Structure of PDB 8jiv Chain CR Binding Site BS01

Receptor Information

>8jiv Chain CR (length=148) Species: 4565 (Triticum aestivum)

VSLKLQKRLASSVLKCGKGKVWLDPNEVNEISMANSRQNIRKLVKDGFII
RKPQKIHSRSRARRAHEAKQKGRHSGYGKRRGTREARLPTKILWMRRMRV
LRRLLRKYREAKKIDKHMYHDMYLKVKGNMFKNKRVLMESIHKSKAEK

Ligand information

>8jiv Chain Aa (length=2863)

gcgaccccaggucaggcgggacuacccgcugaguuuaagcauauaaauaa
gcggaggagaagaaacuuacaaggauuccccuaguaacggcgagcgaacc
gggagcagcccagcuugagaaucgggcggcugugccguccgaauuguagu
cuggagaggcguccugcgacggaccgggcccaaguccccuggaaaggggc
gccugggagggugagagccccguccggcccggacccugucgcgaggcgcc
ucaacgagucggguuguuugggaaugcagcccaaaucgggcgguagacuc
cguccaaggcuaaauacaggcgagagaccgauagcgaacaaguaccgcga
gggaaagaugaaaaggacuuugaaaagagagucaaaagugcuugaaauug
ccgggagggaagcggaugggggccggcgaugcgccccggccguaugcgga
acggcugguccgccgcucggcucgggguguggacuguugucggccgcgcc
ggcggccaaagcccgggggcccuaggugcccccgguggccgucgucggca
cggccgcgcgccuaaaggcgugucccucggggcacugcgcugcaacggcc
ugcgggcuccccauccgacccgucuugaaacacggaccaaggagucugac
augcgugcgagucgacggguucugaaaccugggaugcgcaaggaagcuga
cgagcgggaggcccugggccgcaccgcuggccgacccugaucuucuguga
aggguucgaguuggagcacgccugucgggacccgaaagauggugaacuau
gccugagcggggcgaagccagaggcucugguggaggcucgaagcgauacu
gacgugcaaaucguucgucugacuuggguauaggggcgaaagacuaaucg
aaccaucuaguagcugguucccuccgaaguuucccucaggauagcuggag
cccaacgaguucuaucagguaaagccaaugauuagaggccuauucucaaa
cuuuaaauagguaggauggcgcggcugcuucggugagccgugccauggaa
ucgggcuccaagugggccauuuuugguaagcagaacuggcgaugcgggau
gaaccggaagccggguuacggugcccaacugcgcgcuaaccuagaaccca
caaaggguguuggucgaacuagccccgaaaauggauggcgcugaagcgcg
cgacccacacccggccaucugggcgagcgccaugccccgaugaguaggag
ggcgcggcggccgcugcaaaacccggggcgcgagcccgggcggagcggcc
gucggugcagaucuuggugguaguagcaaauauucaaaugagaacuuuga
aggccgaagaggagaaagguuccaugugaacggcacuugcacauggguaa
gccgauccuaagggacgggguaaccccggcagauagcgcacgcgccccga
aagggaaucggguuaagauuucccgagccgggauguggcgguugacggcg
acguuaggaaguccggagacgccggcgggggccucgggaagaguuaucuu
uucugcuuaacggccugccaacccuggaaacgguucagccggagguaggg
uccaguggccggaagagcaccggguguccggugcgcccccggcggcccau
gaaaauccggaggaccgccguucacgcccggucguacucauaaccgcauc
aggucuccaaggugaacagccucuggccaauggaacaauguaggcaaggg
aagucggcaaaacggauccguaacuucgggaaaaggauuggcucugagga
ccgacucagaacugguacggacaaggggaauccgacuguuuaauuaaaac
aaagcauugcgaugguccucgcggaugcugacgcaaugugauuucugccc
agugcucugaaugucaaagugaagaaauucaaccaagcgcggguaaacgg
caagguagccaaaugccucgucaucuaauuagugacgcgcaugaauggau
uaacgagauucccacugucccugucuacuauccagcgaaaccacagccaa
gggaacgggcuuggcggaaucagcggggaaagaagacccuguugagcuug
acucuaguccgacuuugugaauacuuauuccgugggucggaaguuuuggc
uccaaggccggccgauccgggcggaagacauugucagguggggaguuugg
cuggggcggcacaucuguuaaaagauaacgcagguguccuaagaugagcu
caacgagaacagaaaucucguguggaacaaaaggguaaaagcucguuuga
uucugauuuccaguacgaauacgaaccgugaaagcguggccuaucgaucc
uuuagcuucggaguuugaagcuagaggugucagaaaaguuaccacaggga
uaacuggcuuguggcagccaagcgauagcgacguugcuuuuugauccuuc
gaugucggcucuuccuaucauugugaagcagaauucaccaaguguuggau
uguucacccaccaauagggaacgugagcuggguuuagaccgucgugagac
agguuaguuuuacccuacugaugacagugucgcgauaguaauucaaccua
guacgagaggaaccguugauucacacaauuggucaucgcgcuugguugaa
aagccaguggcgcgaagcuaccgugugccggauuaugacugaacccucua
agucagaauccaagcuagcaugcgacgccucgacccacguuaggcccaag
ggcccgugggccgaagugcagcucccuucccgaauccuuugcagacgacu
uaagacggggcauuguaaguggcagagugacgauccacugagauccagcc
ccgucgcauucgu

........................<<<<<<.....<<....>>......>
>>>>.>.........<<....>>..<<<<<.......<.....>......
>>>>>..<<<....<......<<<<<<<<<...>>>>>>>>>......>.
.....<<<<<<<<.<<<<<<<<.<<<<<<<.....<<<<......>>>>.
((......<<<.....))>>>....>>>>>>>...>>>>>>>>.>>>>>>
>>........<<<<<<<........>>>>>>>.....<<<<<..((((((
>>>>>..>>>..................................<<....
>>...............<<<<....>>>>.....................
............<<<<<<<<<<<<......<.<<<<<<.<.......<<.
..<.>...>>........>..>.>>>>>.>.<.<<<<<<......<<...
.<..<......<..<<..<.......>.>>...>.....>..>....>>.
.......<<.<.<....>.>....<......>......>>..>>>>>>.>
....>>>.>>>>>>>>>..<<<<........>>>><<<<<<....<<<<<
<.<<<<<<..<<...<<<<<<....>>>>>>....>>...<<....>>..
..<<<<<..<<<<<.>>>>>...>>>>><<<<<<<<.....<....>...
..>>.>>>.>>>...>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<
<<<<<<<..<<<....<<<<<<<.>>>>>>>.....>>>...<<<<.<<.
<<........>>>>>>>>....>>>>>>>>>...<<.......>>.....
.>>>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<
.<<..<<<.<<<<<<<.....<<<....<<<..<.<<..>>.>..>>>..
>>.>.....>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>
>>>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<...
...>>>...<<<<<<<...<<<(((.....<<<<<<.......<......
....<<<.<<<<<<...>>>>>>>>>..........))).>....>>>>>
>.>>>...>>>>>>><<<<.<<<<.........>>>>.>>>>....>>>>
>><.<<<<<<<<<<.......<<<.<<<<....>>>>>>>....>>>>>>
>>>>..>.....>>>>>><<.....<<.....<<<<<<.......>>>>>
><<<<<....<<<<<.<<...<<<<<....<<.....>>..>>>>>....
((<<<<<<...<<<...<<<....>>>........<<<..>>>..>>>..
..>>...<<<<<.........>>>>>))<<<<.<<<<<<<<...<<<...
.>>>...<...<<<<<<...<<<<.<<<<<<<.<<<<<.<<<<...[[..
>>>>.><...<<<<<.....<<<<<<....<<<<...>>>>....>>>>>
>.....>>>>>....><<<<<.>>>>>>>>>...>>>>>>>.>>>>....
.....>>>>>>...>..>>>>.>>>>>>>>>>>>..>>.....<<.....
.>>>>>>>.<<<<.....>>>>.>>>>>..<<<<.....<<<<<<<.<<.
........<.....<<<<<....<<..>>.....>>>>><<.<<<<<<..
....>>>>>>.>>............<<<<<<<<<..<<<.........>>
>..<<<<<<<....<<<<....>>>>......>>>>>>>...<<..<<<.
.<<<<<<....<<<.....<<<<....>>>>.....>>>.>>>>..>>>>
>......<...........<<<<<.......>>>>>.....>.....>>.
...>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<
<........>>>>>....<<<<<..<<<.<<......<<<<.<<<<<<..
<<<<<.<<<.<<<...<<<..>>>...<<<<<.....<<<<.........
.....<<<..>>>..>>>>..>>>>>......>>>.>>>..>>>>>...<
<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<<<<.
..<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...
<<<..<<<<.......>>>>..>>>.<<<....>>>.>>))).......<
<<<<<<<<<.))))))>>>>>>>>>>..........>>>>..>>>>>>..
.....<<.<.<.<<<<<<...<<....>>...>>>>>>...>>.>.>...
....<<<<<..<<<<<..<<<.<<<<<......>>>>>...>>>.<<<..
.......>>>.....>>>>>......>>>.>>....<<<<<<<....>>>
.>>>>....>>..>>>.>>>>>....<<<<<<<..<<<<...<<<<<...
..............>>>>>..<<<<<...<<<<....<<<<<<<<<.]].
..>>>>..>>>>>...>>>>..>>>>>..<<<...<<<<<..........
>>>>>...>>>..>>>>....>>>>>>>.....<<.......<..>....
>>.............................<<<<..<............
.>.<<<<<<<.<<<....<<<<.<..<<.>>..>>>>>...>>>....>>
>>>>>..>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8jiv Cryo-EM structure of wheat ribosome reveals unique features of the plant ribosomes.
• Resolution: 2.84 Å
• Binding residue (original residue number in PDB): S3 L4 K5 K8 R9 G18 K19 K21 W23 L24 S37 R38 R42 Q55 K56 I57 H58 S59 R60 S61 R62 R64 R65 H67 R74 H75 G77 Y78 G79 K80 R81 R82 G83 T84 R85 A87 R88 L89 P90 K92 I93 W95 M96 R100 V101 R103 R104 R110 K117 H118 Y120 H121 Y124 L125 K126 K128 G129 N130 N134 K135
• Binding residue (residue number reindexed from 1): S2 L3 K4 K7 R8 G17 K18 K20 W22 L23 S36 R37 R41 Q54 K55 I56 H57 S58 R59 S60 R61 R63 R64 H66 R73 H74 G76 Y77 G78 K79 R80 R81 G82 T83 R84 A86 R87 L88 P89 K91 I92 W94 M95 R99 V100 R102 R103 R109 K116 H117 Y119 H120 Y123 L124 K125 K127 G128 N129 N133 K134

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8jiv, PDBe:8jiv, PDBj:8jiv
• PDBsum: 8jiv
• PubMed: 38458197
• UniProt: A0A3B6C2W3