Structure of PDB 3akz Chain D Binding Site BS01

Receptor Information
>3akz Chain D (length=463) Species: 243274 (Thermotoga maritima MSB8) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
LVRVRFAPSPTGHLHVGGARTALFNWMFARKEGGKFILRIEDTDTERSSR
EYEQQILESLRWCGLDWDEGPDIGGDFGPYRQSERLEIYREYAEKLVEDK
RAYYVVYDKEDPSKELFTTYEYPHEYKEKGHPVTIKFKVLPGKTSFEDLL
KGYMEFDNSTLEDFIIMKSNGFPTYNFAVVVDDHLMRISHVFRGEDHLSN
TPKQLMIYEAFGWEAPVFMHIPLILGSDRTPLSKRHGATSVEHFRREGIL
SRALMNYLALLGWRVEGDEIFTIEEKLQSFDPKDISNKGVIFDYQKLEWV
NGKHMRRIDLEDLKREFIEWAKYAGKEIPSVDERYFSETLRICREKVNTL
SQLYDIMYPFMNDDYEYEKDYVEKFLKREEAERVLEEAKKAFKDLNSWNM
EEIEKTLRDLSEKGLASKKVVFQLIRGAVTGKLVTPGLFETIEVLGKERT
LKRLERTLQFLKK
Ligand information
>3akz Chain H (length=74) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
ugggaggucgucuaacgguaggacggcggacucuggauccgcugguggag
guucgaguccuccccucccagcca
<<<<<<<..<<<<.......>>>><<<<<<.......>>>>>>...<<<<
<.......>>>>>>>>>>>>....
Receptor-Ligand Complex Structure
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PDB3akz Two enzymes bound to one transfer RNA assume alternative conformations for consecutive reactions.
Resolution2.9 Å
Binding residue
(original residue number in PDB)		T66 D67 E69 Y130 K174 M177 F179 T183 I188 T197 Y198 D219 L221 S222 N223 K226 R252 P254 G285 R287 S309 N310 K311 V313 I314 Q318 K319 W322 K326 R329 E368 K369 V370 N371 R449 L456 V457 T458 P459 L461 F462
Binding residue
(residue number reindexed from 1)		T43 D44 E46 Y107 K151 M154 F156 T160 I165 T174 Y175 D196 L198 S199 N200 K203 R229 P231 G262 R264 S286 N287 K288 V290 I291 Q295 K296 W299 K303 R306 E345 K346 V347 N348 R426 L433 V434 T435 P436 L438 F439
Enzymatic activity
Catalytic site (original residue number in PDB) S32 K257
Catalytic site (residue number reindexed from 1) S9 K234
Enzyme Commision number 6.1.1.17: glutamate--tRNA ligase.
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004818 glutamate-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006424 glutamyl-tRNA aminoacylation
GO:0043039 tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:3akz, PDBe:3akz, PDBj:3akz
PDBsum3akz
PubMed20882017
UniProtQ9X2I8|SYE2_THEMA Glutamate--tRNA ligase 2 (Gene Name=gltX2)

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