagggacgaaagucgggcuuagugauccggugguuccgcau
ggaagggccaucgcucaacggauaaaagcuacccuggggauaacaggcuu
aucucccccaagaguccacaucgacggggagguuuggcaccucgaugucg
gcucgucgcauccuggggcuguagucggucccaaggguugggcuguucgc
ccauuaaagcggcacgcgagcuggguucagaacgucgugagacaguucgg
ucccuauccgucgcgggcguuggaaauuugagaggagcuguccuuaguac
gagaggaccgggauggacuuaccgcugguguaccaguuguucugccaagg
gcauugcuggguagcuauguagggaagggauaaacgcugaaagcaucuaa
gugugaagcccaccucaagaugagauuucccauuucuuuaagaaaguaag
accccugagagaugaucagguagauagguuggaaguggaaggcuagugau
aguuggagcggaccaauacuaaucggucgaggacuuaac

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
..<<<<<.........>>>>>..>>.>>....<<<<<......<<....>
>.......>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<
<<<<<<...<.<<<.<<.<<<<<<<<<.<<<...<<<.<......>.>>>
((......<<<.....))>>>...>>>...>>>>>>>>>>>>>>>.>>>>
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..)))......]].>>>>>>>.]]...............<<.
...>>....]].........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>>...<.<
........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...<<<....
.>>>...>>>...<<....>>..<<<<.....>>>>......>>>>>>>>
>>>>>..<<.<..<<<<.<<.....<<<<<<<<<..<<<....<<<<<<.
.....>>>>>>.....>>>...<<<.<<<<<........>>>>>>>>...
.>>>>>>>>>...<<<.....>>>......>>.>>>...>..>.>>..<<
<<<<....<<<<<....>>>>><<<<<<<<..<..<<<<<<<<<.....<
<<...<<<<<<<<....>>>>>>>>..>>.>.....>>>>.>>>>>..>>
>>>>>.>>..<<<<<<<.<<......>>.>>>>>>>....<<<......>
>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..<<
<<<<<<<........<...>....>>>>>>>>>.>>...........)))
.....>>>>>>.>>>...>>>>>>><<<<<<<......>>>>>>>....>
>>>>><.<<<<<<<.<<......<<<<<.<<....>>.>>>>>...>>.>
>>>>>>..>.....>>>>>><......<<....<<<<<<.......>>>>
>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>>>....
((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<<<<...
.....>>>>>))<<<<<<<.........<...<<<<....<<<<..<<<.
.<<<<<...........{...........<<..<<<<<..<<<<.<<<<<
<<<<.....>>>>>>>>>.....>>>>..>>>>>...>>...<<......
...>>....>>>>>...>>>...>>>>.........>>>>..>.......
>>>>>>>>>>>..>>.....<<......>>>>>>>.<<<<.....>>>>.
.>>>>..<<<<....<<<<<<<<.<<.........<.....<<<<<....
<<..>>.....>>>>><<<<<<....>>>>>>............<<<<<<
<<<..<<<.........>>>..<<<<<<<.....<<<....>>>......
.>>>>>>>.<<<<<.<<<..<<<<<<<..<<<<..<<...<<<<<..<<.
...>>...>>>>>...>>..>>>>>>>>>..>>>>><<<<<<.......>
>>>>>........<...........<<<<<<.....>>>>>>.....>..
..>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>..
...<<<<<........>>>>>..<<<<<<<<.(((.[[......<<<<.[
[<<<<..<<<<<.<<<<<<<<<.<<<.<<<.>>>.>>>....<<.<<<<<
<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<.....>>>>
<<<<<<...........>>>>>>..<<...<<<<<<<...<<<<<<....
...>>>>>>.....<<.[.(((>>....>>>>>>>...<<<<..<<<...
...>>>..>>>>.<<<....>>>.>>)))]......<<<<<<<.((..>>
>>..))>>>>>>>.............>>>>..]]>>>>.......<<<<.
<.<<<<<<...<<.......>>...>>>>>>...>>>>.>.......<<<
<<.<<<<<<..<<<<<<<<<......>>>>>>..>>>.<<<<<.....>>
>>>.....>>>>>..>...>>>.>>....<<<<<<<....>>>.>>>>..
..]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<<<<......
.........>>>>>>>.<<<<<...<<<<...<<<<<<<<<<<.}..>>>
>>>..>>>>>...>>>>.>>>>>...<<<.<..<<<<<...........>
>>>>...>>>>.>>>>.......>>>>>>>..<<<......>>>.....<
<<<<<<<<.......>>>>>....<<<<<<<<..<<.....<<<<....>
>>>....>>...>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6w6p Cryo-electron microscopy structure of the 70S ribosome from Enterococcus faecalis.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): M12 Q14 P24 Q37 D44 Y46 R57 V59 N62 K63 P64 G67 H68 R78 E82 T114 K115 G116 K117 G118 F119 G121 K124 R125 Q128 S129 R130 S134 H135 G136 Y139 H140 R141 S145 M146 P148 V149 A150 P151 N152 R153 V154 F155 K158 L160 R163 R168 T170 Q172 N173 N191 I192 P193 K207
• Binding residue (residue number reindexed from 1): M11 Q13 P23 Q36 D43 Y45 R56 V58 N61 K62 P63 G66 H67 R77 E81 T113 K114 G115 K116 G117 F118 G120 K123 R124 Q127 S128 R129 S133 H134 G135 Y138 H139 R140 S144 M145 P147 V148 A149 P150 N151 R152 V153 F154 K157 L159 R162 R167 T169 Q171 N172 N190 I191 P192 K206

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6w6p, PDBe:6w6p, PDBj:6w6p
• PDBsum: 6w6p
• PubMed: 33004869
• UniProt: A0A1B4XKR3