Structure of PDB 8jiv Chain CP Binding Site BS01

Receptor Information

>8jiv Chain CP (length=153) Species: 4565 (Triticum aestivum)

VKYSTDPANPTKSAKAMGRDLRVHFKNTRETAFALRKMSLNKAKRYLEDV
LAHKQAIPFRRYCRGVGRTAQVKNRQPNGQGRWPAKSAKFVLDLLKNAES
NAEVKGLDVDALYISHIQVNQAQKQRRRTYRAHGRINPYMSNPCHIELIL
SEK

Ligand information

>8jiv Chain Aa (length=2863)

gcgaccccaggucaggcgggacuacccgcugaguuuaagcauauaaauaa
gcggaggagaagaaacuuacaaggauuccccuaguaacggcgagcgaacc
gggagcagcccagcuugagaaucgggcggcugugccguccgaauuguagu
cuggagaggcguccugcgacggaccgggcccaaguccccuggaaaggggc
gccugggagggugagagccccguccggcccggacccugucgcgaggcgcc
ucaacgagucggguuguuugggaaugcagcccaaaucgggcgguagacuc
cguccaaggcuaaauacaggcgagagaccgauagcgaacaaguaccgcga
gggaaagaugaaaaggacuuugaaaagagagucaaaagugcuugaaauug
ccgggagggaagcggaugggggccggcgaugcgccccggccguaugcgga
acggcugguccgccgcucggcucgggguguggacuguugucggccgcgcc
ggcggccaaagcccgggggcccuaggugcccccgguggccgucgucggca
cggccgcgcgccuaaaggcgugucccucggggcacugcgcugcaacggcc
ugcgggcuccccauccgacccgucuugaaacacggaccaaggagucugac
augcgugcgagucgacggguucugaaaccugggaugcgcaaggaagcuga
cgagcgggaggcccugggccgcaccgcuggccgacccugaucuucuguga
aggguucgaguuggagcacgccugucgggacccgaaagauggugaacuau
gccugagcggggcgaagccagaggcucugguggaggcucgaagcgauacu
gacgugcaaaucguucgucugacuuggguauaggggcgaaagacuaaucg
aaccaucuaguagcugguucccuccgaaguuucccucaggauagcuggag
cccaacgaguucuaucagguaaagccaaugauuagaggccuauucucaaa
cuuuaaauagguaggauggcgcggcugcuucggugagccgugccauggaa
ucgggcuccaagugggccauuuuugguaagcagaacuggcgaugcgggau
gaaccggaagccggguuacggugcccaacugcgcgcuaaccuagaaccca
caaaggguguuggucgaacuagccccgaaaauggauggcgcugaagcgcg
cgacccacacccggccaucugggcgagcgccaugccccgaugaguaggag
ggcgcggcggccgcugcaaaacccggggcgcgagcccgggcggagcggcc
gucggugcagaucuuggugguaguagcaaauauucaaaugagaacuuuga
aggccgaagaggagaaagguuccaugugaacggcacuugcacauggguaa
gccgauccuaagggacgggguaaccccggcagauagcgcacgcgccccga
aagggaaucggguuaagauuucccgagccgggauguggcgguugacggcg
acguuaggaaguccggagacgccggcgggggccucgggaagaguuaucuu
uucugcuuaacggccugccaacccuggaaacgguucagccggagguaggg
uccaguggccggaagagcaccggguguccggugcgcccccggcggcccau
gaaaauccggaggaccgccguucacgcccggucguacucauaaccgcauc
aggucuccaaggugaacagccucuggccaauggaacaauguaggcaaggg
aagucggcaaaacggauccguaacuucgggaaaaggauuggcucugagga
ccgacucagaacugguacggacaaggggaauccgacuguuuaauuaaaac
aaagcauugcgaugguccucgcggaugcugacgcaaugugauuucugccc
agugcucugaaugucaaagugaagaaauucaaccaagcgcggguaaacgg
caagguagccaaaugccucgucaucuaauuagugacgcgcaugaauggau
uaacgagauucccacugucccugucuacuauccagcgaaaccacagccaa
gggaacgggcuuggcggaaucagcggggaaagaagacccuguugagcuug
acucuaguccgacuuugugaauacuuauuccgugggucggaaguuuuggc
uccaaggccggccgauccgggcggaagacauugucagguggggaguuugg
cuggggcggcacaucuguuaaaagauaacgcagguguccuaagaugagcu
caacgagaacagaaaucucguguggaacaaaaggguaaaagcucguuuga
uucugauuuccaguacgaauacgaaccgugaaagcguggccuaucgaucc
uuuagcuucggaguuugaagcuagaggugucagaaaaguuaccacaggga
uaacuggcuuguggcagccaagcgauagcgacguugcuuuuugauccuuc
gaugucggcucuuccuaucauugugaagcagaauucaccaaguguuggau
uguucacccaccaauagggaacgugagcuggguuuagaccgucgugagac
agguuaguuuuacccuacugaugacagugucgcgauaguaauucaaccua
guacgagaggaaccguugauucacacaauuggucaucgcgcuugguugaa
aagccaguggcgcgaagcuaccgugugccggauuaugacugaacccucua
agucagaauccaagcuagcaugcgacgccucgacccacguuaggcccaag
ggcccgugggccgaagugcagcucccuucccgaauccuuugcagacgacu
uaagacggggcauuguaaguggcagagugacgauccacugagauccagcc
ccgucgcauucgu

........................<<<<<<.....<<....>>......>
>>>>.>.........<<....>>..<<<<<.......<.....>......
>>>>>..<<<....<......<<<<<<<<<...>>>>>>>>>......>.
.....<<<<<<<<.<<<<<<<<.<<<<<<<.....<<<<......>>>>.
((......<<<.....))>>>....>>>>>>>...>>>>>>>>.>>>>>>
>>........<<<<<<<........>>>>>>>.....<<<<<..((((((
>>>>>..>>>..................................<<....
>>...............<<<<....>>>>.....................
............<<<<<<<<<<<<......<.<<<<<<.<.......<<.
..<.>...>>........>..>.>>>>>.>.<.<<<<<<......<<...
.<..<......<..<<..<.......>.>>...>.....>..>....>>.
.......<<.<.<....>.>....<......>......>>..>>>>>>.>
....>>>.>>>>>>>>>..<<<<........>>>><<<<<<....<<<<<
<.<<<<<<..<<...<<<<<<....>>>>>>....>>...<<....>>..
..<<<<<..<<<<<.>>>>>...>>>>><<<<<<<<.....<....>...
..>>.>>>.>>>...>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<
<<<<<<<..<<<....<<<<<<<.>>>>>>>.....>>>...<<<<.<<.
<<........>>>>>>>>....>>>>>>>>>...<<.......>>.....
.>>>>>>...>..>.>>..<<<<<<....<.<<<....>>>.><<<<<<<
.<<..<<<.<<<<<<<.....<<<....<<<..<.<<..>>.>..>>>..
>>.>.....>>>>>...<<<<<<<<<.<......>>>>>>>>>>....>>
>>>>>>>>>>.>>..<<<<.<<<<<.....>>>>>.>>>>....<<<...
...>>>...<<<<<<<...<<<(((.....<<<<<<.......<......
....<<<.<<<<<<...>>>>>>>>>..........))).>....>>>>>
>.>>>...>>>>>>><<<<.<<<<.........>>>>.>>>>....>>>>
>><.<<<<<<<<<<.......<<<.<<<<....>>>>>>>....>>>>>>
>>>>..>.....>>>>>><<.....<<.....<<<<<<.......>>>>>
><<<<<....<<<<<.<<...<<<<<....<<.....>>..>>>>>....
((<<<<<<...<<<...<<<....>>>........<<<..>>>..>>>..
..>>...<<<<<.........>>>>>))<<<<.<<<<<<<<...<<<...
.>>>...<...<<<<<<...<<<<.<<<<<<<.<<<<<.<<<<...[[..
>>>>.><...<<<<<.....<<<<<<....<<<<...>>>>....>>>>>
>.....>>>>>....><<<<<.>>>>>>>>>...>>>>>>>.>>>>....
.....>>>>>>...>..>>>>.>>>>>>>>>>>>..>>.....<<.....
.>>>>>>>.<<<<.....>>>>.>>>>>..<<<<.....<<<<<<<.<<.
........<.....<<<<<....<<..>>.....>>>>><<.<<<<<<..
....>>>>>>.>>............<<<<<<<<<..<<<.........>>
>..<<<<<<<....<<<<....>>>>......>>>>>>>...<<..<<<.
.<<<<<<....<<<.....<<<<....>>>>.....>>>.>>>>..>>>>
>......<...........<<<<<.......>>>>>.....>.....>>.
...>>.>>>>>>>..>..>>.>>>>>>>..>>>>>>....>>....<<<<
<........>>>>>....<<<<<..<<<.<<......<<<<.<<<<<<..
<<<<<.<<<.<<<...<<<..>>>...<<<<<.....<<<<.........
.....<<<..>>>..>>>>..>>>>>......>>>.>>>..>>>>>...<
<<.....>>>..<<<<<<...........>>>>>>..<<...<<<<<<<.
..<<<<<<.......>>>>>>.....<<...(((>>....>>>>>>>...
<<<..<<<<.......>>>>..>>>.<<<....>>>.>>))).......<
<<<<<<<<<.))))))>>>>>>>>>>..........>>>>..>>>>>>..
.....<<.<.<.<<<<<<...<<....>>...>>>>>>...>>.>.>...
....<<<<<..<<<<<..<<<.<<<<<......>>>>>...>>>.<<<..
.......>>>.....>>>>>......>>>.>>....<<<<<<<....>>>
.>>>>....>>..>>>.>>>>>....<<<<<<<..<<<<...<<<<<...
..............>>>>>..<<<<<...<<<<....<<<<<<<<<.]].
..>>>>..>>>>>...>>>>..>>>>>..<<<...<<<<<..........
>>>>>...>>>..>>>>....>>>>>>>.....<<.......<..>....
>>.............................<<<<..<............
.>.<<<<<<<.<<<....<<<<.<..<<.>>..>>>>>...>>>....>>
>>>>>..>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8jiv Cryo-EM structure of wheat ribosome reveals unique features of the plant ribosomes.
• Resolution: 2.84 Å
• Binding residue (original residue number in PDB): Y4 K16 M18 R23 H25 F26 K27 R30 K43 R46 R62 Y63 R65 G66 G68 R69 A71 K74 N75 P78 Q81 R83 W84 P85 A86 K87 N98 S101 N102 Q119 V120 K125 R128 R129 T130 Y131 R132 A133 H134 G135 I137 N138 P139 Y140 M141 S142
• Binding residue (residue number reindexed from 1): Y3 K15 M17 R22 H24 F25 K26 R29 K42 R45 R61 Y62 R64 G65 G67 R68 A70 K73 N74 P77 Q80 R82 W83 P84 A85 K86 N97 S100 N101 Q118 V119 K124 R127 R128 T129 Y130 R131 A132 H133 G134 I136 N137 P138 Y139 M140 S141

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8jiv, PDBe:8jiv, PDBj:8jiv
• PDBsum: 8jiv
• PubMed: 38458197
• UniProt: W5FSM8