Structure of PDB 3cwt Chain D Binding Site BS01

Receptor Information

>3cwt Chain D (length=281) Species: 83333 (Escherichia coli K-12)

MYTLNWQPPYDWSWMLGFLAARAVSSVETVADSYYARSLAVGEYRGVVTA
IPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGRLGA
ARPGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFP
EYICFPTPQRLAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPG
DVEQAMKTLQTFPGIGRWTANYFALRGWQAKDVFLPDDYLIKQRFPGMTP
AQIRRYAERWKPWRSYALLHIWYTEGWQPDE

Ligand information

>3cwt Chain G (length=12)

gacatgagtgcc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3cwt Structure of the E. coli DNA Glycosylase AlkA Bound to the Ends of Duplex DNA: A System for the Structure Determination of Lesion-Containing DNA.
• Resolution: 2.3 Å
• Binding residue (original residue number in PDB): Q124 L125 G214 I215 G216 T219
• Binding residue (residue number reindexed from 1): Q124 L125 G214 I215 G216 T219

Enzymatic activity

• Catalytic site (original residue number in PDB): W218 Y222 D238
• Catalytic site (residue number reindexed from 1): W218 Y222 D238
• Enzyme Commision number: 3.2.2.21: DNA-3-methyladenine glycosylase II.

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003905 alkylbase DNA N-glycosylase activity
• GO:0005515 protein binding
• GO:0008725 DNA-3-methyladenine glycosylase activity
• GO:0016787 hydrolase activity
• GO:0032131 alkylated DNA binding
• GO:0043916 DNA-7-methylguanine glycosylase activity
• GO:0052821 DNA-7-methyladenine glycosylase activity
• GO:0052822 DNA-3-methylguanine glycosylase activity

Biological Process

• GO:0006281 DNA repair
• GO:0006284 base-excision repair
• GO:0006285 base-excision repair, AP site formation
• GO:0006307 DNA alkylation repair
• GO:0006950 response to stress
• GO:0006974 DNA damage response

Cellular Component

• GO:0005737 cytoplasm
• GO:0032993 protein-DNA complex

External links

• PDB: RCSB:3cwt, PDBe:3cwt, PDBj:3cwt
• PDBsum: 3cwt
• PubMed: 18682218
• UniProt: P04395|3MG2_ECOLI DNA-3-methyladenine glycosylase 2 (Gene Name=alkA)