Structure of PDB 3wfs Chain D Binding Site BS01

Receptor Information

>3wfs Chain D (length=445) Species: 32630 (synthetic construct)

AAALNFYLSYFDDVAKVLPREHYCFIVGGWVRDRILGEPVGYNIDVDFLT
TADPVELAKNFAKRIGGHFFVFEKRGFLIKRPTIASVVLHLPPYRYRFDF
SPLKGKDLEKALIEDLKERDFTANAIAVNLDDVLTIVYDPTGGIKDLEQG
LLRPVSIENLKRDPVRVLRGFRIAIEKNLQLTEDFYEFVKEDPRIVLKSA
VERITHELFKIMKEKTAHKVIRELYEYGVLEAIIPEIGRLREVKDPLDEH
TLKTLEYLEQVIEDRAKYLSAELLENFGKKRVLGEFTDVELLKWGALFHD
IGKPQTFAFYEHDKVGAQIVREIGERLRWGDEATEFVAKLVRHHLRPFFL
REAFKKGELKRRGMANFWRECGDIAPHLFLLSIADAMASGDEEEDIKALM
ETIAELESFNRNEMKAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA

Ligand information

>3wfs Chain B (length=74)

ggccagguagcucaguugguagagcacuggacugaaaauccaggugucgg
cgguucgauuccgccccuggccac

<<<<<<<..<<<<........>>>>.<<<<.........>>>>.....<<
<<<.......>>>>>>>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3wfs Translocation and rotation of tRNA during template-independent RNA polymerization by tRNA nucleotidyltransferase
• Resolution: 3.311 Å
• Binding residue (original residue number in PDB): D58 D60 F83 F85 K87 R88 R94 D112 R132 D133 N137 R185 R188 E221 R222 D364 X473
• Binding residue (residue number reindexed from 1): D45 D47 F70 F72 K74 R75 R81 D99 R119 D120 N124 R166 R169 E202 R203 D331 X416

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0016779 nucleotidyltransferase activity

Biological Process

• GO:0006396 RNA processing

External links

• PDB: RCSB:3wfs, PDBe:3wfs, PDBj:3wfs
• PDBsum: 3wfs
• PubMed: 24389024
• UniProt: O67911|CATNT_AQUAE CC-adding tRNA nucleotidyltransferase (Gene Name=aq_2158)