Structure of PDB 2jg3 Chain D Binding Site BS01

Receptor Information

>2jg3 Chain D (length=391) Species: 271 (Thermus aquaticus)

TPPEVVDFMVSLAEAPRGGRVLEPACAHGPFLRAFREAHGTAYRFVGVEI
DPKALDLPPWAEGILADFLLWEPGEAFDLILGNPPYGIVGEASKYPIHVF
KAVKDLYKKAFSTWKGKYNLYGAFLEKAVRLLKPGGVLVFVVPATWLVLE
DFALLREFLAREGKTSVYYLGEVFPQKKVSAVVIRFQKSGKGLSLWDTQE
SESGFTPILWAEYPHWEGEIIRFETEETRKLEISGMPLGDLFHIRFAARS
PEFKKHPAVRKEPGPGLVPVLTGRNLKPGWVDYEKNHSGLWMPKERAKEL
RDFYATPHLVVAHTKGTRVVAAWDERAYPWREEFHLLPKEGVRLDPSSLV
QWLNSEAMQKHVRTLYRDFVPHLTLRMLERLPVRREYGFHT

Ligand information

>2jg3 Chain E (length=10)

gttcgatgtc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2jg3 Quantitative Labeling of Long Plasmid DNA with Nanometer Precision.
• Resolution: 1.9 Å
• Binding residue (original residue number in PDB): Y108 K116 K139 T167 L171 E172 D173 K199 K200 F268 R271 H394 T413
• Binding residue (residue number reindexed from 1): Y86 K94 K117 T145 L149 E150 D151 K177 K178 F246 R249 H372 T391

Enzymatic activity

• Catalytic site (original residue number in PDB): N105 P106 Y108 F196
• Catalytic site (residue number reindexed from 1): N83 P84 Y86 F174
• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:2jg3, PDBe:2jg3, PDBj:2jg3
• PDBsum: 2jg3
• PubMed: 17654629
• UniProt: P14385|MTTA_THEAQ Type II methyltransferase M.TaqI (Gene Name=taqIM)