Structure of PDB 4v95 Chain CS Binding Site BS01

Receptor Information

>4v95 Chain CS (length=75) Species: 300852 (Thermus thermophilus HB8)

FVDDHLLEKVLELNAKGEKRLIKTWSRRSTIVPEMVGHTIAVYNGKQHVP
VYITENMVGHKLGEFAPTRTYRGHG

Ligand information

>4v95 Chain CA (length=1461)

uuggagaguuugauccuggcucagggugaacgcuggcggcgugccuaaga
caugcaagucgugcgggccgcguggucagcggcggacgggugaguaacgc
gugggugaccuacccggaagagggggacaacccggggaaacucgggcuaa
ucccccauguggacccgccccuugggguguguccaaagggcuuugcccgc
uuccggaugggcccgcgucccaucagcuaguuggugggguaauggcccac
caaggcgacgacggguagccggucugagaggauggccggccacaggggca
cugagacacgggccccacuccuacgggaggcagcaguuaggaaucuuccg
caaugggcgcaagccugacggagcgacgccgcuuggaggaagaagcccuu
cgggguguaaacuccugaacccgggacgaaacccccgacgaggggacuga
cgguaccgggguaauagcgccggccaacuccgugccagcagccgcgguaa
uacggagggcgcgagcguuacccggauucacugggcguaaagggcgugua
ggcggccuggggcgucccaugugaaagaccacggcucaaccgugggggag
cgugggauacgcucaggcuagacggugggagagggugguggaauucccgg
aguagcggugaaaugcgcagauaccgggaggaacgccgauggcgaaggca
gccaccugguccacccgugacgcugaggcgcgaaagcguggggagcaaac
cggauuagauacccggguaguccacgcccuaaacgaugcgcgcuaggucu
cugggucuccugggggccgaagcuaacgcguuaagcgcgccgccugggga
guacggccgcaaggcugaaacucaaaggaauugacgggggcccgcacaag
cgguggagcaugugguuuaauucgaagcaacgcgaagaaccuuaccaggc
cuugacaugcccgggugaaagccugggcacaggugcugcauggccgucgu
cagcucgugccgugagguguuggguuaagucccgcaacgagcgcaacccc
cgccguuaguugccagcgguucggccgggcacucuaacgggacugcccgc
gaaagcgggaggaaggaggggacgacgucuggucagcauggcccuuacgg
ccugggcgacacacgugcuacaaugcccacuacaaagcgaugccacccgg
caacggggagcuaaucgcaaaaaggugggcccaguucggauuggggucug
caacccgaccccaugaagccggaaucgcuaguaaucgcggaucagccaug
ccgcggugaauacguucccgggccuuguacacaccgcccgucacgccaug
ggagcgggcucuacccgaagucgccgggagccuacgggcaggcgccgagg
guagggcccgugacuggggcgaagucguaacaagguagcuguaccggaag
gugcggcugga

.....<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<..<..<<
<.<<<..<<<..<<.<<<<<..>>>>>.>>>>>......<<.......<<
<<<<<<..<<...<<<<<<<.<<<<<....<<<<<......>>>>>....
.>>>>>...<<<<<.<<<<<....>>>>>.>>>>>..<<<<...>>>>.>
>>>>>>..>>>>>>>>>><<<....<<<..<<<<<<<<.......>>>>>
>>>>>>......>>>..<<<<<<<<....>>>>...>>>>.>>.<<<<<.
<.........>>>>>>.<<<<....>>>>...>>>>>>>........<<<
....<<<<....>>>>..>>>..>>.>>>>>>..<<<<......<<<<..
..>>>>.....>>>>....<<<<<......<.<<<<.....>>>>..>..
.....>>>>>......<<<<<(((...<<<<<.....<<.)))>>.....
..>>>>>>>>>>..>>>>>>>>>..........<<<((.....<<<<...
<<<.<<<<<<<.<<<<<<<<<<......<<<<<<.....>>>>>>....>
>>>>>>>..>>>>>>>>>...<<<<<<<<...<<<<<<<....<<<<<<<
.<..<<<......>>>.....>.>>>>>>>...........<<....>>.
>>>>>>>..>>>>>.>>>...>>>...>>>>....<<<<<<...<<...<
<<<.<.....>.>>>>...>>>>>>>>..........<<<<<<..<<<<<
<<<<<...>>>>>>>>>>...<<..))>>.....>>>>>>.>>>.<<<..
....<<<<....>>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<
<<<<..<<<<<<<<<<......<<........>>..........<<<<<<
<......<<<<<<<<<....>>>>>>>>>.<<<.<<<..<<<<<<.....
..<<<<<<<<<....>>>..<<<<......>>>>..>>>>>>.....<<<
<.<<<<<<<...<...<<<.....>>>>.....>>>>>>>.....<<<<<
....>>>>>........>>>>.........>>>...>>>>>>>>>...>>
>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<..<<..<<<<.
...>>>>..>>....>>>.....>>>>>>>......<....<<<<<<<..
......>>>>>>>....>.....>>>>>>....<<<<<<<..........
>>>>>>>......>>...>>>>>>>>>>.>>....<..<<.<.<<<<.<<
<..<<<<<<<<<<<<<...<.<<<<....<<<....>>>.>>>>.>..>>
>>>>>>>>>>>..>>>.>>>>..>.>>...>.....<<<<<<<<<....>
>>>>>>>>...

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4v95 Structural basis for the rescue of stalled ribosomes: structure of YaeJ bound to the ribosome.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): F10 H14 W34 R36 R37 Y52 N53 G54 K55 K70 G72 E73 T77 R78 T79 Y80 H83
• Binding residue (residue number reindexed from 1): F1 H5 W25 R27 R28 Y43 N44 G45 K46 K61 G63 E64 T68 R69 T70 Y71 H74

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4v95, PDBe:4v95, PDBj:4v95
• PDBsum: 4v95
• PubMed: 22422986
• UniProt: Q5SHP2|RS19_THET8 Small ribosomal subunit protein uS19 (Gene Name=rpsS)