Structure of PDB 3b0v Chain D Binding Site BS01

Receptor Information
>3b0v Chain D (length=315) Species: 300852 (Thermus thermophilus HB8) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MLDPRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERL
LAFRPEEHPIALQLAGSDPKSLAEAARIGEAFGYDEINLNLGCPSEKAQE
GGYGACLLLDLARVREILKAMGEAVRVPVTVKMRLGLEGKETYRGLAQSV
EAMAEAGVKVFVVHARSALLALSTKANREIPPLRHDWVHRLKGDFPQLTF
VTNGGIRSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADRRVFGLPRRPS
RLEVARRMRAYLEEEVLKGTPPWAVLRHMLNLFRGRPKGRLWRRLLSEGR
SLQALDRALRLMEEE
Ligand information
>3b0v Chain B (length=73) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gccgagguagcucaguugguagagcaugcgacugaaaaucgcagugucgg
cgguucgauuccgccccucggca
<<<<.<<..<<<<........>>>>.<<<<<.......>>>>>.....<<
<<<.......>>>>>>>.>>>>.
Receptor-Ligand Complex Structure
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PDB3b0v Molecular basis of dihydrouridine formation on tRNA
Resolution3.51 Å
Binding residue
(original residue number in PDB)		V12 R14 M36 V38 Q40 N46 C93 S95 K97 E100 G101 Y103 T174 R178 R284 G285 R286 K288 G289 R290 R293 R294 S297 R300
Binding residue
(residue number reindexed from 1)		V12 R14 M36 V38 Q40 N46 C93 S95 K97 E100 G101 Y103 T174 R178 R284 G285 R286 K288 G289 R290 R293 R294 S297 R300
Enzymatic activity
Enzyme Commision number 1.3.1.-
1.3.1.91: tRNA-dihydrouridine(20) synthase [NAD(P)(+)].
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0010181 FMN binding
GO:0016491 oxidoreductase activity
GO:0017150 tRNA dihydrouridine synthase activity
GO:0050660 flavin adenine dinucleotide binding
GO:0102264 tRNA-dihydrouridine20 synthase activity
GO:0102266 tRNA-dihydrouridine20a synthase activity
Biological Process
GO:0002943 tRNA dihydrouridine synthesis
GO:0008033 tRNA processing

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Molecular Function
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Biological Process
External links
PDB RCSB:3b0v, PDBe:3b0v, PDBj:3b0v
PDBsum3b0v
PubMed22123979
UniProtQ5SMC7|DUSAL_THET8 tRNA-dihydrouridine(20/20a) synthase (Gene Name=dus)

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