Structure of PDB 5njt Chain D Binding Site BS01

Receptor Information

>5njt Chain D (length=199) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

ARYTGPSWKLSRRLGISLSGTGKELEKRPYAPGPHGPGQRKKLSEYGLQL
QEKQKLRHMYGVNERQFRTLFDKAGKLAGKHGENFMILLDSRLDNVVYKL
GLARTRRQARQLVNHGHILVDGSRVDIPSYLVKPGQTIGVREKSRNLSII
KESVEVNNFVPEYLTFDAEKLEGTFTRLPERSELAPEINEALIVEFYSR

Ligand information

>5njt Chain A (length=1544)

ggagaguuugauccuggcucaggacgaacgcuggcggcgugccuaauaca
ugcaagucgagcggacagaugggagcuugcucccugauguuagcggcgga
cgggugaguaacacguggguaaccugccuguaagacugggauaacuccgg
gaaaccggggcuaauaccggaugguuguuugaaccgcaugguucaaacau
aaaagguggcuucggcuaccacuuacagauggacccgcggcgcauuagcu
aguuggugagguaacggcucaccaaggcgacgaugcguagccgaccugag
agggugaucggccacacugggacugagacacggcccagacuccuacggga
ggcagcaguagggaaucuuccgcaauggacgaaagucugacggagcaacg
ccgcgugagugaugaagguuuucggaucguaaagcucuguuguuagggaa
gaacaagugccguucgaauagggcgguaccuugacgguaccuaaccagaa
agccacggcuaacuacgugccagcagccgcgguaauacguagguggcaag
cguuguccggaauuauugggcguaaagggcucgcaggcgguuucuuaagu
cugaugugaaagcccccggcucaaccggggagggucauuggaaacugggg
aacuugagugcagaagaggagaguggaauuccacguguagcggugaaaug
cguagagauguggaggaacaccaguggcgaaggcgacucucuggucugua
acugacgcugaggagcgaaagcguggggagcgaacaggauuagauacccu
gguaguccacgccguaaacgaugagugcuaaguguuaggggguuuccgcc
ccuuagugcugcagcuaacgcauuaagcacuccgccuggggaguacgguc
gcaagacugaaacucaaaggaauugacgggggcccgcacaagcgguggag
caugugguuuaauucgaagcaacgcgaagaaccuuaccaggucuugacau
ccucugacaauccuagagauaggacguccccuucgggggcagagugacag
guggugcaugguugucgucagcucgugucgugagauguuggguuaagucc
cgcaacgagcgcaacccuugaucuuaguugccagcauucaguugggcacu
cuaaggugacugccggugacaaaccggaggaagguggggaugacgucaaa
ucaucaugccccuuaugaccugggcuacacacgugcuacaauggacagaa
caaagggcagcgaaaccgcgagguuaagccaaucccacaaaucuguucuc
aguucggaucgcagucugcaacucgacugcgugaagcuggaaucgcuagu
aaucgcggaucagcaugccgcggugaauacguucccgggccuuguacaca
ccgcccgucacaccacgagaguuuguaacacccgaagucggugagguaac
cuuuuaggagccagccgccgaaggugggacagaugauuggggugaagucg
uaacaagguagccguaucggaaggugcggcuggaucaccuccuu

...<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....<<<.
<<<..<<<..<<.<<<<...<<<<<....>>>>>...>>>>.>>>>>...
...<<........<<<<<<<..<<...<<<<<<<.<<<<.....<<<<<<
....>>>>>>......>>>>.....<<<<<<<<<<....>>>>>>>>>>.
....<<<<<<....>>>>>>.>>>>>>>..>>>>>>>>>.<<<....<<<
..<<<<<<<<.......>>>>>>>>>>>......>>>..<<<<<<<<...
.>>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>
>...>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>>
>>>>..<<<<......<<<<....>>>>.....>>>>..<<<<<<<<...
....<<<<<<<<<<<.....>>>>>>>>>.>>.......>>>>>>...>>
.<<<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>
>>>>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<
<<<<<<.......<<<<<<.....>>>>>>.....>>>>>>>..>>>>>>
>>>...<<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>
>......>>>>>>>>...........<<....>>.>>>>>>>..>>>>>.
>>>...>>>...>>>>....<<<<<<...<<...<<<<.<.....>.>>>
>...>>>>>>>>..........<<<<<<..<<<<<<<<<<<<......>>
>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<......<<<<
....>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<
<<<<<<<<......<<........>>..........<<<<<<<......<
<<<<<<....<<<<<....>>>>>....<<......>>.>>>>>.>>.<<
<.<<<..<<<<<<.......<<<<<<<<<....>>>..<<<<......>>
>>..>>>>>>.....<<<..<<<<<<<..<<<..<<......>>>>>...
>>>>>>>.....<<<<<.....>>>>>.........>>>.........>>
>...>>>>>>>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.
....<<<..<<..<<<<....>>>>..>>....>>>.....>>>>>>>..
....<....<<<<<<<........>>>>>>>....>.....>>>>>>...
.<<<<<<<.........>>>>>>>......>>...>>>>>>>>>>.>>..
..<..<<.<.<<<<.<<<..<<<<<<..<<<<....<.<<<<..<<<..<
<<...>>>.>>>.>>>>.>...>>>>..>>>>>>..>>>.>>>>..>.>>
...>.....<<<<<<<<<....>>>>>>>>>.............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5njt Structure of the Bacillus subtilis hibernating 100S ribosome reveals the basis for 70S dimerization.
• Resolution: 3.8 Å
• Binding residue (original residue number in PDB): A2 P7 S8 W9 G34 P35 N64 E65 D127 E196 S199 R200
• Binding residue (residue number reindexed from 1): A1 P6 S7 W8 G33 P34 N63 E64 D126 E195 S198 R199

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5njt, PDBe:5njt, PDBj:5njt
• PDBsum: 5njt
• PubMed: 28468753
• UniProt: P21466|RS4_BACSU Small ribosomal subunit protein uS4 (Gene Name=rpsD)