Structure of PDB 3siu Chain D Binding Site BS01

Receptor Information
>3siu Chain D (length=125) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
ADVNPKAYPLADAHLTKKLLDLVQQSCNYKQLRKGANEATKTLNRGISEF
IVMAADAEPLEIILHLPLLCEDKNVPYVFVRSKQALGRACGVSRPVIACS
VTIKEGSQLKQQIQSIQQSIERLLV
Ligand information
>3siu Chain F (length=28) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cuguccaaugagcgcauagugagggcag
<<<<<<.....<.......>..>>>>>>
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB3siu Structural basis for the dual U4 and U4atac snRNA-binding specificity of spliceosomal protein hPrp31.
Resolution2.626 Å
Binding residue
(original residue number in PDB)		K37 G38 A39 N40 E41 K44 R48 A60 E61 I65 V95 R97 V99 I100
Binding residue
(residue number reindexed from 1)		K34 G35 A36 N37 E38 K41 R45 A57 E58 I62 V92 R94 V96 I97
Binding affinityPDBbind-CN: Kd=8uM
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0030515 snoRNA binding
GO:0030621 U4 snRNA binding
GO:0030622 U4atac snRNA binding
GO:0034511 U3 snoRNA binding
GO:0034512 box C/D sno(s)RNA binding
GO:0051117 ATPase binding
Biological Process
GO:0000398 mRNA splicing, via spliceosome
GO:0000492 box C/D snoRNP assembly
GO:0006397 mRNA processing
GO:0007338 single fertilization
GO:0008380 RNA splicing
GO:0030490 maturation of SSU-rRNA
GO:0042254 ribosome biogenesis
GO:0042274 ribosomal small subunit biogenesis
Cellular Component
GO:0001651 dense fibrillar component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0005690 U4atac snRNP
GO:0005730 nucleolus
GO:0031428 box C/D methylation guide snoRNP complex
GO:0032040 small-subunit processome
GO:0032991 protein-containing complex
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071005 U2-type precatalytic spliceosome
GO:0071011 precatalytic spliceosome
GO:1990904 ribonucleoprotein complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3siu, PDBe:3siu, PDBj:3siu
PDBsum3siu
PubMed21784869
UniProtP55769|NH2L1_HUMAN NHP2-like protein 1 (Gene Name=SNU13)

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