Structure of PDB 3zh2 Chain D Binding Site BS01

Receptor Information

>3zh2 Chain D (length=311) Species: 36329 (Plasmodium falciparum 3D7)

PKAKIVLVGSGMIGGVMATLIVQKNLGDVVLFDIVKNMPHGKALDTSHTN
VMAYSNCKVSGSNTYDDLAGADVVIVTAGFTKAPDKEWNRDDLLPLNNKI
MIEIGGHIKKNCPNAFIIVVTNPVDVMVQLLHQHSGVPKNKIIGLGGVLD
TSRLKYYISQKLNVCPRDVNAHIVGAHGNKMVLLKRYITVGGIPLQEFIN
NKLISDAELEAIFDRTVNTALEIVNLHASPYVAPAAAIIEMAESYLKDLK
KVLICSTLLEGQYGHSDIFGGTPVVLGANGVEQVIELQLNSEEKAKFDEA
IAETKRMKALA

Ligand information

>3zh2 Chain G (length=27)

ctgggcggtagaaccatagtgacccag

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3zh2 Structural Basis for Discriminatory Recognition of Plasmodium Lactate Dehydrogenase by a DNA Aptamer
• Resolution: 2.1 Å
• Binding residue (original residue number in PDB): G13 I36 V37 M40 K44 A80 F82 K84 I105
• Binding residue (residue number reindexed from 1): G11 I34 V35 M38 K42 A78 F80 K82 I100
• Binding affinity: PDBbind-CN: Kd=42nM

Enzymatic activity

• Catalytic site (original residue number in PDB): R95 D155 R158 H182
• Catalytic site (residue number reindexed from 1): R90 D150 R153 H177
• Enzyme Commision number: 1.1.1.27: L-lactate dehydrogenase.

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004459 L-lactate dehydrogenase activity
• GO:0005515 protein binding
• GO:0016491 oxidoreductase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0030060 L-malate dehydrogenase (NAD+) activity

Biological Process

• GO:0006096 glycolytic process
• GO:0019752 carboxylic acid metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3zh2, PDBe:3zh2, PDBj:3zh2
• PDBsum: 3zh2
• PubMed: 24043813
• UniProt: Q76NM3