Structure of PDB 2i82 Chain D Binding Site BS01

Receptor Information
>2i82 Chain D (length=216) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
ENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKDSVMTRIQRD
YPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWGH
PSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVV
LKPITGRSHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTIT
HPAYGNSMTFKAPADF
Ligand information
>2i82 Chain H (length=21) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gaggggauugaaaauccccuc
<<<<<<.........>>>>>>
Receptor-Ligand Complex Structure
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PDB2i82 Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure
Resolution2.05 Å
Binding residue
(original residue number in PDB)		G937 R938 H961 R962 D964 M965 K977 E980 R981 K984 F987 R988 R990 K994 P1023 Q1025 K1033 G1059 R1060 S1061 H1062 R1079 F1080
Binding residue
(residue number reindexed from 1)		G34 R35 H58 R59 D61 M62 K74 E77 R78 K81 F84 R85 R87 K91 P120 Q122 K130 G156 R157 S158 H159 R176 F177
Enzymatic activity
Enzyme Commision number 5.4.99.28: tRNA pseudouridine(32) synthase.
5.4.99.29: 23S rRNA pseudouridine(746) synthase.
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0009982 pseudouridine synthase activity
GO:0016853 isomerase activity
GO:0106029 tRNA pseudouridine synthase activity
GO:0120159 rRNA pseudouridine synthase activity
GO:0140098 catalytic activity, acting on RNA
GO:0160142 23S rRNA pseudouridine(746) synthase activity
GO:0160151 tRNA pseudouridine(32) synthase activity
Biological Process
GO:0000455 enzyme-directed rRNA pseudouridine synthesis
GO:0001522 pseudouridine synthesis
GO:0006364 rRNA processing
GO:0006396 RNA processing
GO:0008033 tRNA processing
GO:0009451 RNA modification
GO:0031118 rRNA pseudouridine synthesis
GO:0031119 tRNA pseudouridine synthesis

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Molecular Function
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Biological Process
External links
PDB RCSB:2i82, PDBe:2i82, PDBj:2i82
PDBsum2i82
PubMed17188032
UniProtP0AA37|RLUA_ECOLI Dual-specificity RNA pseudouridine synthase RluA (Gene Name=rluA)

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